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SEEK ID: https://fairdomhub.org/people/89
Location:
Austria
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MOSES (Micro Organism Systems biology: Energy and Saccharomyces cerevisiae) develops a new Systems Biology approach, which is called 'domino systems biology'. It uses this to unravel the role of cellular free energy ('ATP') in the control and regulation of cell function. MOSES operates though continuous iterations between partner groups through a new systems-biology driven data-management workflow. MOSES also tries to serve as a substrate for three or more other SYSMO programs.
Programme: SysMO
Public web page: http://www.moses.sys-bio.net/
Organisms: Saccharomyces cerevisiae
This investigation examines the effect of benzoic acid, a food preservative, on the ATP levels of yeast cells.
Snapshots: No snapshots
Studies: ATP levels after benzoic acid pretreatments, ATP levels under 10 mM benzoic acid stress, ATP levels under 30 mM and 50 mM benzoic acid stress, Cell survival under different benzoic acid concentrations, Test different ATP extraction protocols and conditions
Assays: ATP measurement under 10mM benzoic acid stress, ATP measurement under 30mM benzoic acid stress, ATP measurement under 50mM benzoic acid stress, Compare ATP extraction methods
Cell survival was determined under different benzoic acid concentrations
Person responsible: Martin Valachovic
Snapshots: No snapshots
Investigation: Effect of Benzoic Acid on ATP Levels
Assays: No Assays
Effect of benzoate treatment (high concentrations) on ATP levels and Pdr12 expression after pretreatment of cells with low concentrations of benzoic acid.
Person responsible: Martin Valachovic
Snapshots: No snapshots
Investigation: Effect of Benzoic Acid on ATP Levels
Assays: No Assays
ATP levels of cells stressed with higher concentrations of benzoic acid (30 mM and 50 mM).
Person responsible: Martin Valachovic
Snapshots: No snapshots
To see changes in ATP levels in cells with induced ABC transporters. Cells with Pdr12 pump by 10 mM benzoic acid are used.
Person responsible: Martin Valachovic
Snapshots: No snapshots
Investigation: Effect of Benzoic Acid on ATP Levels
This study aims to establish the optimum conditions and assay methods for measuring ATP levels
Person responsible: Martin Valachovic
Snapshots: No snapshots
Investigation: Effect of Benzoic Acid on ATP Levels
Assays: Compare ATP extraction methods
The kit is used for the quantitative detection of ATP in yeast cells by luciferase driven
bioluminescence. ATP extraction is done by boiling cells in TE buffer.
Creators: Martin Valachovic, Cornelia Klein
Contributor: Walter Glaser
Investigations: Effect of Benzoic Acid on ATP Levels
Studies: ATP levels under 10 mM benzoic acid stress, ATP levels under 30 mM and 50 mM benzoic acid s..., Test different ATP extraction protocols and con...
Assays: ATP measurement under 10mM benzoic acid stress, ATP measurement under 30mM benzoic acid stress, ATP measurement under 50mM benzoic acid stress, Compare ATP extraction methods