SOPs

Created At
Go
230 SOPs visible to you, out of a total of 449

Protocol for transfer of plasmids into Clostridium spp. by conjugation

Creators: Ying Zhang, Nigel Minton

Submitter: Ying Zhang

A refined and streamlined procedure to generate mutant in a wide range of different clostridial species, using group II intron retargeting methodologies.

Creators: Ying Zhang, Nigel Minton

Submitter: Ying Zhang

This protocol describes the transcriptional profiling of E. coli cultures using microarrays. The protocol utilises RNA isolated as described in another SOP (SUMO RNA isolation from E. coli) and with hybridisation to Ocimum Ocichip E. coli K-12 microarrays.

No description specified

Creator: Michael Kohlstedt

Submitter: Michael Kohlstedt

General protocol for measuring the kinetic parameters of the purified glycolytic enzymes from Saccharomyces cerevisiae - SOP for measuring the kinetic parameters of the purified glycolytic isoenzymes

Creator: Hanan Messiha

Submitter: Maksim Zakhartsev

Sample preparation - SOP for sampling, preparation of cell-free extracts, and determination of total extracted protein

Creator: Femke Mensonides

Submitter: Maksim Zakhartsev

We routinely select specific RNAi gene targets (400–600 bp) and primers using the RNAit software http://trypanofan.path.cam.ac.uk/software/RNAit.html. A single pair of PCR primers are designed that incorporate four selected restriction sites (not present in the RNAi target fragment) such that a single PCR product can be differentially digested and sequentially cloned. For example, using MCS1/2, the following primers could be used to clone antisense followed by sense fragments: Primer 1, XbaI–BamHI-5′ ...

SOP for extracellular metabolite measurment

Written Standard Operating Procedures provide workers with the operational information necessary to perform a job properly and ensure consistency in the operations. Standard Operating Procedures provide a historical record of steps in the how, why and when and serve as a training tool for teaching users.

Creator: Olga Krebs

Submitter: Olga Krebs

No description specified
No description specified

Creator: Holger Janssen

Submitter: The JERM Harvester

No description specified
No description specified

Creators: Louise Thomas, Maggie Smith

Submitter: Jay Moore

No description specified

Creators: Louise Thomas, Maggie Smith

Submitter: Jay Moore

No description specified

Creators: Louise Thomas, Maggie Smith

Submitter: Jay Moore

No description specified

Creators: Louise Thomas, Maggie Smith

Submitter: Jay Moore

No description specified

Creators: Louise Thomas, Maggie Smith

Submitter: Jay Moore

No description specified
No description specified
No description specified
Powered by
(v.1.12.1)
Copyright © 2008 - 2022 The University of Manchester and HITS gGmbH

By continuing to use this site you agree to the use of cookies