SEEK ID: https://fairdomhub.org/investigations/455
Projects: MIT SRP
Investigation position:
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Created: 29th Mar 2021 at 16:33
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Projects: Hi-IMPAcTB, MIT SRP
Institutions: Massachusetts Institute of Technology
Researcher at BioMIcro Center, Data Management and Analysis Core; Massachusetts Institute of Technology
Programme: MIT Superfund Research
Public web page: Not specified
Organisms: Not specified
Michelle Chen, Elliot I. Corless, Bevin P. Engelward, Timothy M. Swager
Link to Paper: https://pubs.acs.org/doi/full/10.1021/acsomega.4c03959
Agglutination of liquid Janus emulsions creates optical signals that can be detected and quantified. This study reports the detection and quantitation of Interleukin-6 (IL-6) using emulsions functionalized at the water-organic interface with engineered hyperthermophilic affinity proteins (rcSso7d) derived from ...
Submitter: Charles Demurjian
Investigation: MIT SRP
Assays: Agglutination Assay - Data Linked, All Metadata, Chemical Synthesis - Metadata, Fourier Transform Infrared Spectroscopy - Data Linked, Gel Permeation Chromatography - Data Linked, Nuclear Magnetic Resonance Spectroscopy - Data Linked, Nuclear Magnetic Resonance Spectroscopy Analysis - Data Linked, Pendant Drop Tensiometry - Data Linked
Snapshots: Snapshot 1
Jessica C. Beard, Chi-Hsien Wang, Arun Sridharan, Robert G. Croy, John M. Essigmann, Timothy M. Swager
Link to Paper: https://doi.org/10.1021/acssensors.4c00927
N-Nitrosamines are contaminants found throughout the environment, including in drinking water, and many nitrosamines are likely potent carcinogens. Correspondingly, there is need for rapid and cost-effective in-field detection methods that can provide timely information about their contamination ...
Submitter: Charles Demurjian
Investigation: MIT SRP
Assays: Chemical Synthesis - Metadata, Imaging - Data Linked, Nuclear Magnetic Resonance - Data Linked, Nuclear Magnetic Resonance Analysis - Data Linked, Single-crystal X-ray Crystallography - Data Linked, UV-Vis Spectroscopy - Data Linked, UV-Vis Spectroscopy Analysis - Data Linked
Snapshots: No snapshots
Jing Ge, Le P. Ngo, Simran Kaushal, Ian J. Tay, Elina Thadhani, Jennifer E. Kay, Patrizia Mazzucato, Danielle N. Chow, Jessica L. Fessler, David M. Weingeist, Robert W. Sobol, Leona D. Samson, Scott R. Floyd, Bevin P. Engelward,*
Link: https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8439179/
DNA damage can be cytotoxic and mutagenic, and it is directly linked to aging, cancer, and other diseases. To counteract the deleterious effects of DNA damage, cells have ...
Snapshots: No snapshots
Norah Owiti, Joshua Corrigan, Lee Pribyl, Jennifer Kay, Bevin Engelward
https://doi.org/10.3390/ijms231911776
The comet assay is a versatile assay for detecting DNA damage in eukaryotic cells. The assay can measure the levels of various types of damage, including DNA strand breaks, abasic sites and alkali-sensitive sites. Furthermore, the assay can also be modified to include purified DNA glycosylases so that alkylated and oxidized bases can be detected. The ...
Submitter: Charles Demurjian
Investigation: MIT SRP
Assays: Chemical Challenge - Metadata, Comet Chip - Data Linked, Comet Chip Analysis - Data Attached, Tissue Collection - Metadata
Snapshots: Snapshot 1
Irene Hu and Harold Hemond
Aquatic eddy covariance (AEC) is an in situ technique for measuring fluxes in marine and freshwater systems based on the covariance of velocity and concentration measurements. Here, development of a fast multiple-channel sensor (FACT) enables the use of AEC for measurement of benthic fluxes of fluorescent dissolved organic material, salt, and heat at three distinct sites in Massachusetts, USA, including the ...
