Recent studies revealed the unsuspected complexity of the bacterial transcriptome but its systematic analysis across many diverse conditions remains a challenge. Here we report the condition-dependent transcriptome of the prototype strain B. subtilis 168 across 104 conditions reflecting the bacterium's life-styles. This data set composed of 269 tiling array hybridizations allowed to observe ~85% of the annotated CDSs expressed in the higher 30% in at least one hybridization and thus provide an ...
Creators: Leif Steil, Ulrike Mäder, Colin Harwood, Rick Lewis, Ruben Mars, Priyanka Nannapaneni, Praveen kumar Sappa, Joerg Stuelke, Jan Maarten van Dijl, Michael Hecker, Uwe Voelker
Submitter: Leif Steil
Investigations: Redefining the Complete Transcriptome of Bacill...
Studies: Transcriptome analysis of glucose starvation in..., Transcriptome of continuously stressed B. subtilis, Transcriptome of shocked B. subtilis cells
Assays: Tiling Array analysis of glucose strarved B. su..., Tiling Array analysis of shocked B. subtilis cells, Tiling array analysis of continuous growth stre...
Transcriptional profiles of steady-state chemostat cultures were measured at a range of oxygen levels using microarrays. Oxygen levels were defined by the aerobiosis scale of Alexeeva et al. (2002).
Creator: Matthew Rolfe
Submitter: Matthew Rolfe
This Excel template is for use with affy transcriptomics data in Matrix table format. It was created from a template on the GEO web site (http://www.ncbi.nlm.nih.gov/geo/info/geo_affy.html) and modified to conform to the SysMO JERM for transcriptomics.
Creator: Katy Wolstencroft
Submitter: Katy Wolstencroft
Investigations: Creating data sheet template for 'omics data
Studies: Creating Templates for Transcriptomics
Assays: Affy Transcriptomics Templates
Standard Operating Procedure describes the labeling of eukaryotic RNA with aminoallyl labeled nucleotides via first strand cDNA synthesis followed by a coupling of the aminoallyl groups to either Cyanine 3 or 5 (Cy 3/Cy5) fluorescent molecules (published by The Institute For Genomic Research
Creators: Olga Krebs, Jeremy Hasseman , Emily Chen, Ivana Yang
Submitter: Olga Krebs
Investigations: No Investigations
Studies: No Studies
Assays: No Assays
The procedure describes the preparation of fluorescent DNA probes from human mRNA or total RNA.
Creator: Olga Krebs
Submitter: Olga Krebs
Investigations: No Investigations
Studies: No Studies
Assays: No Assays
Abstract (Expand)
Authors: Eleanor W Trotter, , Andrea M Hounslow, C Jeremy Craven, Michael P Williamson, , ,
Date Published: 27th Sep 2011
Publication Type: Not specified
PubMed ID: 21980479
Citation:
Abstract (Expand)
Authors: , Andrea Ocone, Melanie R Stapleton, Simon Hall, Eleanor W Trotter, , ,
Date Published: 8th Aug 2012
Publication Type: Not specified
PubMed ID: 22870390
Citation: