Systems analysis of transcription factor activities in environments with stable and dynamic oxygen concentrations

Abstract:

Understanding gene regulation requires knowledge of changes in transcription factor (TF) activities. Simultaneous direct measurement of numerous TF activities is currently impossible. Nevertheless, statistical approaches to infer TF activities have yielded non-trivial and verifiable predictions for individual TFs. Here, global statistical modelling identifies changes in TF activities from transcript profiles of Escherichia coli growing in stable (fixed oxygen availabilities) and dynamic (changing oxygen availability) environments. A core oxygen-responsive TF network, supplemented by additional TFs acting under specific conditions, was identified. The activities of the cytoplasmic oxygen-responsive TF, FNR, and the membrane-bound terminal oxidases implied that, even on the scale of the bacterial cell, spatial effects significantly influence oxygen-sensing. Several transcripts exhibited asymmetrical patterns of abundance in aerobic to anaerobic and anaerobic to aerobic transitions. One of these transcripts, ndh, encodes a major component of the aerobic respiratory chain and is regulated by oxygen-responsive TFs ArcA and FNR. Kinetic modelling indicated that ArcA and FNR behaviour could not explain the ndh transcript profile, leading to the identification of another TF, PdhR, as the source of the asymmetry. Thus, this approach illustrates how systematic examination of regulatory responses in stable and dynamic environments yields new mechanistic insights into adaptive processes.

SEEK ID: https://fairdomhub.org/publications/177

PubMed ID: 22870390

Projects: SUMO

Journal: Open Biol

Citation:

Date Published: 8th Aug 2012

Authors: Matthew Rolfe, Andrea Ocone, Melanie R Stapleton, Simon Hall, Eleanor W Trotter, Robert Poole, Guido Sanguinetti, Jeff Green

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Created: 29th Aug 2012 at 15:36

Last updated: 29th Aug 2012 at 15:36

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