Snapshots: Snapshot 1
Jennifer E. Kay, Joshua J. Corrigan, Amanda L. Armijo, Ilana S. Nazari, Ishwar N. Kohale, Dorothea K. Torous, Svetlana L. Avlasevich, Robert G. Croy, Dushan N. Wadduwage, Sebastian E. Carrasco, Stephen D. Dertinger, Forest M. White, John M. Essigmann, Leona D. Samson, Bevin P. Engelward
doi: https://doi.org/10.1101/2021.01.12.426356
Link to all of the Imaging done in this study: https://omero.mit.edu/webclient/?show=project-153 ...
Submitter: Dikshant Pradhan
Investigation: MIT SRP
Assays: GPT Assay – Data Attached, Mass Spectrometry Processing – Data Linked, Mass Spectrometry – Data Linked, Mouse Necropsy – Metadata, RaDR Image Machine Learning Analysis – Data Attached, Tissue Imaging – Metadata, Tissue Lysis – Metadata
Snapshots: Snapshot 1, Snapshot 2, Snapshot 3, Snapshot 4
Haosheng Feng, Shao-Xiong Lennon Luo, Robert G Croy, John M. Essigmann, Timothy M. Swager
https://doi.org/10.1039/D2DT03848J
Cu(I) from tetrakis(acetonitrile)copper(I) hexafluorophosphate ([Cu(MeCN)4]PF6) was complexed with five structurally related phosphines containing N-heterocycles. The interactions between the resulting complexes and some N-nitrosamines were studied using X-ray crystallography as well as emission spectroscopy. Upon complexation, three ...
Submitter: Charles Demurjian
Investigation: MIT SRP
Assays: Absorption and Emission Spectroscopy - Data Linked, Absorption and Emission Spectroscopy Analysis - Data Linked, Chemical Synthesis - Metadata, Crystallography - Data Linked, Electron Paramagnetic Resonance - Data Linked, High Resolution Mass Spectra - Data Linked, High Resolution Mass Spectra Analysis - Data Linked, Nuclear Magnetic Resonance Spectroscopy - Data Linked, Nuclear Magnetic Resonance Spectroscopy Analysis - Data Linked, X-ray Photoelectron Spectroscopy - Data Linked
Snapshots: Snapshot 1
Amanda L. Armijo, Pennapa Thongaram, Bogdan I. Fedeles, Judy Yau, Jennifer E Kay, Joshua J. Corrigan, Marisa Chancharoen, Supawadee Chawanthayatham, Leona D. Samson, Sebastian E. Carrasco, Bevin P. Engelward, James G. Fox, Robert G. Croy & John M. Essigmann
DNA-methylating environmental carcinogens such as N-nitrosodimethylamine (NDMA) and certain alkylators used in chemotherapy form O6-methylguanine (m6G) as a functionally critical intermediate. NDMA ...
Submitter: Charles Demurjian
Investigation: MIT SRP
Assays: Extraction and Library Creation - Metadata, GPT Assay - Data Attached, Genome Alignment - Data Linked, Illumina Sequencing - Data Linked, Mutational Spectral Analysis - Data Attached, Necropsy - Metadata
Snapshots: Snapshot 1
Submitter: Dikshant Pradhan
Assay type: Experimental Assay Type
Technology type: Technology Type
Investigation: MIT SRP
Organisms: No organisms
SOPs: P.ENG-200806-V2_Short-Term-Collection-at-Necrop..., P.ENG-201008-V1_Med-Term-Collection-at-Necropsy...
