Multi -omics reveal lifestyle of acidophile, mineral-oxidizing model species Leptospirillum ferriphilumT

The main objective of the ERANET proposal Systems Biology Applications - ERASysAPP (app = application = translational systems biology) is to promote multidimensional and complementary European systems biology projects, programmes and research initiatives on a number of selected research topics. Inter alia, ERASysAPP will initiate, execute and monitor a number of joint transnational calls on systems biology research projects with a particular focus on applications - or in other words so called ...

Biomining is a biotechnological process carried out in many parts of the world that exploits acid loving microorganisms to extract metals from sulphide minerals. One industrial biomining method is called ‘heap bioleaching’ where typically copper containing minerals are piled into very large heaps, acid and microorganisms are added to the top and the soluble metal is collected at the heap base.

The role of the different types of microbes in the process is to speed up metal solubilisation by oxidising ...

This stores: Processed data files Links to raw data files Links to repositories containing applied workflows

RNAseq data for L.ferriphilum samples

Proteomics data for L.ferriphilum samples (continuous and with mineral)

Genomics data, for L.ferriphilum Sequencing of the genome and functional annotation

Collection for experimental SOPs

Filtering of data in Perseus and t-test between conditions Perseus save file

Maxquant output.

Proteomics data comparing LXX cultures cultivated on mineral and in continuous culture: mineral samples: LNU-LXX9-Si00-14B-P-B-Pr LNU-LXX9-Si00-14B-P-C-Pr LNU-LXX9-Si00-14B-P-D-Pr conitnuous culture samples: LNU-LXX9-Si00-CnA-P-B2-Pr LNU-LXX9-Si00-CnA-P-B3-Pr LNU-LXX9-Si00-CnA-P-B4-Pr LNU-LXX9-Si00-CnA-P-B5-Pr LNU-LXX9-Si00-CnA-P-B6-Pr LNU-LXX9-Si00-CnA-P-B7-Pr

LFQ intensities from maxquant from combined search (mineral and continuous samples), filtered (at least 3 values in each condition), log-transformed

LFQ intensities from maxquant from combined search (mineral and continuous samples), log-transformed

Raw data and assembly for pacbio sequencing of L.ferriphilum.

Link to the repository for functional annotation

Genbank file for the L.ferriphilum chromosome, annotated with prokka 1.12beta

Table combining data for L.ferriphilum. RNAseq data, Proteomics LFQs, Functional annotation

Comparison between axenic and planktonic readcounts with deseq2

Comparison for LFQs with t-test in Perseus

Raw read files for samples

Normalized counts (tpm) for LF samples with mineral

Normalized counts (tpm) for L.ferriphilum samples from continuous culture.

Repository for omics data analysis

steps conducted to pellet cells for RNA and protein extraction

Protocol for the extraction of biomolecules (DNA,RNA,protein,metabolties) from the same sample. Originally intended for lipid accumulating organisms (LAO) from wastewater treatment plant samples. So far succesfully used for pelleted cells from planktonic cultures.

Initial configuration and parameters of the Bioleaching