LC-MS based absolute quantification of extracellular metabolites

Extracellular metabolites in T. brucei at different stage of cell growth have been quantified absolutely by isotope ratio based MS technique using uniformly 13C-labelled E. coli extract. This is the case study for method development of absolute quantification for metabolic flux analysis.

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Created: 11th Dec 2012 at 16:21

Last updated: 8th Nov 2017 at 14:21

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