SOPs

401 SOPs visible to you, out of a total of 692

Determination of the kinetic parameters of the purified glycolytic isoenzymes of Saccharomyces cerevisiae

MOSES

General protocol for measuring the kinetic parameters of the purified glycolytic enzymes from Saccharomyces cerevisiae - SOP for measuring the kinetic parameters of the purified glycolytic isoenzymes

Creator: Hanan Messiha

Submitter: Maksim Zakhartsev

Investigations: No Investigations

Studies: No Studies

Assays: No Assays

Created: 2nd Mar 2010 at 22:29, Last updated: 1st Mar 2012 at 10:44

Determination of (cytosolic) enzyme activities

MOSES

Sample preparation - SOP for sampling, preparation of cell-free extracts, and determination of total extracted protein

Creator: Femke Mensonides

Submitter: Maksim Zakhartsev

Investigations: No Investigations

Studies: No Studies

Assays: No Assays

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Created: 2nd Mar 2010 at 22:22, Last updated: 1st Mar 2012 at 10:40

Determination of intracellular metabolites

MOSES

Sample preparation - SOP for sampling, preparation of cell extracts, and general assay set-up

Creator: Femke Mensonides

Submitter: Maksim Zakhartsev

Investigations: Kinetic analysis of metabolic system using tran..., Steady state metabolic fluxes and metabolite co...

Studies: Metabolic perturbation of the steady state cult..., Steady state concentrations of metabolites in y...

Assays: Cellular size and granularity during glucose pulse, Dynamics of intracellular metabolites during gl..., Dynamics of macromolecules during glucose pulse, Steady state concentrations of intracellular me...

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Created: 2nd Mar 2010 at 22:18, Last updated: 1st Mar 2012 at 10:35

Generation of RNAi cell lines in T.brucei brucei 2T1 cell line

SilicoTryp

We routinely select specific RNAi gene targets (400–600 bp) and primers using the RNAit software http://trypanofan.path.cam.ac.uk/software/RNAit.html. A single pair of PCR primers are designed that incorporate four selected restriction sites (not present in the RNAi target fragment) such that a single PCR product can be differentially digested and sequentially cloned. For example, using MCS1/2, the following primers could be used to clone antisense followed by sense fragments: Primer 1, XbaI–BamHI-5′ ...

Creator: Federico Rojas

Submitter: Federico Rojas

Investigations: Perturbing the biological system: developmental...

Studies: Targeted disruption and over expression of crit...

Assays: Generation of RNAi cell lines in T.brucei bruce...

Created: 25th Feb 2012 at 12:35, Last updated: 25th Feb 2012 at 12:35

NMR measurment

BaCell-SysMO

SOP for extracellular metabolite measurment

Creator: Hanna Meyer

Submitter: Hanna Meyer

Investigations: Multiomics study of Bacillus subtilis under osm...

Studies: B. subtilis_SysMo2_Chemostat_growthrate-salt

Assays: extracellular metabolites

Created: 17th May 2010 at 08:52, Last updated: 31st Jan 2012 at 10:40

Guidelines for Writing Standard Operating Procedures (SOP)

SysMO DB

Written Standard Operating Procedures provide workers with the operational information necessary to perform a job properly and ensure consistency in the operations. Standard Operating Procedures provide a historical record of steps in the how, why and when and serve as a training tool for teaching users.

Creator: Olga Krebs

Submitter: Olga Krebs

Investigations: No Investigations

Studies: No Studies

Assays: No Assays

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Created: 26th Oct 2009 at 14:48, Last updated: 22nd Nov 2011 at 13:55

ATP determination with ROCHE kit “ATP Bioluminescence Assay Kit CLS II”

MOSES

The kit is used for the quantitative detection of ATP in yeast cells by luciferase driven bioluminescence. ATP extraction is done by boiling cells in TE buffer.

Creators: Martin Valachovic, Cornelia Klein

Submitter: Walter Glaser

Investigations: Effect of Benzoic Acid on ATP Levels

Studies: ATP levels under 10 mM benzoic acid stress, ATP levels under 30 mM and 50 mM benzoic acid s..., Test different ATP extraction protocols and con...

