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284 SOPs visible to you, out of a total of 522

SOP 7 Glucose-pulse experiments

SysMO-LAB

No description specified

Creator: Martijn Bekker

Submitter: The JERM Harvester

Investigations: Investigation of glycolysis and pyruvate branch..., The Attic

Studies: Comparative modeling and phosphate dependence f..., Pre-liminary data from Martijn Bekker

Assays: Glucose pulsed L. lactis, Kinetic behavior of intracellular metabolites o..., Kinetic behavior of intracellular metabolites o...

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Created: 26th Feb 2010 at 04:25, Last updated: 11th May 2016 at 17:20

Views: 4265, Downloads: 139

Analysis of organic acid by HPLC

SysMO-LAB

This HPLC method uses a isocratic method and a RI detector to identify and quantify almost all excreted catabolic metabolites.

Creator: Martijn Bekker

Submitter: Martijn Bekker

Investigations: Amino acid metabolism of four LAB species: Stre..., Investigation of glycolysis and pyruvate branch...

Studies: Arginine and Glutamine metabolism in S. pyogenes, Comparative modeling and phosphate dependence f...

Assays: Characterization of flux distribution of S. pyo..., Glucose pulsed S. pyogenes

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Created: 26th Oct 2009 at 15:48, Last updated: 11th May 2016 at 17:20

Views: 3862, Downloads: 131

Quenching of lactic acid bacteria

SysMO-LAB

A protocol for acidic quenching of lactic acid bacteria used for analyses of intracellular metabolites.

Creator: Martijn Bekker

Submitter: Martijn Bekker

Investigations: Investigation of glycolysis and pyruvate branch...

Studies: Comparative modeling and phosphate dependence f...

Assays: Glucose pulsed S. pyogenes

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Created: 26th Oct 2009 at 15:48, Last updated: 11th May 2016 at 17:20

Views: 3755, Downloads: 110

Protocol for glucose perturbations

SysMO-LAB

This protocol for applying glucose perturbations works for Lactococcus lactis and Enterococcus faecalis

Creator: Martijn Bekker

Submitter: Martijn Bekker

Investigations: Investigation of glycolysis and pyruvate branch...

Studies: Comparative modeling and phosphate dependence f...

Assays: Glucose pulsed S. pyogenes

Download

Created: 26th Oct 2009 at 15:48, Last updated: 11th May 2016 at 17:20

Views: 3817, Downloads: 88

SOP 8 Glucose-pulse experiments (adapted for S. pyogenes)

SysMO-LAB

No description specified

Creator: Martijn Bekker

Submitter: The JERM Harvester

Investigations: Investigation of glycolysis and pyruvate branch...

Studies: Comparative modeling and phosphate dependence f...

Assays: Glucose pulsed S. pyogenes

Download

Created: 26th Feb 2010 at 04:25, Last updated: 11th May 2016 at 17:20

Views: 3352, Downloads: 97

SOP 5 Analysis of intracellular metabolites

SysMO-LAB

No description specified

Creator: Martijn Bekker

Submitter: The JERM Harvester

Investigations: Investigation of glycolysis and pyruvate branch...

Studies: Comparative modeling and phosphate dependence f...

Assays: Glucose pulsed L. lactis

Download

Created: 26th Feb 2010 at 04:25, Last updated: 11th May 2016 at 17:20

Views: 3816, Downloads: 97

SOP 6 Analysis of intracellular metabolites

SysMO-LAB

No description specified

Creator: Martijn Bekker

Submitter: The JERM Harvester

Investigations: Investigation of glycolysis and pyruvate branch..., The Attic

Studies: Comparative modeling and phosphate dependence f..., Pre-liminary data from Martijn Bekker

Assays: Glucose pulsed S. pyogenes, Kinetic behavior of intracellular metabolites o..., Kinetic behavior of intracellular metabolites o...

Download

Created: 26th Feb 2010 at 04:25, Last updated: 11th May 2016 at 17:20

Views: 3840, Downloads: 117

SOP 2.1 Method for synthesis of LAB-medium used for the SYSMO-LAB project

SysMO-LAB

No description specified

Creator: Martijn Bekker

Submitter: The JERM Harvester

Investigations: Amino acid metabolism of four LAB species: Stre..., Investigation of glycolysis and pyruvate branch...

