SOPs

A Standard Operating Procedure (SOP) is a document consisting of step-by-step information on how to execute a task. An existing SOP may need to just be modified and updated.

Creator: Olga Krebs

Submitter: Deleted submitter

Investigations: Using SEEK and I-S-A structure to integrate ex...

Studies: Experimental study for hands on session

Assays: How to use templates for FAIR experimental data..., How to use templates to structure experimental ...

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Created: 27th May 2016 at 14:40, Last updated: 3rd Aug 2023 at 09:49

SOP_Preparation of cell lysates

Glucose metabolism in cancer cell lines

No description specified

Creator: Theresa Kouril

Submitter: Theresa Kouril

Investigations: Metabolic control analysis of glyceraldehyde 3-...

Studies: 3-Bromopyruvate (3BrP) titrations, Iodoacetic acid (IAA) titrations in cancer cell...

Assays: GAPDH and flux inhibition in MCF7 and MDA231cells, HK, GAPDH and flux inhibition in MDA231 cells, Incubation time for inhibitor treatment, Target verification/ Specificity of 3BrP in gly...

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Created: 13th Jul 2023 at 12:52

SOP_Inhibitor titration intact cells

Glucose metabolism in cancer cell lines

No description specified

Creator: Theresa Kouril

Submitter: Theresa Kouril

Investigations: Metabolic control analysis of glyceraldehyde 3-...

Studies: 3-Bromopyruvate (3BrP) titrations, Iodoacetic acid (IAA) titrations in cancer cell...

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Created: 13th Jul 2023 at 12:49

SOP_Quantification of glycolytic flux (glucose, lactate)

Glucose metabolism in cancer cell lines

No description specified

Creator: Theresa Kouril

Submitter: Theresa Kouril

Investigations: Metabolic control analysis of glyceraldehyde 3-...

Studies: 3-Bromopyruvate (3BrP) titrations, Iodoacetic acid (IAA) titrations in cancer cell...

Assays: GAPDH and flux inhibition in MCF7 and MDA231cells, HK, GAPDH and flux inhibition in MDA231 cells, Incubation time for inhibitor treatment

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Created: 12th Jul 2023 at 13:05

SOP_Cancer cell lines_standard cell culture

Glucose metabolism in cancer cell lines

No description specified

Creator: Theresa Kouril

Submitter: Theresa Kouril

Investigations: Metabolic control analysis of glyceraldehyde 3-...

Studies: 3-Bromopyruvate (3BrP) titrations, Iodoacetic acid (IAA) titrations in cancer cell...

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Created: 12th Jul 2023 at 13:03

Parameter Search SOP

PoLiMeR - Polymers in the Liver: Metabolism and Regulation

No description specified

Creators: Christoff Odendaal, Barbara Bakker

Submitter: Christoff Odendaal

Investigations: Mitochondrial fatty acid oxidation in human liver

Studies: Model construction

Assays: Kinetics Minireviews, Models

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Created: 28th Feb 2023 at 15:34, Last updated: 13th Jun 2023 at 14:53

P.SES-230405-V1_JP_Flow_Protocol.docx

Hi-IMPAcTB

No description specified

Creator: Charles Demurjian

Submitter: Charles Demurjian

Investigations: IMPAcTB

Studies: Specific CD4+ T cell phenotypes associate with ...

Assays: Flow Cytometry - Data Linked

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Created: 17th May 2023 at 20:44, Last updated: 17th May 2023 at 20:47

P.BOO-230324-V1_KC_Protocol

Hi-IMPAcTB

No description specified

Creator: Charles Demurjian

Submitter: Charles Demurjian

Investigations: IMPAcTB

Studies: Specific CD4+ T cell phenotypes associate with ...

Assays: Patient Visit - Metadata, Tissue Collection - Metadata

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Created: 17th May 2023 at 20:46, Last updated: 17th May 2023 at 20:47

P.KAM-230131-V1_cancer-cell-perfusion-and-extravasation-analysis.docx

MetNet

No description specified

Creator: Charles Demurjian

Submitter: Charles Demurjian

Investigations: MetNet

Studies: Impact of fibrinogen, fibrin thrombi and thromb...