Data files: samples-publish2021-03-30_16-25.xlsx, samples-publish2021-03-30_17-38.xlsx, samples-publish2021-09-20_22-36.xlsx
Snapshots: No snapshots
Submitter: Dikshant Pradhan
Assay type: Experimental Assay Type
Technology type: Technology Type
Investigation: MIT SRP
Organisms: No organisms
SOPs: No SOPs
Data files: samples-publish2021-03-30_16-04.xlsx
Snapshots: No snapshots
Submitter: Dikshant Pradhan
Assay type: Experimental Assay Type
Technology type: Technology Type
Investigation: MIT SRP
Organisms: No organisms
SOPs: No SOPs
Data files: samples-publish2021-03-30_15-51.xlsx, samples-publish2021-03-30_17-07.xlsx
Snapshots: No snapshots
Submitter: Dikshant Pradhan
Assay type: Experimental Assay Type
Technology type: Technology Type
Investigation: MIT SRP
Organisms: No organisms
SOPs: No SOPs
Data files: samples-publish2021-03-30_14-31.xlsx, samples-publish2021-03-30_15-51.xlsx
Snapshots: No snapshots
Submitter: Dikshant Pradhan
Assay type: Experimental Assay Type
Technology type: Technology Type
Investigation: MIT SRP
Organisms: No organisms
SOPs: No SOPs
Data files: samples-publish2021-03-30_17-07.xlsx
Snapshots: No snapshots
Submitter: Dikshant Pradhan
Assay type: Experimental Assay Type
Technology type: Technology Type
Investigation: MIT SRP
Organisms: No organisms
SOPs: P.ESS-201123-V1_gpt-NextGen-Protocol.pdf
Data files: 10.1016.j.celrep.2021.108864_GPT_assay, D.GPT-samples-publish2021-12-13_23-22.xlsx
Snapshots: No snapshots
Submitter: Dikshant Pradhan
Assay type: Experimental Assay Type
Technology type: Technology Type
Investigation: MIT SRP
Organisms: No organisms
SOPs: No SOPs
Data files: A.IMG-samples-publish2021-12-13_22-22.xlsx, WAD_RaDR_roi_extraction_10.1016.j.celrep.2021.1..., samples-publish2021-03-30_16-04.xlsx
Snapshots: No snapshots
Submitter: Charles Demurjian
Assay type: Experimental Assay Type
Technology type: Technology Type
Investigation: MIT SRP
Organisms: No organisms
SOPs: P.ENG-230913-V1_Chemicals-and-Reagents.docx, P.ENG-230913-V1_In-Vivo-Comet-Chip---MUS.docx
Data files: CHM-samples-publish2023-09-13_11-34.xlsx, MUS-samples-publish2023-09-13_11-48.xlsx
Snapshots: No snapshots
Submitter: Charles Demurjian
Assay type: Experimental Assay Type
Technology type: Technology Type
Investigation: MIT SRP
Organisms: No organisms
SOPs: P.ENG-230912-V1_MMS-and-NDMA-TIS.docx, P.ENG-230913-V1_In-Vivo-Comet-Chip---MUS.docx
Data files: MUS-samples-publish2023-09-13_11-48.xlsx, TIS-samples-publish2023-09-13_12-27.xlsx
Snapshots: No snapshots
Submitter: Charles Demurjian
Assay type: Experimental Assay Type
Technology type: Technology Type
Investigation: MIT SRP
Organisms: No organisms
SOPs: P.ENG-230912-V1_Cell-Culture.docx, P.ENG-230912-V1_In-Vivo-Comet-Assay-Protocol.docx
Data files: CEL-samples-publish2023-09-19_13-11.xlsx, D.IMG-samples-publish2023-09-13_12-43.xlsx, TIS-samples-publish2023-09-13_12-27.xlsx
Snapshots: No snapshots
Submitter: Charles Demurjian
Assay type: Experimental Assay Type
Technology type: Technology Type
Investigation: MIT SRP
Organisms: No organisms
SOPs: P.ENG-230912-V1_In-Vivo-Comet-Assay-Protocol.docx
Data files: Figure 2B MMS 211203.pzfx, Figure 3A hAAG 211201_Enzymes.pzfx, Figure 3B MMS FPG.pzfx, Figure 4B.pzfx, Figure 5A Liver Time Course.pzfx, Figure 5B Lung time course.pzfx, Figure 5C Kidney NDMA Time Course.pzfx, Figure 6A Liver hAAG.pzfx, Figure 6B Liver Fpg.pzfx, Figure 6C-D Lung with Enzymes.pzfx, Figure 6E-F In_vivo_NDMA_Kidney w Enzymes copy...., Figure S2 MEFs and HepG2.pzfx, Figure S5A In_vivo_NDMA_Pancreas.pzfx, Figure S5B NDMA Spleen.pzfx
Snapshots: No snapshots
Submitter: Charles Demurjian
Assay type: Experimental Assay Type
Technology type: Technology Type
Investigation: MIT SRP
Organisms: No organisms
SOPs: P.SWA-240108-V2_Chemical_Synthesis-Agglutinatio...
