The FAIRDOMHub

13 items tagged with 'chemostat'.

Steady state extracellular fluxes in anaerobic yeast Saccharomyces cerevisiae

MOSES

experimentally measured extracellular fluxes in yeast Saccharomyces cerevisiae in anaerobic glucose limited chemostat (D=0.1 h-1) on minimal medium

Contributor: Maksim Zakhartsev

Assay type: Metabolite profiling

Technology type: Hplc

Investigation: Steady state metabolic fluxes and metabolite co...

Study: Steady state fluxes in yeast Saccharomyces cere...

Organisms: Saccharomyces cerevisiae (chemostat)

SOPs: Anaerobic media composition, Sampling of biomass, Yeast strains

Data files: Measured steady state metabolic fluxes

Created: 26th Apr 2010 at 16:14, Last updated: 27th Mar 2012 at 11:44

Fermentation G

COSMIC

acetic acid pulse 100mM
acetic acid step 50mM, 70mM

Creator: Sebastian Curth

Contributor: Sebastian Curth

Created: 11th Dec 2012 at 15:14

Fermentation F

COSMIC

acetic acid addition

step experiment:
50mM, 70mM, 100mM, 120mM

pulse
200mM

Creator: Sebastian Curth

Contributor: Sebastian Curth

Created: 11th Dec 2012 at 14:37

Fermentation E

COSMIC

pulse acetic acid: 70mM (2x), 80mM,
shift to 4.5,
pulse acetic acid: 70mM

Creator: Sebastian Curth

Contributor: Sebastian Curth

Created: 11th Dec 2012 at 14:35

Fermentation D

COSMIC

acetic acid addition 70mM

Creator: Sebastian Curth

Contributor: Sebastian Curth

Created: 11th Dec 2012 at 14:32

Steady state transcriptional profiles at different oxygen concentrations

SUMO

Transcriptional profiles of steady-state chemostat cultures were measured at a range of oxygen levels using microarrays. Oxygen levels were defined by the aerobiosis scale of Alexeeva et al. (2002).

Creator: Matthew Rolfe

Contributor: Matthew Rolfe

Created: 16th Mar 2010 at 18:13, Last updated: 15th Jan 2013 at 13:44

Measured steady state concentrations of intracellular metabolites in yeast Saccharomyces cerevisiae anaerobic chemostat

MOSES

Steady state concentrations of intracellular metabolites in yeast Saccharomyces cerevisiae anaerobic chemostat at D = 0.1 h-1 on minimal medium. All metabolite concentrations are in mmol/L(CV).

Creator: Maksim Zakhartsev

Contributor: Maksim Zakhartsev

Created: 27th Apr 2010 at 11:42, Last updated: 30th Jan 2012 at 10:51

Reprogramming of Escherichia coli K-12 metabolism during the initial phase of transition from an anaerobic to a micro-aerobic environment

SUMO

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Abstract (Expand)

Many bacteria undergo transitions between environments with differing O₂ availabilities as part of their natural lifestyles and during biotechnological processes. However, the dynamics of adaptation ...

Authors: Matthew Rolfe, Guido Sanguinetti, Robert Poole, Jeff Green, Trotter Eleanor W,Hounslow Andrea M,Craven C Jeremy,Williamson Michael P

Date Published: 27th Sep 2011

Journal: PLoS ONE

PubMed ID: 21980479

Citation:

Created: 29th Aug 2012 at 15:50, Last updated: 29th Aug 2012 at 15:50

Systems analysis of transcription factor activities in environments with stable and dynamic oxygen concentrations

SUMO

(Show All)

Abstract (Expand)

Understanding gene regulation requires knowledge of changes in transcription factor (TF) activities. Simultaneous direct measurement of numerous TF activities is currently impossible. Nevertheless, ...

Authors: Matthew Rolfe, Robert Poole, Guido Sanguinetti, Jeff Green, Ocone Andrea,Stapleton Melanie R,Hall Simon,Trotter Eleanor W

Date Published: 8th Aug 2012

Journal: Open Biol

PubMed ID: 22870390

Citation:

Created: 29th Aug 2012 at 15:36, Last updated: 29th Aug 2012 at 15:36

Simplified absolute metabolite quantification by gas chromatography–isotope dilution mass spectrometry on the basis of commercially available source material

MOSES

Abstract (Expand)

In the field of metabolomics, GC–MS has rather established itself as a tool for semi-quantitative strategies like metabolic fingerprinting or metabolic profiling. Absolute quantification of intra- or ...

Authors: Maksim Zakhartsev, Matthias Reuss, Vielhauer Oliver,Horn Thomas,Takors Ralf

Date Published: 1st Dec 2011

Journal: Journal of Chromatography B

DOI: 10.1016/j.jchromb.2011.10.036

Citation:

Created: 1st Mar 2012 at 12:04, Last updated: 1st Mar 2012 at 12:05

Berlin Progress Report

COSMIC

No description specified

Creator: Sebastian Curth

Contributor: Sebastian Curth

Created: 5th Apr 2012 at 16:16

Chemostat 200109_s0001

Training material

Creators: Olga Krebs, Maksim Zakhartsev

Contributor: Olga Krebs

Created: 24th Feb 2017 at 11:47, Last updated: 24th Feb 2017 at 11:47

YEAST_chemostat_steady_state_culture

FAIRDOM

SAMPLE TYPE to describe samples of CEN.PK-113-7D yeast strain upon metabolic perturbation of the steady state culture by glucose pulse

Attributes: SEEK ID (Integer) *, STRAIN (SEEK Strain) , Genotype (String) , Cell culture ID (String) , Cell culture start date (Date) , Glucose pulse (concentration) (Integer) ( g/L ) , Sampling date (Date) , Time after glucose pulse (Integer) ( s ) , Cell part (Controlled Vocabulary) , Age at sampling (Integer) ( h ) *

Tags: yeast, genotype, chemostat, glucose pulse, Metabolomics

Created: 23rd Feb 2017 at 18:45