steps conducted to pellet cells for RNA and protein extraction
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Created: 18th Mar 2015 at 14:51
Last updated: 12th Feb 2020 at 08:31
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Version 3 (latest) Created 29th May 2015 at 08:27 by Stephan Christel
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Expertise: Microbiology, Bioleaching
Tools: Microbiology, Microbial Ecology
The main objective of the ERANET proposal Systems Biology Applications - ERASysAPP (app = application = translational systems biology) is to promote multidimensional and complementary European systems biology projects, programmes and research initiatives on a number of selected research topics. Inter alia, ERASysAPP will initiate, execute and monitor a number of joint transnational calls on systems biology research projects with a particular focus on applications - or in other words so called ...
Projects: SysVirDrug, SysMilk, SysMetEx, MetApp, IMOMESIC, WineSys, CropClock, SYSTERACT, XyloCut, RootBook, ROBUSTYEAST, LEANPROT, ErasysApp Funders
Web page: https://www.cobiotech.eu/about-cobiotech/erasysapp
Biomining is a biotechnological process carried out in many parts of the world that exploits acid loving microorganisms to extract metals from sulphide minerals. One industrial biomining method is called ‘heap bioleaching’ where typically copper containing minerals are piled into very large heaps, acid and microorganisms are added to the top and the soluble metal is collected at the heap base.
The role of the different types of microbes in the process is to speed up metal solubilisation by oxidising ...
Programme: ERASysAPP
Public web page: http://sysmetex.eu/index.html
The raw data generated in the scope of the SysMetEx project for RNAseq, proteomics, and imaging analysis. The data was generated on single and mixed species cultures of A. Caldus, L.ferriphilum, and/or S.thermosulfidooxidans. Raw RNA data is combined in an ENA umbrella study summarising all short read data generated in the project. Raw proteomics data is provided for distinct conditions at the pride repository. Imaging data is provided for distinct conditions at a zenodo repository.
Submitter: Malte Herold
Studies: Biofilms on chalcopyrite grains, Continuous cultures, Planktonic cells, Supplemental Files
Assays: Links to code repositories, Microscopy imaging, Proteomics rawdata, Proteomics rawdata, Proteomics rawdata, RNAseq rawdata, RNAseq rawdata, RNAseq rawdata, Supplemental Files
Snapshots: Snapshot 1, Snapshot 2
Experimental data and all related material for the publication "Multi -omics reveal lifestyle of acidophile, mineral-oxidizing model species Leptospirillum ferriphilumT".
Submitter: Malte Herold
Studies: Omics_data_analysis
Assays: Experimental methods, Genomics, Proteomics, RNAseq
Snapshots: Snapshot 1
Submitter: Malte Herold
Investigation: SysMetEx - Dataset collection
Assays: Proteomics rawdata, RNAseq rawdata
Snapshots: No snapshots
This stores: Processed data files Links to raw data files Links to repositories containing applied workflows
Submitter: Malte Herold
Investigation: Multi -omics reveal lifestyle of acidophile, mi...
Assays: Experimental methods, Genomics, Proteomics, RNAseq
Snapshots: No snapshots
Collection for experimental SOPs
Submitter: Malte Herold
Assay type: Experimental Assay Type
Technology type: Technology Type
Investigation: Multi -omics reveal lifestyle of acidophile, mi...
Study: Omics_data_analysis
Organisms: No organisms
SOPs: 003 Biomolecular Extractions from LAO (Qiagen A..., SOP - Bioleaching in flasks (LNU), SOP - RNA/Protein sampling for continuous culture
Data files: No Data files
Snapshots: No snapshots
Proteomics data for samples derived from continuous cultures
Submitter: Malte Herold
Assay type: Proteomics
Technology type: Technology Type
Investigation: SysMetEx - Dataset collection
Study: Continuous cultures
Organisms: Acidithiobacillus caldus : Acidithiobacillus caldus ATCC 51756 (wild-type / wild-type)
SOPs: 003 Biomolecular Extractions from LAO (Qiagen A..., In solution digestion part, SOP - RNA/Protein sampling for continuous culture, Sample Identification Code
Data files: AXX_Cn Proteomics
Snapshots: No snapshots
RNAseq raw data derived from continuous culture samples
Submitter: Malte Herold
Assay type: Experimental Assay Type
Technology type: Technology Type
Investigation: SysMetEx - Dataset collection
Study: Continuous cultures
Organisms: Acidithiobacillus caldus : Acidithiobacillus caldus ATCC 51756 (wild-type / wild-type), Leptospirillum ferriphilum : Leptospirillum ferriphilum DSM 14647 (wild-type / wild-type), Sulfobacillus thermosulfidooxidans : Sulfobacillus thermosulfidooxidans DSM_9293 (wild-type / wild-type)
SOPs: 003 Biomolecular Extractions from LAO (Qiagen A..., SOP - RNA/Protein sampling for continuous culture, Sample Identification Code
Data files: Rawdata RNAseq
Snapshots: No snapshots