Generation of uniformly 13C-labelled E. coli extract for absolute quantification Version 1
Labelling and extraction procedure for uniformly 13C-labelled E. coli (MG1655) for absolute quantification using isotope dilution technique by LC-MS
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SOP for labelled E coli extraction.docx
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Created: 27th Feb 2012 at 12:00
Last updated: 11th Jan 2013 at 16:11
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Version 1 (earliest) Created 27th Feb 2012 at 12:00 by Dong-Hyun Kim
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Projects: SilicoTryp
Institutions: University of Glasgow
SysMO is a European transnational funding and research initiative on "Systems Biology of Microorganisms".
The goal pursued by SysMO was to record and describe the dynamic molecular processes going on in unicellular microorganisms in a comprehensive way and to present these processes in the form of computerized mathematical models.
Systems biology will raise biomedical and biotechnological research to a new quality level and contribute markedly to progress in understanding. Pooling European research ...
Projects: BaCell-SysMO, COSMIC, SUMO, KOSMOBAC, SysMO-LAB, PSYSMO, SCaRAB, MOSES, TRANSLUCENT, STREAM, SulfoSys, SysMO DB, SysMO Funders, SilicoTryp, Noisy-Strep
Web page: http://sysmo.net/
The SilicoTryp project aims at the creation of a “Silicon Trypanosome”, a comprehensive, experiment-based, multi-scale mathematical model of trypanosome physiology. Trypanosomes are blood-stream parasites transmitted by tsetse flies; they cause African sleeping sickness in humans and livestock. Currently available drugs have severe side effects, and the parasites are rapidly developing resistance. In this project, we collect a wide range of new experimental data on the parasite in its various ...
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Public web page: http://silicotryp.ibls.gla.ac.uk/wiki/Main_Page
The aims of this investigation is to quantify metabolites associated with pathways involved in stress responses for parameterising models of oxidative stress metabolism; the measurement of metabolic fluxes of metabolites of interest with intracellular concentrations
Submitter: Dong-Hyun Kim
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Assays: Generation of uniformly 13C-labelled E. coli extract, Intracellular metabolite concentrations in T. brucei exposed to oxidativ..., Intracellular metabolite concentrations in T. brucei under pH stress, LC-MS based absolute quantification of extracellular metabolites, LC-MS based absolute quantification of intracellular metabolites, Metabolite profiling on T. brucei exposed to oxidative stress
Flux will be measured using the metabolomics platforms based on absolute quantification method (isotope ratio based MS technique) by LC-MS, using heavy-isotope labelled precursors of the metabolites of interest. For example, 15N labelled cysteine, glycine and glutamate will be used to determine rates of synthesis of glutathione. 15N-labelled methionine to measure S-adenosyl methionine (and its decarboxylated form, as well as methionine cycle intermediates). 15N labelled arginine is used as precursor ...
This assay is for method development to quantify intra- and extra-cellular metabolites on T. brucei 427 bloodstream form using isotope ratio based MS technique with 13C-labelled E. coli extract
Submitter: Dong-Hyun Kim
Assay type: Metabolite Concentration
Technology type: Mass Spectrometry
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Study: Metabolic flux measurement