Data files: samples-publish2024-02-10-CHM.xlsx
Snapshots: No snapshots
Submitter: Charles Demurjian
Assay type: Experimental Assay Type
Technology type: Technology Type
Investigation: MIT SRP
Organisms: No organisms
SOPs: P.SWA-240108-V2_Agglutination_Assay.docx
Data files: samples-publish2024-02-10-CHM.xlsx, samples-publish2024-02-10-D.IMG.xlsx
Snapshots: No snapshots
Submitter: Charles Demurjian
Assay type: Experimental Assay Type
Technology type: Technology Type
Investigation: MIT SRP
Organisms: No organisms
SOPs: P.SWA-240105-V1_NMR-Protocol.txt
Data files: samples-publish2024-02-10-CHM.xlsx, samples-publish2024-02-10-D.NMR.xlsx
Snapshots: No snapshots
Submitter: Charles Demurjian
Assay type: Experimental Assay Type
Technology type: Technology Type
Investigation: MIT SRP
Organisms: No organisms
SOPs: No SOPs
Data files: samples-publish2024-02-10-A.NMR.xlsx, samples-publish2024-02-10-D.NMR.xlsx
Snapshots: No snapshots
Submitter: Charles Demurjian
Assay type: Experimental Assay Type
Technology type: Technology Type
Investigation: MIT SRP
Organisms: No organisms
SOPs: P.SWA-240105-V1_GPC-Protocol.txt
Data files: samples-publish2024-02-10-CHM.xlsx, samples-publish2024-02-10-D.GPC.xlsx
Snapshots: No snapshots
Submitter: Charles Demurjian
Assay type: Experimental Assay Type
Technology type: Technology Type
Investigation: MIT SRP
Organisms: No organisms
SOPs: P.SWA-240105-V1_FTIR-Protocol.txt
Data files: samples-publish2024-02-10-CHM.xlsx, samples-publish2024-02-10-D.SPC.xlsx
Snapshots: No snapshots
Submitter: Charles Demurjian
Assay type: Experimental Assay Type
Technology type: Technology Type
Investigation: MIT SRP
Organisms: No organisms
SOPs: P.SWA-240105-V1_Pendant-Drop-Tensiometry-Protoc...
Data files: samples-publish2024-02-10-CHM.xlsx, samples-publish2024-02-10-D.TNSY.xlsx
Snapshots: No snapshots
Submitter: Charles Demurjian
Assay type: Experimental Assay Type
Technology type: Technology Type
Investigation: MIT SRP
Submitter: Charles Demurjian
Assay type: Experimental Assay Type
Technology type: Technology Type
Investigation: MIT SRP
Organisms: No organisms
SOPs: 2 hidden items
Data files: 14 hidden items
Snapshots: No snapshots
Batch sample publishing
Creator: Charles Demurjian
Submitter: Charles Demurjian
Investigations: MIT SRP
Batch sample publishing
Creator: Charles Demurjian
Submitter: Charles Demurjian
Investigations: MIT SRP
Studies: Novel In Vivo CometChip Reveals NDMA-Induced DN...
Assays: Comet Chip - Data Linked
Batch sample publishing
Creator: Charles Demurjian
Submitter: Charles Demurjian
Investigations: MIT SRP
Studies: Colorimetric Detection of Aqueous N-Nitrosodime...
