experimentally measured extracellular fluxes in yeast Saccharomyces cerevisiae in anaerobic glucose limited chemostat (D=0.1 h-1) on minimal medium
SEEK ID: https://fairdomhub.org/assays/37
Experimental assay
Projects: MOSES
Investigation: Steady state metabolic fluxes and metabolite concentrations in anaerobic chemostat of yeast Saccharomyces cerevisiae
Study: Steady state fluxes in yeast Saccharomyces cerevisiae in anaerobic chemostat
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Assay type: Metabolite Profiling
Technology type: HPLC
Organisms: Saccharomyces cerevisiae
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Created: 26th Apr 2010 at 14:14
Last updated: 8th Nov 2017 at 14:21
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Projects: MOSES, ExtremoPharm, ZucAt, GenoSysFat, DigiSal, EraCoBiotech 2 nd call proposal preparation, FAIRDOM & LiSyM & de.NBI Data Structuring Training
Institutions: University of Stuttgart, University of Hohenheim, Norwegian University of Life Sciences, Norwegian University of Science and Technology
https://orcid.org/0000-0002-7973-9902Expertise: Biochemistry, coupling metabolome and environome, rapid sampling experiments, Systems Biology, carbon metabolism, Stoichiometric modelling, Proteomics, Metabolomics, yeast, fungi, Dynamics and Control of Biological Networks
Tools: Biochemistry and protein analysis, Metabolomics, Matlab, Fermentation, Chromatography, Material balance based modeling, stimulus response experiments, continuous cultivation, Enzyme assay, Mass spectrometry (LC-MS/MS), HPLC, GC and LC/MS analysis of metabolites, ODE, Parameter estimation
I've become a SysMO DB PAL for MOSES project in 2007 being a post-doc in lab of Prof. Matthias Reuss at University of Stuttgart. In the MOSES project, our major efforts were in the experimental data acquisition for dynamic model of primary carbon and anaerobic energy metabolism in yeast. The model implements prediction of perturbations of two types: glucose pulse and temperature jump. We implement “stimulus-response” methodology for the unraveling the dynamic structure of the network and to ...
SysMO is a European transnational funding and research initiative on "Systems Biology of Microorganisms".
The goal pursued by SysMO was to record and describe the dynamic molecular processes going on in unicellular microorganisms in a comprehensive way and to present these processes in the form of computerized mathematical models.
Systems biology will raise biomedical and biotechnological research to a new quality level and contribute markedly to progress in understanding. Pooling European research ...
Projects: BaCell-SysMO, COSMIC, SUMO, KOSMOBAC, SysMO-LAB, PSYSMO, SCaRAB, MOSES, TRANSLUCENT, STREAM, SulfoSys, SysMO DB, SysMO Funders, SilicoTryp, Noisy-Strep
Web page: http://sysmo.net/
MOSES (Micro Organism Systems biology: Energy and Saccharomyces cerevisiae) develops a new Systems Biology approach, which is called 'domino systems biology'. It uses this to unravel the role of cellular free energy ('ATP') in the control and regulation of cell function. MOSES operates though continuous iterations between partner groups through a new systems-biology driven data-management workflow. MOSES also tries to serve as a substrate for three or more other SYSMO programs.
Programme: SysMO
Public web page: http://www.moses.sys-bio.net/
Organisms: Saccharomyces cerevisiae
Steady state metabolic fluxes and metabolite concentrations of yeast Saccharomyces cerevisiae in anaerobic chemostat at D=0.1 h-1
Submitter: Maksim Zakhartsev
Studies: Steady state concentrations of metabolites in yeast Saccharomyces cerevi..., Steady state fluxes in yeast Saccharomyces cerevisiae in anaerobic chemo...
Assays: Steady state concentrations of extracellular metabolites in yeast Saccha..., Steady state concentrations of intracellular metabolites in yeast Saccha..., Steady state extracellular fluxes in anaerobic yeast Saccharomyces cerev...
Snapshots: No snapshots
Steady state fluxes in yeast Saccharomyces cerevisiae in anaerobic chemostat at D=0.1 h-1
Submitter: Maksim Zakhartsev
Investigation: Steady state metabolic fluxes and metabolite co...
Assays: Steady state extracellular fluxes in anaerobic yeast Saccharomyces cerev...
Snapshots: No snapshots
Steady state metabolic fluxes measured in glucose-limited chemostat of Saccharomyces cerevisiae at D = 0.1 h-1 growing on minimal medium. Fluxes are: glucose, ethanol, glycerol, acetate, succinate, pyruvate, lactate, citrate, malate, a-ketoglutarate, fumarate
Investigations: Kinetic analysis of metabolic system using tran..., Steady state metabolic fluxes and metabolite co...
Studies: Metabolic perturbation of the steady state cult..., Steady state fluxes in yeast Saccharomyces cere...
Assays: MOSES: dynamic model of glucose pulse, Steady state extracellular fluxes in anaerobic ...
SOP for growing yeast in anaerobic conditions
Creator: Maksim Zakhartsev
Submitter: Maksim Zakhartsev
Investigations: Kinetic analysis of metabolic system using tran..., Steady state metabolic fluxes and metabolite co...
Studies: Metabolic perturbation of the steady state cult..., Steady state concentrations of metabolites in y..., Steady state fluxes in yeast Saccharomyces cere...
Assays: Biomass weight during glucose pulse, Cellular size and granularity during glucose pulse, Dynamics of extracellular metabolites during gl..., Dynamics of intracellular metabolites during gl..., Dynamics of macromolecules during glucose pulse, Steady state concentrations of extracellular me..., Steady state concentrations of intracellular me..., Steady state extracellular fluxes in anaerobic ...
Biomass sampling - SOP for sampling of biomass
Creator: Maksim Zakhartsev
Submitter: Maksim Zakhartsev
Investigations: Kinetic analysis of metabolic system using tran..., Steady state metabolic fluxes and metabolite co...
Studies: Metabolic perturbation of the steady state cult..., Steady state concentrations of metabolites in y..., Steady state fluxes in yeast Saccharomyces cere...
Assays: Biomass weight during glucose pulse, Cellular size and granularity during glucose pulse, Dynamics of extracellular metabolites during gl..., Dynamics of intracellular metabolites during gl..., Dynamics of macromolecules during glucose pulse, Steady state concentrations of extracellular me..., Steady state concentrations of intracellular me..., Steady state extracellular fluxes in anaerobic ...
Yeast strains used in the project
Creator: Maksim Zakhartsev
Submitter: Maksim Zakhartsev
Investigations: Kinetic analysis of metabolic system using tran..., Steady state metabolic fluxes and metabolite co...
Studies: Metabolic perturbation of the steady state cult..., Steady state concentrations of metabolites in y..., Steady state fluxes in yeast Saccharomyces cere...
Assays: Biomass weight during glucose pulse, Cellular size and granularity during glucose pulse, Dynamics of extracellular metabolites during gl..., Dynamics of intracellular metabolites during gl..., Dynamics of macromolecules during glucose pulse, Steady state concentrations of extracellular me..., Steady state concentrations of intracellular me..., Steady state extracellular fluxes in anaerobic ...
Abstract (Expand)
Authors: Maksim Zakhartsev, Oliver Vielhauer, Thomas Horn, Xuelian Yang, Matthias Reuss
Date Published: 1st Apr 2015
Publication Type: Not specified
DOI: 10.1007/s11306-014-0700-8
Citation: Metabolomics 11(2) : 286