K-12/MG1655
Not specified
Not specified
Not specified
Not specified
wild-type
wild-type
Views: 3062
Created: 28th Apr 2010 at 16:21
Last updated: 19th Oct 2012 at 15:57
This item has not yet been tagged.
Related items
Projects: SUMO
Institutions: Max Planck Institute for Dynamics of Complex Technical Systems
Expertise: Microbiology, Molecular Biology, E. coli, Biotechnology
Tools: qRT-PCR, Bioreactor experiments/Fermentation (batch, Chemostat), Enzyme assays, 2D-PAGE
These files show physiological measurements from the Sheffield Infors chemostat which were made during acetate calibration and also when sampling for the steady-state transcriptional profiles.
Submitter: Matthew Rolfe
Assay type: Experimental Assay Type
Technology type: Technology Type
Investigation: Steady state studies for different oxygen avail...
Organisms: Escherichia coli : K-12/MG1655 (wild-type / wild-type)
SOPs: DCW measurement, Evans Medium, SUMO chemostat conditions
Data files: Sheffield chemostat parameters .csv, Sheffield chemostat parameters .pdf
Snapshots: No snapshots
This assay involved the determination of transcriptional profiles at 0, 2, 5, 10, 15 and 20 minutes through aerobic to anaerobic gas transitions and anaerobic to aerobic gas transitions. In each case an aerobic or anaerobic steady state was created, RNA sampled (0 min) and then the gas supply changed. RNA samples were then taken from the time at which the gas supply was changed.
For anaerobic conditions 5% CO2, 95% N2 was used.
The full transcriptional dataset is available from ArrayExpress ...
Submitter: Matthew Rolfe
Assay type: Transcriptional Profiling
Technology type: Microarray
Investigation: Dynamical studies for different oxygen availabi...
Organisms: Escherichia coli : K-12/MG1655 (wild-type / wild-type)
SOPs: Evans Medium, SUMO RNA isolation from E. coli, SUMO Transcriptional profiling of E. coli, SUMO chemostat conditions
Data files: Dissolved oxygen tension aerobic to anaerobic .pdf, Dissolved oxygen tension anaerobic to aerobic .pdf, Entire transcriptional dataset - aerobic to ana..., Entire transcriptional dataset - anaerobic to a..., Filtered dataset - anaerobic to aerobic .csv, Filtered datset - aerobic to anaerobic transiti...
Snapshots: No snapshots
The transcriptional profiles of steady state E. coli cultures at a range of aerobiosis levels were determined. Two biological replicates and two technical replicates were carried out. Microarrays were carried out in a reference style (i.e. RNA vs a gDNA reference).
Submitter: Matthew Rolfe
Assay type: Transcriptomics
Technology type: Microarray
Investigation: Steady state studies for different oxygen avail...
Organisms: Escherichia coli : K-12/MG1655 (wild-type / wild-type)
SOPs: SUMO RNA isolation from E. coli, SUMO Transcriptional profiling of E. coli
Data files: Steady state transcriptional profiles at differ...
Snapshots: No snapshots
This assay describes the determination of concentrations and ratio of metabolites of adenine nucleotides (NAD and NADH). These metabolites have been extracted from Escherichia coli MG1655 and isgenic mutant strains.
Submitter: Stefan Stagge
Assay type: Metabolomics
Technology type: Initial Rate Experiment
Investigation: Steady state studies for different oxygen avail...
Organisms: Escherichia coli : K-12/MG1655 (wild-type / wild-type)
SOPs: 1 hidden item
Data files: 2 hidden items
Snapshots: No snapshots
This assay describes the determination of concentrations and ratio of metabolites of ubiquinones (oxidised and reduced form). These metabolites have been extracted from Escherichia coli MG1655 and isgenic mutant strains.
Submitter: Stefan Stagge
Assay type: Metabolomics
Technology type: HPLC
Investigation: Steady state studies for different oxygen avail...
Organisms: Escherichia coli : K-12/MG1655 (wild-type / wild-type)
SOPs: DCW measurement, Q/QH2 sampling, SUMO chemostat conditions
Data files: 091016 quinones complete data magdeburg, 091016 quinones complete data magdeburg
Snapshots: No snapshots
This .csv file shows the numbers of different cytochrome molecules per cell from steady-state continuously-grown cultures at various aerobiosis levels (0%, 31%, 56%, 85% and 115% AAU).
Submitter: Alison Graham
Assay type: Experimental Assay Type
Technology type: Technology Type
Investigation: Steady state studies for different oxygen avail...
Organisms: Escherichia coli : K-12/MG1655 (wild-type / wild-type)
SOPs: No SOPs
Data files: No Data files
Snapshots: No snapshots
E. coli MG1655 and ∆sdhC and ∆frdA isogenic mutant strains were characterized in batch growth curves aerobic and anaerobically. Optical density, glucose consumption and by-product accumulation were measured during growth.
Submitter: Sonja Steinsiek
Assay type: Experimental Assay Type
Technology type: Technology Type
Investigation: Steady state studies for different oxygen avail...
Organisms: Escherichia coli : K-12/MG1655 (wild-type / wild-type)
SOPs: Evans Medium, Measurement of extracellular metabolites
Data files: 2 hidden items
Snapshots: No snapshots