Compare ATP extraction methods
We will compare two different procedures to extract ATP from yeast cells: Standard kit procedure (hot Tris/EDTA) and Serrano procedure (cold perchloric acid). In addition we have tested different condition as it turned out that some are important.
SEEK ID: https://fairdomhub.org/assays/102
Experimental assay
Projects: MOSES
Investigation: Effect of Benzoic Acid on ATP Levels
Study: Test different ATP extraction protocols and conditions
Assay position:
Assay type: Metabolomics
Technology type: Imaging
Organisms: Saccharomyces cerevisiae : YSBN2 diploid (wild-type / wild-type)
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Created: 8th Jun 2010 at 03:50
Last updated: 8th Nov 2017 at 14:21
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Projects: MOSES
Institutions: Medical University Vienna
General Bioinformatics
SysMO is a European transnational funding and research initiative on "Systems Biology of Microorganisms".
The goal pursued by SysMO was to record and describe the dynamic molecular processes going on in unicellular microorganisms in a comprehensive way and to present these processes in the form of computerized mathematical models.
Systems biology will raise biomedical and biotechnological research to a new quality level and contribute markedly to progress in understanding. Pooling European research ...
Projects: BaCell-SysMO, COSMIC, SUMO, KOSMOBAC, SysMO-LAB, PSYSMO, SCaRAB, MOSES, TRANSLUCENT, STREAM, SulfoSys, SysMO DB, SysMO Funders, SilicoTryp, Noisy-Strep
Web page: http://sysmo.net/
MOSES (Micro Organism Systems biology: Energy and Saccharomyces cerevisiae) develops a new Systems Biology approach, which is called 'domino systems biology'. It uses this to unravel the role of cellular free energy ('ATP') in the control and regulation of cell function. MOSES operates though continuous iterations between partner groups through a new systems-biology driven data-management workflow. MOSES also tries to serve as a substrate for three or more other SYSMO programs.
Programme: SysMO
Public web page: http://www.moses.sys-bio.net/
This investigation examines the effect of benzoic acid, a food preservative, on the ATP levels of yeast cells.
Submitter: Martin Valachovic
Studies: ATP levels after benzoic acid pretreatments, ATP levels under 10 mM benzoic acid stress, ATP levels under 30 mM and 50 mM benzoic acid stress, Cell survival under different benzoic acid concentrations, Test different ATP extraction protocols and conditions
Assays: ATP measurement under 10mM benzoic acid stress, ATP measurement under 30mM benzoic acid stress, ATP measurement under 50mM benzoic acid stress, Compare ATP extraction methods
This study aims to establish the optimum conditions and assay methods for measuring ATP levels
Submitter: Martin Valachovic
Investigation: Effect of Benzoic Acid on ATP Levels
Assays: Compare ATP extraction methods
Submitter: Walter Glaser
Provider Name: Not specified
Provider's strain ID: Not specified
Organism: Saccharomyces cerevisiae
Genotypes: wild-type
Phenotypes: wild-type
Comment: Not specified
The kit is used for the quantitative detection of ATP in yeast cells by luciferase driven bioluminescence. ATP extraction is done by boiling cells in TE buffer.
Creators: Martin Valachovic, Cornelia Klein
Submitter: Walter Glaser