Assays: UV-Vis Spectroscopy - Data Linked, UV-Vis Spectroscopy Analysis - Data Linked
Batch sample publishing
Creator: Charles Demurjian
Submitter: Charles Demurjian
Investigations: MIT SRP
Studies: Colorimetric Detection of Aqueous N-Nitrosodime...
Assays: Nuclear Magnetic Resonance - Data Linked, Nuclear Magnetic Resonance Analysis - Data Linked
Batch sample publishing
Creator: Charles Demurjian
Submitter: Charles Demurjian
Investigations: MIT SRP
Batch sample publishing
Creator: Charles Demurjian
Submitter: Charles Demurjian
Investigations: MIT SRP
Studies: Colorimetric Detection of Aqueous N-Nitrosodime...
Assays: Imaging - Data Linked
Batch sample publishing
Creator: Charles Demurjian
Submitter: Charles Demurjian
Investigations: MIT SRP
Studies: Colorimetric Detection of Aqueous N-Nitrosodime...
Assays: Imaging - Data Linked, Single-crystal X-ray Crystallography - Data Linked
Batch sample publishing
Creator: Charles Demurjian
Submitter: Charles Demurjian
Creator: Charles Demurjian
Submitter: Charles Demurjian
Investigations: MIT SRP
Studies: Optical Detection of Interleukin-6 using Liquid...
Assays: All Metadata
Batch sample publishing
Creator: Charles Demurjian
Submitter: Charles Demurjian
Investigations: MIT SRP
Batch sample publishing
Creator: Charles Demurjian
Submitter: Charles Demurjian
Investigations: MIT SRP
Batch sample publishing
Creator: Charles Demurjian
Submitter: Charles Demurjian
Investigations: MIT SRP
Batch sample publishing
Creator: Charles Demurjian
Submitter: Charles Demurjian
Investigations: MIT SRP
Batch sample publishing
Creator: Charles Demurjian
Submitter: Charles Demurjian
Batch sample publishing
Creator: Charles Demurjian
Submitter: Charles Demurjian
Investigations: MIT SRP
Batch sample publishing
Creator: Charles Demurjian
Submitter: Charles Demurjian
Investigations: MIT SRP
Studies: Optical Detection of Interleukin-6 using Liquid...
Assays: Agglutination Assay - Data Linked, Chemical Synthesis - Metadata, Fourier Transform Infrared Spectroscopy - Data ..., Gel Permeation Chromatography - Data Linked, Nuclear Magnetic Resonance Spectroscopy - Data ..., Pendant Drop Tensiometry - Data Linked
Batch sample publishing
Creator: Dikshant Pradhan
Submitter: Dikshant Pradhan
Investigations: MIT SRP
Studies: Excision of mutagenic replication-blocking lesi...
Assays: Mouse Necropsy – Metadata
Batch sample publishing
Creator: Dikshant Pradhan
Submitter: Dikshant Pradhan
Investigations: MIT SRP
Studies: Excision of mutagenic replication-blocking lesi...
Assays: Mass Spectrometry – Data Linked, Tissue Lysis – Metadata
Batch sample publishing
Creator: Dikshant Pradhan
Submitter: Dikshant Pradhan
Investigations: MIT SRP
Studies: Excision of mutagenic replication-blocking lesi...
Assays: Mouse Necropsy – Metadata
Batch sample publishing
Creator: Dikshant Pradhan
Submitter: Dikshant Pradhan
Investigations: MIT SRP
Studies: Excision of mutagenic replication-blocking lesi...
Assays: Mass Spectrometry Processing – Data Linked, Mass Spectrometry – Data Linked
General SOP of the overall study - contains info from both the manuscript and supplementary information packet.
Creator: Charles Demurjian
Submitter: Charles Demurjian
Investigations: MIT SRP
Studies: Colorimetric Detection of Aqueous N-Nitrosodime...