Assays: ATP measurement under 10mM benzoic acid stress, ATP measurement under 30mM benzoic acid stress, ATP measurement under 50mM benzoic acid stress, Compare ATP extraction methods

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Created: 6th Jun 2010 at 15:40, Last updated: 25th Aug 2011 at 13:51

pH-BASED ESTIMATION OF YEAST ATPase ACTIVITY

TRANSLUCENT

No description specified

Creator: Lina Patricia Barreto Parra

Submitter: Lina Patricia Barreto Parra

Investigations: No Investigations

Studies: No Studies

Assays: No Assays

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Created: 6th Apr 2011 at 16:17

RNA isolation of Clostridium acetobutylicum

COSMIC

No description specified

Creator: Holger Janssen

Submitter: The JERM Harvester

Investigations: No Investigations

Studies: No Studies

Assays: No Assays

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Created: 12th Mar 2010 at 16:35, Last updated: 15th Nov 2010 at 17:13

Standard operating procedure to analyze the intracellular proteome of Clostridium acetobutylicum.

COSMIC

No description specified

Creators: Holger Janssen, Tomas Fiedler

Submitter: Holger Janssen

Investigations: The effect of pH upon the metabolic shift in Cl...

Studies: Effect of pH on the proteome

Assays: Comparison of the proteome between pH 5.7 (acid...

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Created: 15th Nov 2010 at 14:55

Spore batch generation

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No description specified

Creators: Trond Ellingsen, Øyvind Jakobsen, Per Bruheim, Håvard Sletta, Anders Øverby, Sven Even Borgos, Sunniva Hoel, Alexander Wentzel

Submitter: Jay Moore

Investigations: Metabolism of Streptomyces coelicolor (SysMO ST... and 1 hidden item

Studies: Timeseries 1 and 2 hidden items

Assays: metabolomics and 2 hidden items

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Created: 4th Nov 2010 at 14:10, Last updated: 4th Nov 2010 at 14:11

Metabolome extraction

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Creators: Trond Ellingsen, Øyvind Jakobsen, Per Bruheim, Håvard Sletta, Anders Øverby, Sven Even Borgos, Sunniva Hoel, Alexander Wentzel

Submitter: Jay Moore

Investigations: Metabolism of Streptomyces coelicolor (SysMO ST...

Studies: Timeseries 1

Assays: metabolomics

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Created: 4th Nov 2010 at 14:10

MCF derivatization

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Creators: Trond Ellingsen, Øyvind Jakobsen, Per Bruheim, Håvard Sletta, Anders Øverby, Sven Even Borgos, Sunniva Hoel, Alexander Wentzel

Submitter: Jay Moore

Investigations: Metabolism of Streptomyces coelicolor (SysMO ST...

Studies: Timeseries 1

Assays: metabolomics

Created: 4th Nov 2010 at 14:08

Proteomic Analysis of the Streptomyces coelicolor Proteome

STREAM

No description specified

Creators: Louise Thomas, Maggie Smith

Submitter: Jay Moore

Investigations: No Investigations

Studies: No Studies

Assays: No Assays

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Created: 26th May 2010 at 15:30

Total DNA Quantification

STREAM

No description specified

Creators: Louise Thomas, Maggie Smith

Submitter: Jay Moore

Investigations: No Investigations

Studies: No Studies

Assays: No Assays

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Created: 26th May 2010 at 15:29

Total RNA Quantification

STREAM

No description specified

Creators: Louise Thomas, Maggie Smith

Submitter: Jay Moore

Investigations: No Investigations

Studies: No Studies

Assays: No Assays

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Created: 26th May 2010 at 15:29

Total Protein Quantification

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No description specified

Creators: Louise Thomas, Maggie Smith

Submitter: Jay Moore

Investigations: No Investigations

Studies: No Studies

Assays: No Assays

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Created: 26th May 2010 at 15:28

Processing Streptomyces coelicolor cell pellets

STREAM

No description specified

Creators: Louise Thomas, Maggie Smith

Submitter: Jay Moore

Investigations: No Investigations

Studies: No Studies

Assays: No Assays

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Created: 26th May 2010 at 15:27

Contamination test of Streptomyces coelicolor culture

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No description specified

Creators: Per Bruheim, Trond Ellingsen, Sunniva Hoel, Øyvind Jakobsen, Håvard Sletta, Alexander Wentzel, Anders Øverby, Strøm, A

