Tbrucei427
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wild-type
wild-type
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Created: 22nd Feb 2012 at 18:10
Last updated: 18th May 2012 at 15:55

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This assay is for method development to quantify intra- and extra-cellular metabolites on T. brucei 427 bloodstream form using isotope ratio based MS technique with 13C-labelled E. coli extract
Submitter: Dong-Hyun Kim
Assay type: Metabolite Concentration
Technology type: Mass Spectrometry
Investigation: Metabolite profiling, quantification and flux q...
Study: Metabolic flux measurement
Organisms: Trypanosoma brucei : Tbrucei427 (wild-type / wild-type)
SOPs: Generation of uniformly 13C-labelled E. coli ex...
Data files: No Data files
Snapshots: No snapshots
Intracellular metabolites in T. brucei at different stage of cell growth have been quantified absolutely by isotope ratio based MS technique using uniformly 13C-labelled E. coli extract. This is the case study for method development of absolute quantification for metabolic flux analysis.
Submitter: Dong-Hyun Kim
Assay type: Metabolomics
Technology type: Mass Spectrometry
Investigation: Metabolite profiling, quantification and flux q...
Study: Metabolic flux measurement
Organisms: Trypanosoma brucei : Tbrucei427 (wild-type / wild-type)
SOPs: Sample preparation for intracellular metabolite...
Data files: Absolute intracellular concentrations of metabo...
Snapshots: No snapshots
Metabolite profiling on T. brucei exposed to methylene blue has been carried out using LC-MS to investigate metabolic changes caused by oxidative stress
Submitter: Dong-Hyun Kim
Assay type: Metabolite Profiling
Technology type: Liquid Chromatography Mass Spectrometry
Investigation: Metabolite profiling, quantification and flux q...
Organisms: Trypanosoma brucei : Tbrucei427 (wild-type / wild-type)
SOPs: Sample preparation for intracellular metabolite...
Data files: Metabolic changes by oxidative stress in T. b. ...
Snapshots: No snapshots
26 intracellular metabolites (amino acids, polyamines, TCA intermediates) in T. brucei exposed to methylene blue have been absolutely quantified using isotope ratio based MS technique.
Submitter: Dong-Hyun Kim
Assay type: Intracellular Metabolite Concentration
Technology type: Isotope Ratio Mass Spectrometry
Investigation: Metabolite profiling, quantification and flux q...
Organisms: Trypanosoma brucei : Tbrucei427 (wild-type / wild-type)
SOPs: Sample preparation for intracellular metabolite...
Data files: Absolute intracellular concentrations of metabo...
Snapshots: No snapshots
26 intracellular metabolites (amino acids, polyamines, TCA intermediates) in T. brucei under pH stress (pH8.7) have been absolutely quantified using isotope ratio based MS technique.
Submitter: Dong-Hyun Kim
Assay type: Intracellular Metabolite Concentration
Technology type: Isotope Ratio Mass Spectrometry
Investigation: Metabolite profiling, quantification and flux q...
Study: Targeted metabolite analysis
Organisms: Trypanosoma brucei : Tbrucei427 (wild-type / wild-type)
SOPs: Sample preparation for intracellular metabolite...
Data files: Absolute intracellular concentrations of metabo...
Snapshots: No snapshots
Extracellular metabolites in T. brucei at different stage of cell growth have been quantified absolutely by isotope ratio based MS technique using uniformly 13C-labelled E. coli extract. This is the case study for method development of absolute quantification for metabolic flux analysis.
Submitter: Dong-Hyun Kim
Assay type: Extracellular Metabolite Concentration
Technology type: Isotope Ratio Mass Spectrometry
Investigation: Metabolite profiling, quantification and flux q...
Study: Metabolic flux measurement
Organisms: Trypanosoma brucei : Tbrucei427 (wild-type / wild-type)
SOPs: Sample preparation procedure for footprint anal...
Data files: Concentrations of extracellular metabolites fro...
Snapshots: No snapshots