SysMetEx - Dataset collection

The main objective of the ERANET proposal Systems Biology Applications - ERASysAPP (app = application = translational systems biology) is to promote multidimensional and complementary European systems biology projects, programmes and research initiatives on a number of selected research topics. Inter alia, ERASysAPP will initiate, execute and monitor a number of joint transnational calls on systems biology research projects with a particular focus on applications - or in other words so called ...

Biomining is a biotechnological process carried out in many parts of the world that exploits acid loving microorganisms to extract metals from sulphide minerals. One industrial biomining method is called ‘heap bioleaching’ where typically copper containing minerals are piled into very large heaps, acid and microorganisms are added to the top and the soluble metal is collected at the heap base.

The role of the different types of microbes in the process is to speed up metal solubilisation by oxidising ...

Supplemental files for the publication of the dataset collection.

Rawdata for RNAseq derived from biofilms on chalcopyrite grains

Raw proteomics data derived from planktonic cells in chalcopyrite leaching experiments

Proteomics data for samples derived from continuous cultures

Link to RNAseq raw data

RNAseq raw data derived from continuous culture samples

Supplemental files for the publication

Links to additional gitlab repositories

Umbrella study for all RNAseq rawdata Separate projects can be accessed under "component projects"

Overview of the quality control for the RNAseq short read data after quality filtering of the reads

Overview for quality control of mapping statistics, after mapping processed reads to reference genomes

Repository for code used in data analysis (mainly for RNAseq) and for generating summary tables and overviews.

Files for the reference genomes utilized in the analysis are summarised in this repository.

Overivew of RNAseq and proteomics samples with respective accessions to access the raw data on ENA or PRIDE respectively.

Pride project for raw and search data for AXX-Cn samples: LNU-AXX-Si00-CnB-P-B6ST-Pr_180min LNU-AXX-Si00-CnB-P-B7ST-Pr_180min LNU-AXX-Si00-CnB-P-B8ST-Pr_180min

Raw proteomics data for L. ferriphilum planktonic samples

Raw proteomics data for S. thermosulfidooxidans samples

Raw data for tertiary mixture, planktonic cells, proteomics

Raw data for , Ac + St or Lf + St planktonic cells from leaching experiments

Raw proteomics data for St + Ac planktonic cells

Raw data for Ac + St leaching experiments proteomics data, with Ac 10x inoculum sizing

Raw data for Ac+St planktonic cells, leaching experiments with St 10x incoulum sizing

Parameters used in maxQuant analysis

Overview of the quality control for the RNAseq short read data before quality filtering of the reads

Raw data for mixed culture biofilm proteomics

Samples: UDE-SAL8-7O-M-B-Pr-2 UDE-SAL8-7O-M-B-Pr UDE-SAL7-Pc20-7M-P-B-Pr_2uL UDE-SAL7-A7-M-B-Pr UDE-SAL7-7C-M-B-Pr UDE-SAL7-7A-M-B-Pr

Microscopy images of A.Caldus (A), L.ferriphilum (L), S.thermosulfidooxidans (S) biofilms on chalcopyrite particles. (individual,e.g. SXX or mixed, e.g. SAL, cultures)

Timepoints: Day1, Day3, Day7, Day14, Day21

Raw data for mixed cultures of S. thermosulfidooxidans and L.ferriphilum

Raw data for L.ferriphilum biofilm proteomics data

Aceton precipitation and protein digestion for proteomic workflow

SOP for bioleaching experiments carried out for microscopy analysis at UDE

steps conducted to pellet cells for RNA and protein extraction

This file describes the naming system used to uniquely identify samples

SOP for extracting DNA, RNA and Proteins from the mineral pellet of a bioleaching culture using hot acidic phenol. Current draft.

Protocol for the extraction of biomolecules (DNA,RNA,protein,metabolties) from the same sample. Originally intended for lipid accumulating organisms (LAO) from wastewater treatment plant samples. So far succesfully used for pelleted cells from planktonic cultures.

sampling procedure for mineral containing leaching experiments