Submitter: Jay Moore

Investigations: Metabolism of Streptomyces coelicolor (SysMO ST... and 1 hidden item

Studies: Timeseries 1 and 8 hidden items

Assays: Online/offline measurements and 8 hidden items

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Created: 26th May 2010 at 15:24, Last updated: 26th May 2010 at 15:24

Generation of cell material from Streptomyces coelicolor culture for metabolome analysis

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Creators: Per Bruheim, Trond Ellingsen, Sunniva Hoel, Øyvind Jakobsen, Håvard Sletta, Alexander Wentzel, Anders Øverby, Strøm, A

Submitter: Jay Moore

Investigations: Metabolism of Streptomyces coelicolor (SysMO ST... and 1 hidden item

Studies: Timeseries 1 and 8 hidden items

Assays: Online/offline measurements and 8 hidden items

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Created: 26th May 2010 at 15:23

Generation of cell material from Streptomyces coelicolor culture for transcriptome analysis

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Creators: Per Bruheim, Trond Ellingsen, Sunniva Hoel, Øyvind Jakobsen, Håvard Sletta, Alexander Wentzel, Anders Øverby, Strøm, A

Submitter: Jay Moore

Investigations: Metabolism of Streptomyces coelicolor (SysMO ST... and 1 hidden item

Studies: Timeseries 1 and 8 hidden items

Assays: Online/offline measurements and 8 hidden items

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Created: 26th May 2010 at 15:22

Generation of cell material from Streptomyces coelicolor culture for proteome analysis

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Creators: Per Bruheim, Trond Ellingsen, Sunniva Hoel, Øyvind Jakobsen, Håvard Sletta, Alexander Wentzel, Anders Øverby, Strøm, A

Submitter: Jay Moore

Investigations: Metabolism of Streptomyces coelicolor (SysMO ST... and 1 hidden item

Studies: Timeseries 1 and 8 hidden items

Assays: Online/offline measurements and 8 hidden items

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Created: 26th May 2010 at 15:20

Cell dry weight (CDW) determination of Streptomyces coelicolor culture

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Creators: Per Bruheim, Trond Ellingsen, Sunniva Hoel, Øyvind Jakobsen, Håvard Sletta, Alexander Wentzel, Anders Øverby, Strøm, A

Submitter: Jay Moore

Investigations: Metabolism of Streptomyces coelicolor (SysMO ST... and 1 hidden item

Studies: Timeseries 1 and 8 hidden items

Assays: Online/offline measurements and 8 hidden items

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Created: 26th May 2010 at 15:19, Last updated: 26th May 2010 at 15:19

Sampling from Streptomyces coelicolor culture for subsequent offline analyses

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Creators: Per Bruheim, Trond Ellingsen, Sunniva Hoel, Øyvind Jakobsen, Håvard Sletta, Alexander Wentzel, Anders Øverby, Strøm, A

Submitter: Jay Moore

Investigations: Metabolism of Streptomyces coelicolor (SysMO ST... and 1 hidden item

Studies: Timeseries 1 and 8 hidden items

Assays: Online/offline measurements and 8 hidden items

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Created: 26th May 2010 at 15:17

Preparation of inoculum for Streptomyces coelicolor cultivation

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Creators: Per Bruheim, Trond Ellingsen, Sunniva Hoel, Øyvind Jakobsen, Håvard Sletta, Alexander Wentzel, Anders Øverby, Strøm, A

Submitter: Jay Moore

Investigations: Metabolism of Streptomyces coelicolor (SysMO ST... and 1 hidden item

Studies: Timeseries 1 and 8 hidden items

Assays: Online/offline measurements and 8 hidden items

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Created: 26th May 2010 at 15:15

Isolation of total RNA from yeast Saccharomyces cerevisiae for microarrays

MOSES

This protocol is designed to prepare sufficient amounts of high-quality total RNA from the yeast saccharomyces cerevisiae grown in liquid culture for analysis on microarrays.

Creators: Walter Glaser, Christa Gregori

Submitter: Walter Glaser

Investigations: No Investigations

Studies: No Studies

Assays: No Assays

Created: 26th Oct 2009 at 14:48, Last updated: 20th May 2010 at 08:45