Assays: Cancer Cell Extravasation Analysis - Data Linked

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Created: 16th May 2023 at 18:15, Last updated: 16th May 2023 at 18:27

P.KAM-230131-V1_clot-generate-and-image-analysis.docx

MetNet

No description specified

Creator: Charles Demurjian

Submitter: Charles Demurjian

Investigations: MetNet

Studies: Impact of fibrinogen, fibrin thrombi and thromb...

Assays: Clot Modeling Analysis - Data Linked

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Created: 16th May 2023 at 18:17, Last updated: 16th May 2023 at 18:27

P.KAM-230131-V1_OOC-protocol-device-and-network-creation.docx

MetNet

No description specified

Creator: Charles Demurjian

Submitter: Charles Demurjian

Investigations: MetNet

Studies: Impact of fibrinogen, fibrin thrombi and thromb...

Assays: Microfluidic Device Creation - Metadata

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Created: 16th May 2023 at 18:11, Last updated: 16th May 2023 at 18:25

P.KAM-230131-V1_Confocal-imaging.docx

MetNet

No description specified

Creator: Charles Demurjian

Submitter: Charles Demurjian

Investigations: MetNet

Studies: Impact of fibrinogen, fibrin thrombi and thromb...

Assays: Device Imaging - Data Linked, Permeability Analysis - Data Linked

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Created: 16th May 2023 at 18:14, Last updated: 16th May 2023 at 18:25

P.KAM-230131-V1_Flow.docx

MetNet

No description specified

Creator: Charles Demurjian

Submitter: Charles Demurjian

Investigations: MetNet

Studies: Impact of fibrinogen, fibrin thrombi and thromb...

Assays: Flow Cytometry - Data Linked, Flow Cytometry Analysis - Data Linked

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Created: 16th May 2023 at 18:18, Last updated: 16th May 2023 at 18:25

P.KAM-230131-V1_Cell-culture.docx

MetNet

No description specified

Creator: Charles Demurjian

Submitter: Charles Demurjian

Investigations: MetNet

Studies: Impact of fibrinogen, fibrin thrombi and thromb..., Patient-specific vascularized tumor model: Bloc..., Personalized Vascularized Models of Breast Canc..., Polyploidy of MDA-MB-231 cells drives increased..., Utilizing convolutional neural networks for dis...

Assays: Cell Culture - Metadata, Cell Culture and Tumor Spheroid Creation - Meta..., Cell Culture and Tumor Spheroid Creation - Meta..., Microfluidic Device Creation - Metadata, Tumoroid Formation - Metadata

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Created: 16th May 2023 at 18:10, Last updated: 16th May 2023 at 18:25

SOP for human genomic DNA extraction

MESI-STRAT

Standard Operating Procedure for human genomic DNA extraction ( copied from GEO DataSets GSM1375121)

Creators: Olga Krebs, Christiane Opitz

Submitter: Olga Krebs

Investigations: 1 hidden item

Studies: 1 hidden item

Assays: 1 hidden item

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Created: 19th Mar 2018 at 11:25, Last updated: 15th May 2023 at 12:58

SOP Induced fit docking

NMTrypI - New Medicines for Trypanosomatidic Infections

SOP describing application of the Induced fit docking workflow to Trypanosoma and Leishmania alpha-tubulin receptors.

Creators: Ina Poehner, Rebecca Wade

Submitter: Ina Poehner

Investigations: Antiparasitic dinitroaniline-ether phospholipid...

Studies: Docking studies of trifluraline and the dinitro...

Assays: Induced-fit docking studies

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Created: 21st Apr 2023 at 08:33, Last updated: 24th Apr 2023 at 13:36

SOP PIPSA

NMTrypI - New Medicines for Trypanosomatidic Infections

General SOP for the webPIPSA workflow to study electrostatic potential differences of full alpha-tubulin homology models and various putative binding site regions

Creators: Ina Poehner, Rebecca Wade

Submitter: Ina Poehner

Investigations: Antiparasitic dinitroaniline-ether phospholipid...

Studies: Computational identification of potential dinit...

Assays: Comparative electrostatic analysis of dinitroan...

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Created: 21st Feb 2023 at 11:43, Last updated: 24th Apr 2023 at 13:34

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