LC-MS based absolute quantification of intracellular metabolites
Intracellular metabolites in T. brucei at different stage of cell growth have been quantified absolutely by isotope ratio based MS technique using uniformly 13C-labelled E. coli extract. This is the case study for method development of absolute quantification for metabolic flux analysis.
SEEK ID: https://fairdomhub.org/assays/204
Experimental assay
Projects: SilicoTryp
Investigation: Metabolite profiling, quantification and flux quantification
Study: Metabolic flux measurement
Assay position:
Assay type: Metabolomics
Technology type: Mass Spectrometry
Organisms: Trypanosoma brucei : Tbrucei427 (wild-type / wild-type)
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Created: 11th Dec 2012 at 15:23
Last updated: 8th Nov 2017 at 14:21
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Projects: SilicoTryp
Institutions: University of Glasgow
SysMO is a European transnational funding and research initiative on "Systems Biology of Microorganisms".
The goal pursued by SysMO was to record and describe the dynamic molecular processes going on in unicellular microorganisms in a comprehensive way and to present these processes in the form of computerized mathematical models.
Systems biology will raise biomedical and biotechnological research to a new quality level and contribute markedly to progress in understanding. Pooling European research ...
Projects: BaCell-SysMO, COSMIC, SUMO, KOSMOBAC, SysMO-LAB, PSYSMO, SCaRAB, MOSES, TRANSLUCENT, STREAM, SulfoSys, SysMO DB, SysMO Funders, SilicoTryp, Noisy-Strep
Web page: http://sysmo.net/
The SilicoTryp project aims at the creation of a “Silicon Trypanosome”, a comprehensive, experiment-based, multi-scale mathematical model of trypanosome physiology. Trypanosomes are blood-stream parasites transmitted by tsetse flies; they cause African sleeping sickness in humans and livestock. Currently available drugs have severe side effects, and the parasites are rapidly developing resistance. In this project, we collect a wide range of new experimental data on the parasite in its various ...
Programme: SysMO
Public web page: http://silicotryp.ibls.gla.ac.uk/wiki/Main_Page
The aims of this investigation is to quantify metabolites associated with pathways involved in stress responses for parameterising models of oxidative stress metabolism; the measurement of metabolic fluxes of metabolites of interest with intracellular concentrations
Submitter: Dong-Hyun Kim
Studies: Metabolic flux measurement, Targeted metabolite analysis, Untargeted metabolite analysis
Assays: Generation of uniformly 13C-labelled E. coli extract, Intracellular metabolite concentrations in T. brucei exposed to oxidativ..., Intracellular metabolite concentrations in T. brucei under pH stress, LC-MS based absolute quantification of extracellular metabolites, LC-MS based absolute quantification of intracellular metabolites, Metabolite profiling on T. brucei exposed to oxidative stress
Flux will be measured using the metabolomics platforms based on absolute quantification method (isotope ratio based MS technique) by LC-MS, using heavy-isotope labelled precursors of the metabolites of interest. For example, 15N labelled cysteine, glycine and glutamate will be used to determine rates of synthesis of glutathione. 15N-labelled methionine to measure S-adenosyl methionine (and its decarboxylated form, as well as methionine cycle intermediates). 15N labelled arginine is used as precursor ...
Submitter: Not available
Provider Name: Not specified
Provider's strain ID: Not specified
Organism: Trypanosoma brucei
Genotypes: wild-type
Phenotypes: wild-type
Comment: Not specified
Intracellular concentrations of 50 metabolites measured by LC-MS using isotope ratio based mass spectrometry technique
Sample preparation procedure for metabolic analysis on T. b. brucei 427 using LC/MS
Creator: Dong-Hyun Kim
Submitter: Dong-Hyun Kim
