Continuous cultures

Biomining is a biotechnological process carried out in many parts of the world that exploits acid loving microorganisms to extract metals from sulphide minerals. One industrial biomining method is called ‘heap bioleaching’ where typically copper containing minerals are piled into very large heaps, acid and microorganisms are added to the top and the soluble metal is collected at the heap base.

The role of the different types of microbes in the process is to speed up metal solubilisation by oxidising
...

The raw data generated in the scope of the SysMetEx project for RNAseq, proteomics, and imaging analysis.
The data was generated on single and mixed species cultures of A. Caldus, L.ferriphilum, and/or S.thermosulfidooxidans.
Raw RNA data is combined in an ENA umbrella study summarising all short read data generated in the project.
Raw proteomics data is provided for distinct conditions at the pride repository.
Imaging data is provided for distinct conditions at a zenodo repository.

Proteomics data for samples derived from continuous cultures

RNAseq raw data derived from continuous culture samples

Umbrella study for all RNAseq rawdata
Separate projects can be accessed under "component projects"

Pride project for raw and search data for AXX-Cn samples:
LNU-AXX-Si00-CnB-P-B6ST-Pr_180min
LNU-AXX-Si00-CnB-P-B7ST-Pr_180min
LNU-AXX-Si00-CnB-P-B8ST-Pr_180min

Aceton precipitation and protein digestion for proteomic workflow

steps conducted to pellet cells for RNA and protein extraction

This file describes the naming system used to uniquely identify samples

Protocol for the extraction of biomolecules (DNA,RNA,protein,metabolties) from the same sample. Originally intended for lipid accumulating organisms (LAO) from wastewater treatment plant samples.
So far succesfully used for pelleted cells from planktonic cultures.