Studies: Arginine and Glutamine metabolism in S. pyogenes, Comparative modeling and phosphate dependence f...

Assays: Characterization of flux distribution of S. pyo..., Glucose pulsed L. lactis

Download

Created: 26th Feb 2010 at 04:25, Last updated: 11th May 2016 at 17:20

Views: 4073, Downloads: 94

RobOKoD SOP for Redesigning Butanol Producing Strain of E.coli.

Manchester Institute for Biotechnology

Matlab script that reproduces the model redesign results outlined in RobOKoD: microbial strain design for (over)production of target compounds (http://fairdomhub.org/publications/236).

Creator: Natalie Stanford

Submitter: Natalie Stanford

Investigations: Designing a new way to predict engineering stra...

Studies: Predicting engineering strategies for overprodu...

Assays: RobOKoD applied to e.coli for butanol production.

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Created: 3rd Jun 2015 at 14:16, Last updated: 8th Dec 2015 at 15:05

Views: 4564, Downloads: 142

OptKnock SOP for Redesigning Butanol Producing Strain of E.coli.

Manchester Institute for Biotechnology

Matlab script that reproduces the model redesign results outlined in RobOKoD: microbial strain design for (over)production of target compounds (http://fairdomhub.org/publications/236) for OptKnock.

Creator: Natalie Stanford

Submitter: Natalie Stanford

Investigations: Designing a new way to predict engineering stra...

Studies: Predicting engineering strategies for overprodu...

Assays: OptKnock applied to e.coli for butanol production.

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Created: 3rd Jun 2015 at 14:20, Last updated: 8th Dec 2015 at 15:05

Views: 4236, Downloads: 144

RobustKnock SOP for Redesigning Butanol Producing Strain of E.coli.

Manchester Institute for Biotechnology

Matlab script that reproduces the model redesign results outlined in RobOKoD: microbial strain design for (over)production of target compounds (http://fairdomhub.org/publications/236) for RobustKnock.

Creator: Natalie Stanford

Submitter: Natalie Stanford

Investigations: Designing a new way to predict engineering stra...

Studies: Predicting engineering strategies for overprodu...

Assays: RobustKnock applied to e.coli for butanol produ...

Download

Created: 3rd Jun 2015 at 14:27, Last updated: 8th Dec 2015 at 15:05

Views: 4489, Downloads: 146

Validation experiments

Whole body modelling of glucose metabolism in malaria patients

SOP for the determination of external metabolites (Glc, Pyr, Gly, Lac) in intact trophozoite incubations, and for the determination of intracellular metabolite concentrations.

Creators: Dawie van Niekerk, Jacky Snoep

Submitter: Dawie van Niekerk

Investigations: Glucose metabolism in Plasmodium falciparum tro...

Studies: Model validation

Assays: GLC incubation, Steady state

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Created: 15th Aug 2014 at 15:00, Last updated: 17th Apr 2015 at 12:27

Views: 4659, Downloads: 150

Culturing and synchronisation of P. falciparum

Whole body modelling of glucose metabolism in malaria patients

SOP for the cultivation conditions of Plasmodium falciparum, including the protocol for synchronisation.

Creators: Dawie van Niekerk, Jacky Snoep

Submitter: Dawie van Niekerk

Investigations: No Investigations

Studies: No Studies

Assays: No Assays

Download

Created: 15th Aug 2014 at 15:03, Last updated: 3rd Mar 2015 at 10:46

Views: 3434, Downloads: 104

G3PDH Kinetic characterisation

Whole body modelling of glucose metabolism in malaria patients

SOP for measurement of G3PDH activity in extracts.

Creators: Dawie van Niekerk, Jacky Snoep

Submitter: Dawie van Niekerk

Investigations: Glucose metabolism in Plasmodium falciparum tro...

