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Created: 13th Sep 2023 at 18:27
Last updated: 3rd Nov 2023 at 14:00
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Version 1 (earliest) Created 13th Sep 2023 at 18:27 by Charles Demurjian
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Projects: Hi-IMPAcTB, MIT SRP, MetNet, Endometriosis, Cancer Systems Biology Consortium (CSBC)
Institutions: Massachusetts Institute of Technology
Programme: MIT Superfund Research
Public web page: Not specified
Organisms: Not specified
Submitter: Dikshant Pradhan
Studies: Benthic fluxes of fluorescent dissolved organic material, salt and heat ..., Colorimetric Detection of Aqueous N-Nitrosodimethylamine via Photonitros..., CometChip Enables Parallel Analysis of Multiple DNA Repair Activities, Excision of mutagenic replication-blocking lesions suppresses cancer but..., Interaction of N-Nitroamines with Bincuelar Copper Complexs for Luminsec..., Molecular origins of mutational spectra produced by the environmental ca..., Novel In Vivo CometChip Reveals NDMA-Induced DNA Damage and Repair in Mu..., Optical Detection of Interleukin-6 using Liquid Janus Emulsions using Hy...
Assays: Absorption and Emission Spectroscopy - Data Linked, Absorption and Emission Spectroscopy Analysis - Data Linked, Agglutination Assay - Data Linked, All Metadata, Chemical Challenge - Metadata, Chemical Synthesis - Metadata, Chemical Synthesis - Metadata, Chemical Synthesis - Metadata, Comet Chip - Data Linked, Comet Chip Analysis - Data Attached, Comet Chip Analysis - Data Attached, Crystallography - Data Linked, Electron Paramagnetic Resonance - Data Linked, Extraction and Library Creation - Metadata, Field Water Sensor Run, Fourier Transform Infrared Spectroscopy - Data Linked, GPT Assay - Data Attached, GPT Assay – Data Attached, Gel Permeation Chromatography - Data Linked, Genome Alignment - Data Linked, High Resolution Mass Spectra - Data Linked, High Resolution Mass Spectra Analysis - Data Linked, Illumina Sequencing - Data Linked, Imaging - Data Linked, Mass Spectrometry Processing – Data Linked, Mass Spectrometry – Data Linked, Mouse Necropsy – Metadata, Mutational Spectral Analysis - Data Attached, Necropsy - Metadata, Nuclear Magnetic Resonance - Data Linked, Nuclear Magnetic Resonance Analysis - Data Linked, Nuclear Magnetic Resonance Spectroscopy - Data Linked, Nuclear Magnetic Resonance Spectroscopy - Data Linked, Nuclear Magnetic Resonance Spectroscopy Analysis - Data Linked, Nuclear Magnetic Resonance Spectroscopy Analysis - Data Linked, Pendant Drop Tensiometry - Data Linked, RaDR Image Machine Learning Analysis – Data Attached, Single-crystal X-ray Crystallography - Data Linked, Tissue Collection - Metadata, Tissue Imaging – Metadata, Tissue Lysis – Metadata, UV-Vis Spectroscopy - Data Linked, UV-Vis Spectroscopy Analysis - Data Linked, X-ray Photoelectron Spectroscopy - Data Linked
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Norah Owiti, Joshua Corrigan, Lee Pribyl, Jennifer Kay, Bevin Engelward
https://doi.org/10.3390/ijms231911776
The comet assay is a versatile assay for detecting DNA damage in eukaryotic cells. The assay can measure the levels of various types of damage, including DNA strand breaks, abasic sites and alkali-sensitive sites. Furthermore, the assay can also be modified to include purified DNA glycosylases so that alkylated and oxidized bases can be detected. The ...
Submitter: Charles Demurjian
Investigation: MIT SRP
Assays: Chemical Challenge - Metadata, Comet Chip - Data Linked, Comet Chip Analysis - Data Attached, Tissue Collection - Metadata
Snapshots: Snapshot 1
Submitter: Charles Demurjian
Assay type: Experimental Assay Type
Technology type: Technology Type
Investigation: MIT SRP
Organisms: No organisms
SOPs: P.ENG-230912-V1_In-Vivo-Comet-Assay-Protocol.docx
Data files: Figure 2B MMS 211203.pzfx, Figure 3A hAAG 211201_Enzymes.pzfx, Figure 3B MMS FPG.pzfx, Figure 4B.pzfx, Figure 5A Liver Time Course.pzfx, Figure 5B Lung time course.pzfx, Figure 5C Kidney NDMA Time Course.pzfx, Figure 6A Liver hAAG.pzfx, Figure 6B Liver Fpg.pzfx, Figure 6C-D Lung with Enzymes.pzfx, Figure 6E-F In_vivo_NDMA_Kidney w Enzymes copy...., Figure S2 MEFs and HepG2.pzfx, Figure S5A In_vivo_NDMA_Pancreas.pzfx, Figure S5B NDMA Spleen.pzfx
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