mRNA decay assay

This assay is designed to measure the decay kinetics of mRNA in T. brucei blood forms. T. brucei lacks the canonical transcriptional regulation employed by other eukaryotes through transcription factors, and relies almost entirely on regulation of mRNA decay and further downstream steps in order to control gene expression.
3 replicates of 8 time points were taken to measure mRNA abundance in the cell using RNA-seq. The first time point was fot WT, untreated cells; the second was 5 min after the addition of the splicing inhibitor Sinefungin; the following time points were after inhibition of transcription with Actinomycin D.

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Created: 21st Mar 2012 at 20:09

Last updated: 8th Nov 2017 at 15:21

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