Studies: Model construction

Assays: G3PDH

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Created: 27th Aug 2014 at 14:44, Last updated: 3rd Mar 2015 at 10:42

Views: 4583, Downloads: 113

ALD Kinetic characterisation

Whole body modelling of glucose metabolism in malaria patients

SOP for measurement of ALD activity in extracts.

Creators: Dawie van Niekerk, Jacky Snoep

Submitter: Dawie van Niekerk

Investigations: Glucose metabolism in Plasmodium falciparum tro...

Studies: Model construction

Assays: ALD

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Created: 15th Aug 2014 at 14:46, Last updated: 3rd Mar 2015 at 10:41

Views: 4291, Downloads: 117

GLCtr Kinetic characterisation

Whole body modelling of glucose metabolism in malaria patients

SOP for measurement of glucose transport activity in intact trophozoites.

Creators: Dawie van Niekerk, Jacky Snoep

Submitter: Dawie van Niekerk

Investigations: No Investigations

Studies: No Studies

Assays: No Assays

Download

Created: 15th Aug 2014 at 14:42, Last updated: 3rd Mar 2015 at 10:41

Views: 3528, Downloads: 103

GAPDH Kinetic characterisation

Whole body modelling of glucose metabolism in malaria patients

SOP for measurement of GAPDH activity in extracts.

Creators: Dawie van Niekerk, Jacky Snoep

Submitter: Dawie van Niekerk

Investigations: Glucose metabolism in Plasmodium falciparum tro...

Studies: Model construction

Assays: GAPDH

Download

Created: 15th Aug 2014 at 14:49, Last updated: 3rd Mar 2015 at 10:40

Views: 3964, Downloads: 118

ENO Kinetic characterisation

Whole body modelling of glucose metabolism in malaria patients

SOP for measurement of ENO activity in extracts.

Creators: Dawie van Niekerk, Jacky Snoep

Submitter: Dawie van Niekerk

Investigations: Glucose metabolism in Plasmodium falciparum tro...

Studies: Model construction

Assays: ENO

Download

Created: 15th Aug 2014 at 14:55, Last updated: 3rd Mar 2015 at 10:40

Views: 4163, Downloads: 114

HK Kinetic characterisation

Whole body modelling of glucose metabolism in malaria patients

SOP for measurement of HK activity in extracts.

Creators: Dawie van Niekerk, Jacky Snoep

Submitter: Dawie van Niekerk

Investigations: Glucose metabolism in Plasmodium falciparum tro...

Studies: Model construction

Assays: HK

Download

Created: 15th Aug 2014 at 14:43, Last updated: 3rd Mar 2015 at 10:40

Views: 3897, Downloads: 117

PFK Kinetic characterisation

Whole body modelling of glucose metabolism in malaria patients

SOP for measurement of PFK activity in extracts.

Creators: Dawie van Niekerk, Jacky Snoep

Submitter: Dawie van Niekerk

Investigations: Glucose metabolism in Plasmodium falciparum tro...

Studies: Model construction

Assays: PFK

Download

Created: 15th Aug 2014 at 14:45, Last updated: 3rd Mar 2015 at 10:39

Views: 4290, Downloads: 107

LDH Kinetic characterisation

Whole body modelling of glucose metabolism in malaria patients

SOP for measurement of LDH activity in extracts.

Creators: Dawie van Niekerk, Jacky Snoep

Submitter: Dawie van Niekerk

Investigations: Glucose metabolism in Plasmodium falciparum tro...

Studies: Model construction

Assays: LDH

Download

Created: 15th Aug 2014 at 14:57, Last updated: 3rd Mar 2015 at 10:39

Views: 4062, Downloads: 104

PGK Kinetic characterisation

Whole body modelling of glucose metabolism in malaria patients

SOP for measurement of PGK activity in extracts.

Creators: Dawie van Niekerk, Jacky Snoep

Submitter: Dawie van Niekerk

Investigations: Glucose metabolism in Plasmodium falciparum tro...

Studies: Model construction

Assays: PGK

Download

Created: 15th Aug 2014 at 14:52, Last updated: 3rd Mar 2015 at 10:38

Views: 3674, Downloads: 91

PK Kinetic characterisation

Whole body modelling of glucose metabolism in malaria patients

SOP for measurement of PK activity in extracts.

