DNA, RNA, Protein extraction from mineral samples Version 2
SOP for extracting DNA, RNA and Proteins from the mineral pellet of a bioleaching culture using hot acidic phenol.
Current draft.
SEEK ID: https://fairdomhub.org/sops/249?version=2
Filename:
Hot_Phenol_Protocol.docx
Format: Word document
Size: 102 KB
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Creators and Submitter
Views: 1617 Downloads: 118
Created: 1st Apr 2016 at 12:01
Last updated: 6th Feb 2020 at 17:36
Last used: 5th Aug 2020 at 17:55
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Related items
Projects: SysMetEx
Institutions: Ruhr University Bochum
Projects: SysMetEx
Institutions: University of Luxembourg
Projects: SysMetEx
Institutions: Universitity Duisburg-Essen
My research is intended to contribute to the elucidation of the physiological and molecular processes involved in the biofilm formation of acidophilic leaching bacteria with emphasis in their cell-cell communication mechanisms.
In SysMetEx, our role is to understand biofilm formation at a microscopical and OMICS levels, in order to optimize it.
Biomining is a biotechnological process carried out in many parts of the world that exploits acid loving microorganisms to extract metals from sulphide minerals. One industrial biomining method is called ‘heap bioleaching’ where typically copper containing minerals are piled into very large heaps, acid and microorganisms are added to the top and the soluble metal is collected at the heap base.
The role of the different types of microbes in the process is to speed up metal solubilisation by oxidising
...
Programme: ERASysAPP
Public web page: http://sysmetex.eu/index.html
The raw data generated in the scope of the SysMetEx project for RNAseq, proteomics, and imaging analysis.
The data was generated on single and mixed species cultures of A. Caldus, L.ferriphilum, and/or S.thermosulfidooxidans.
Raw RNA data is combined in an ENA umbrella study summarising all short read data generated in the project.
Raw proteomics data is provided for distinct conditions at the pride repository.
Imaging data is provided for distinct conditions at a zenodo repository.
Snapshots: Snapshot 1, Snapshot 2
Submitter: Malte Herold
Assay type: Proteomics
Technology type: Technology Type
Snapshots: No snapshots
Rawdata for RNAseq derived from biofilms on chalcopyrite grains
Submitter: Malte Herold
Assay type: Experimental Assay Type
Technology type: Technology Type
Snapshots: No snapshots
