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DNA, RNA, Protein extraction from mineral samples

DNA, RNA, Protein extraction from mineral samples
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SOP for extracting DNA, RNA and Proteins from the mineral pellet of a bioleaching culture using hot acidic phenol.
Current draft.

SEEK ID: https://fairdomhub.org/sops/249?version=2

Filename: Hot_Phenol_Protocol.docx

Format: Word document

Size: 102 KB

DOI: 10.15490/fairdomhub.1.sop.249.2

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Creators and Submitter

Creators

Malte Herold
Mario Vera
Soeren Bellenberg

Submitter

Malte Herold

Citation

Herold, M., Vera, M., & Bellenberg, S. (2018). DNA, RNA, Protein extraction from mineral samples. FAIRDOMHub. http://doi.org/10.15490/FAIRDOMHUB.1.SOP.249.2

License

Creative Commons Attribution 4.0

Activity

Views: 1920 Downloads: 137

Created: 1st Apr 2016 at 12:01

Last updated: 6th Feb 2020 at 17:36

Last used: 14th Apr 2021 at 08:00

Attributions

None

Related items

People (3)
Projects (1)
Investigations (1)
Studies (1)
Assays (2)

Soeren Bellenberg

Projects: SysMetEx

Institutions: Ruhr University Bochum

Expertise: Bioleaching, Molecular microbiology, Biochemistry

Tools: Fluorescence and confocal microscopy, cultivation, microbiology techniques

Malte Herold

Projects: SysMetEx

Institutions: University of Luxembourg

Mario Vera

Projects: SysMetEx

Institutions: Universitity Duisburg-Essen

Expertise: Microbiology, Molecular Biology, Molecular microbiology, Genomics, Proteomics, Systems Biology, Databases

Tools: Fluorescence and confocal microscopy, Molecular biology techniques (RNA/DNA/Protein), Microbiology, Molecular Biology, qRT-PCR, Proteomics

My research is intended to contribute to the elucidation of the physiological and molecular processes involved in the biofilm formation of acidophilic leaching bacteria with emphasis in their cell-cell communication mechanisms.
In SysMetEx, our role is to understand biofilm formation at a microscopical and OMICS levels, in order to optimize it.

SysMetEx

Biomining is a biotechnological process carried out in many parts of the world that exploits acid loving microorganisms to extract metals from sulphide minerals. One industrial biomining method is called ‘heap bioleaching’ where typically copper containing minerals are piled into very large heaps, acid and microorganisms are added to the top and the soluble metal is collected at the heap base.

The role of the different types of microbes in the process is to speed up metal solubilisation by oxidising
...

Programme: ERASysAPP

Public web page: http://sysmetex.eu/index.html

Organisms: Acidithiobacillus caldus, Sulfobacillus thermosulfidooxidans, Leptospirillum ferriphilum

SysMetEx - Dataset collection

SysMetEx

All contributors

Malte Herold

The raw data generated in the scope of the SysMetEx project for RNAseq, proteomics, and imaging analysis.
The data was generated on single and mixed species cultures of A. Caldus, L.ferriphilum, and/or S.thermosulfidooxidans.
Raw RNA data is combined in an ENA umbrella study summarising all short read data generated in the project.
Raw proteomics data is provided for distinct conditions at the pride repository.
Imaging data is provided for distinct conditions at a zenodo repository.

Snapshots: Snapshot 1, Snapshot 2

DOI: 10.15490/fairdomhub.1.investigation.292.2

Studies: Biofilms on chalcopyrite grains, Continuous cultures, Planktonic cells, Supplemental Files

Assays: Links to code repositories, Microscopy imaging, Proteomics rawdata, Proteomics rawdata, Proteomics rawdata, RNAseq rawdata, RNAseq rawdata, RNAseq rawdata, Supplemental Files