Creators: Dawie van Niekerk, Jacky Snoep

Submitter: Dawie van Niekerk

Investigations: Glucose metabolism in Plasmodium falciparum tro...

Studies: Model construction

Assays: PK

Download

Created: 15th Aug 2014 at 14:56, Last updated: 3rd Mar 2015 at 10:38

Views: 4000, Downloads: 98

PGM Kinetic characterisation

Whole body modelling of glucose metabolism in malaria patients

SOP for measurement of PGM activity in extracts.

Creators: Dawie van Niekerk, Jacky Snoep

Submitter: Dawie van Niekerk

Investigations: No Investigations

Studies: No Studies

Assays: No Assays

Download

Created: 27th Aug 2014 at 12:27, Last updated: 3rd Mar 2015 at 10:38

Views: 2885, Downloads: 83

PGI Kinetic characterisation

Whole body modelling of glucose metabolism in malaria patients

SOP for measurement of PGI activity in extracts.

Creators: Dawie van Niekerk, Jacky Snoep

Submitter: Dawie van Niekerk

Investigations: Glucose metabolism in Plasmodium falciparum tro...

Studies: Model construction

Assays: PGI

Download

Created: 15th Aug 2014 at 14:44, Last updated: 3rd Mar 2015 at 10:37

Views: 3479, Downloads: 97

PGM Kinetic characterisation

Whole body modelling of glucose metabolism in malaria patients

SOP for measurement of PGM activity in extracts.

Creators: Dawie van Niekerk, Jacky Snoep

Submitter: Dawie van Niekerk

Investigations: Glucose metabolism in Plasmodium falciparum tro...

Studies: Model construction

Assays: PGM

Download

Created: 15th Aug 2014 at 14:53, Last updated: 3rd Mar 2015 at 10:37

Views: 3579, Downloads: 89

TPI Kinetic characterisation

Whole body modelling of glucose metabolism in malaria patients

SOP for measurement of TPI activity in extracts.

Creators: Dawie van Niekerk, Jacky Snoep

Submitter: Dawie van Niekerk

Investigations: Glucose metabolism in Plasmodium falciparum tro...

Studies: Model construction

Assays: TPI

Download

Created: 15th Aug 2014 at 14:47, Last updated: 3rd Mar 2015 at 10:36

Views: 4314, Downloads: 95

Trophozoite Isolation and Lysate Preparation

Whole body modelling of glucose metabolism in malaria patients

SOP for the isolation of intact Plasmodium falciparum trophozoites from infected red blood cells and the preparation of a cell free extract that can be used for kinetic analyses.

Creators: Dawie van Niekerk, Jacky Snoep

Submitter: Dawie van Niekerk

Investigations: No Investigations

Studies: No Studies

Assays: No Assays

Download

Created: 15th Aug 2014 at 15:05, Last updated: 3rd Mar 2015 at 08:38

Views: 2866, Downloads: 85

SULFOSYSbiotech_Protein extraction_iTRAQ labeling_Peptides separation_mass spectrometry_data analysis

No description specified

Creator: Theresa Kouril

Submitter: Theresa Kouril

Investigations: No Investigations

Studies: No Studies

Assays: No Assays

Created: 5th Feb 2015 at 13:14

Views: 1973, Downloads: 0

Derivatization of Gsp and T(SH)2 produced in the trypanothione synthetase enzymatic assay

SilicoTryp

This method describes how to derivatize the N-glutathionylspermidine and trypanothione produced by T. brucei trypanothione synthetase under in vivo-like conditions

Creator: Alejandro Leroux

Submitter: Alejandro Leroux

Investigations: Kinetic understanding of the T. brucei trypanot...

Studies: Kinetic characterization of trypanothione-depen...

Assays: Trypanothione synthetase Gsp and T(SH)2 product...

Download

Created: 6th Aug 2014 at 16:04, Last updated: 6th Aug 2014 at 16:06

Views: 4312, Downloads: 141

(v.1.13.3)

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