SEEK ID: https://fairdomhub.org/assays/1022
Experimental assay
Projects: SysMetEx
Investigation: SysMetEx - Dataset collection
Study: Biofilms on chalcopyrite grains
Assay position:
Assay type: Proteomics
Technology type: Technology Type
Organisms: Acidithiobacillus caldus : Acidithiobacillus caldus ATCC 51756 (wild-type / wild-type), Leptospirillum ferriphilum : Leptospirillum ferriphilum DSM 14647 (wild-type / wild-type), Sulfobacillus thermosulfidooxidans : Sulfobacillus thermosulfidooxidans DSM_9293 (wild-type / wild-type)
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Created: 6th May 2019 at 15:05
Last updated: 12th Feb 2020 at 12:47
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- Data files (3)
- SOPs (4)
Projects: SysMetEx
Institutions: University of Luxembourg
The main objective of the ERANET proposal Systems Biology Applications - ERASysAPP (app = application = translational systems biology) is to promote multidimensional and complementary European systems biology projects, programmes and research initiatives on a number of selected research topics. Inter alia, ERASysAPP will initiate, execute and monitor a number of joint transnational calls on systems biology research projects with a particular focus on applications - or in other words so called ...
Projects: SysVirDrug, SysMilk, SysMetEx, MetApp, IMOMESIC, WineSys, CropClock, SYSTERACT, XyloCut, RootBook, ROBUSTYEAST, LEANPROT, ErasysApp Funders
Web page: https://www.cobiotech.eu/about-cobiotech/erasysapp
Biomining is a biotechnological process carried out in many parts of the world that exploits acid loving microorganisms to extract metals from sulphide minerals. One industrial biomining method is called ‘heap bioleaching’ where typically copper containing minerals are piled into very large heaps, acid and microorganisms are added to the top and the soluble metal is collected at the heap base.
The role of the different types of microbes in the process is to speed up metal solubilisation by oxidising ...
Programme: ERASysAPP
Public web page: http://sysmetex.eu/index.html
The raw data generated in the scope of the SysMetEx project for RNAseq, proteomics, and imaging analysis. The data was generated on single and mixed species cultures of A. Caldus, L.ferriphilum, and/or S.thermosulfidooxidans. Raw RNA data is combined in an ENA umbrella study summarising all short read data generated in the project. Raw proteomics data is provided for distinct conditions at the pride repository. Imaging data is provided for distinct conditions at a zenodo repository.
Submitter: Malte Herold
Studies: Biofilms on chalcopyrite grains, Continuous cultures, Planktonic cells, Supplemental Files
Assays: Links to code repositories, Microscopy imaging, Proteomics rawdata, Proteomics rawdata, Proteomics rawdata, RNAseq rawdata, RNAseq rawdata, RNAseq rawdata, Supplemental Files
Snapshots: Snapshot 1, Snapshot 2
Submitter: Malte Herold
Investigation: SysMetEx - Dataset collection
Assays: Microscopy imaging, Proteomics rawdata, RNAseq rawdata
Snapshots: No snapshots
Submitter: Malte Herold
Provider Name: Not specified
Provider's strain ID: Not specified
Organism: Sulfobacillus thermosulfidooxidans
Genotypes: wild-type
Phenotypes: wild-type
Comment: Created again as "Sulfobacillus thermosulfidooxidans DSM 9293" seemed to be broken
Raw data for mixed culture biofilm proteomics
Samples: UDE-SAL8-7O-M-B-Pr-2 UDE-SAL8-7O-M-B-Pr UDE-SAL7-Pc20-7M-P-B-Pr_2uL UDE-SAL7-A7-M-B-Pr UDE-SAL7-7C-M-B-Pr UDE-SAL7-7A-M-B-Pr
Investigations: SysMetEx - Dataset collection
Studies: Biofilms on chalcopyrite grains
Assays: Proteomics rawdata
Raw data for mixed cultures of S. thermosulfidooxidans and L.ferriphilum
Investigations: SysMetEx - Dataset collection
Studies: Biofilms on chalcopyrite grains
Assays: Proteomics rawdata
Raw data for L.ferriphilum biofilm proteomics data
Investigations: SysMetEx - Dataset collection
Studies: Biofilms on chalcopyrite grains
Assays: Proteomics rawdata
Aceton precipitation and protein digestion for proteomic workflow
Creators: Malte Herold, Mohamed El Hajjami
Submitter: Malte Herold
Investigations: SysMetEx - Dataset collection
Studies: Biofilms on chalcopyrite grains, Continuous cultures, Planktonic cells
Assays: Proteomics rawdata, Proteomics rawdata, Proteomics rawdata
This file describes the naming system used to uniquely identify samples
Creator: Stephan Christel
Submitter: Stephan Christel
Investigations: SysMetEx - Dataset collection
Studies: Biofilms on chalcopyrite grains, Continuous cultures, Planktonic cells
Assays: Proteomics rawdata, Proteomics rawdata, Proteomics rawdata, RNAseq rawdata, RNAseq rawdata, RNAseq rawdata
SOP for extracting DNA, RNA and Proteins from the mineral pellet of a bioleaching culture using hot acidic phenol. Current draft.
Creators: Malte Herold, Mario Vera, Soeren Bellenberg
Submitter: Malte Herold
Investigations: SysMetEx - Dataset collection
Studies: Biofilms on chalcopyrite grains
Assays: Proteomics rawdata, RNAseq rawdata
sampling procedure for mineral containing leaching experiments
Creator: Stephan Christel
Submitter: Stephan Christel
Investigations: SysMetEx - Dataset collection
Studies: Biofilms on chalcopyrite grains, Planktonic cells
Assays: Proteomics rawdata, Proteomics rawdata, RNAseq rawdata, RNAseq rawdata