Assays: Chemical Synthesis - Metadata, Imaging - Data Linked, Nuclear Magnetic Resonance - Data Linked, Nuclear Magnetic Resonance Analysis - Data Linked, Single-crystal X-ray Crystallography - Data Linked, UV-Vis Spectroscopy - Data Linked, UV-Vis Spectroscopy Analysis - Data Linked
Creator: Charles Demurjian
Submitter: Charles Demurjian
Investigations: MIT SRP
Creator: Charles Demurjian
Submitter: Charles Demurjian
Investigations: MIT SRP
Creator: Charles Demurjian
Submitter: Charles Demurjian
Investigations: MIT SRP
Creator: Charles Demurjian
Submitter: Charles Demurjian
Investigations: MIT SRP
Creator: Charles Demurjian
Submitter: Charles Demurjian
Investigations: MIT SRP
Creator: Charles Demurjian
Submitter: Charles Demurjian
Investigations: MIT SRP
Studies: Optical Detection of Interleukin-6 using Liquid...
Assays: Chemical Synthesis - Metadata
Creator: Charles Demurjian
Submitter: Charles Demurjian
Investigations: MIT SRP
Studies: Novel In Vivo CometChip Reveals NDMA-Induced DN...
Assays: Chemical Challenge - Metadata, Tissue Collection - Metadata
Creator: Charles Demurjian
Submitter: Charles Demurjian
Investigations: MIT SRP
Studies: Novel In Vivo CometChip Reveals NDMA-Induced DN...
Assays: Tissue Collection - Metadata
Creator: Charles Demurjian
Submitter: Charles Demurjian
Investigations: MIT SRP
Studies: Novel In Vivo CometChip Reveals NDMA-Induced DN...
Assays: Comet Chip - Data Linked
Creator: Charles Demurjian
Submitter: Charles Demurjian
Investigations: MIT SRP
Studies: Novel In Vivo CometChip Reveals NDMA-Induced DN...
Assays: Comet Chip - Data Linked, Comet Chip Analysis - Data Attached
Creator: Charles Demurjian
Submitter: Charles Demurjian
Investigations: MIT SRP
Studies: Novel In Vivo CometChip Reveals NDMA-Induced DN...
Assays: Chemical Challenge - Metadata
Creator: Charles Demurjian
Submitter: Charles Demurjian
Investigations: MIT SRP
Creator: Charles Demurjian
Submitter: Charles Demurjian
Investigations: MIT SRP
Studies: Interaction of N-Nitroamines with Bincuelar Cop...
Assays: Chemical Synthesis - Metadata
Creator: Charles Demurjian
Submitter: Charles Demurjian
Creator: Charles Demurjian
Submitter: Charles Demurjian
Investigations: MIT SRP
Creator: Charles Demurjian
Submitter: Charles Demurjian
Investigations: MIT SRP
Creator: Charles Demurjian
Submitter: Charles Demurjian
Investigations: MIT SRP
Studies: Molecular origins of mutational spectra produce...
Assays: GPT Assay - Data Attached
Creator: Charles Demurjian
Submitter: Charles Demurjian
Investigations: MIT SRP
Studies: Molecular origins of mutational spectra produce...
Assays: Genome Alignment - Data Linked, Mutational Spectral Analysis - Data Attached
Creator: Charles Demurjian
Submitter: Charles Demurjian
Investigations: MIT SRP
Abstract
Authors: Jennifer E. Kay, Joshua J. Corrigan, Amanda L. Armijo, Ilana S. Nazari, Ishwar N. Kohale, Dorothea K. Torous, Svetlana L. Avlasevich, Robert G. Croy, Dushan N. Wadduwage, Sebastian E. Carrasco, Stephen D. Dertinger, Forest M. White, John M. Essigmann, Leona D. Samson, Bevin P. Engelward
Date Published: 1st Mar 2021
Publication Type: Journal
DOI: 10.1016/j.celrep.2021.108864
Citation: Cell Reports 34(11):108864