MIHCSME template example for “Temporal single cell cellular stress response activity toward toxicants inducing adaptive stress responses”
Compound screen on 13 HepG2 -GFP reporter lines, to measure GFP protein induction, and cell death induction. Template and associated files describe High Content Screening experimental data that conform to the MIHCSME specification.
SEEK ID: https://fairdomhub.org/assays/2027
Experimental assay
Projects: NL-Bioimaging FAIR Metadata Templates
Investigation: Minimal Information for High Content Screening in Microscopy Experiments (MIHCSME)
Study: MIHCSME templates
Assay position: 1
Assay type: high content screening
Technology type: Confocal Laser Scanning Microscopy (CLSM)
Organisms: No organisms
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Created: 25th Nov 2022 at 12:33
Last updated: 3rd Feb 2023 at 14:02
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https://orcid.org/0000-0002-3704-3675
NL-BioImaging AM is a distributed research infrastructure aimed at promoting progress in the most cutting edge microscopy technologies and providing open access to these technologies to a broad community of academic and industrial users. All Netherlands bio(medical) microscopy centers participate.
FAIR (Findable Accessible, Interoperable and Reusable) data management is a challenge for the optical microscopy community due to a high diversity in participating research fields and methodologies, ...
Projects: NL-Bioimaging FAIR Metadata Templates
Web page: http://eurobioimaging.nl/
This project collects and maintains minimum information metadata templates designed and used in the National NL-Bioimaging initiative. The templates are based on the REMBI standards and contribute to FAIR bioimaging data in the Netherlands, together with data management in the OMERO database.
Programme: NL-Bioimaging: FAIR-DM
Public web page: http://eurobioimaging.nl/
User metadata is an essential part of experimental data. Scientists need to understand underlying conditions and experimental procedures in order to model or investigate relevant biological questions. Currently, only a small fraction of the High Content SCreening (HCS) investigations are deposited for reuse by the community, and an even smaller fraction of that data is standards-compliant. For reusing data, scientists need to be able to understand how data was generated, under which experimental ...
Submitter: Katy Wolstencroft
Studies: MIHCSME templates
Assays: General MIHCSME template, MIHCSME template example for "Integration of biological data by kernels ..., MIHCSME template example for "Uncovering the signaling landscape control..., MIHCSME template example for compound screen on HepG2 CHOP-GFP reporter ..., MIHCSME template example for “Temporal single cell cellular stress respo...
A minimal metadata template for high content screening experiments in microscopy. The template is compliant with REMBI (Recommended Metadata for Biological Images) and the ISA framework (Investigations, Studies and Assays).
Here you will find guidelines for filling in MIHCSME metadata templates, as well as examples of published HCS studies that use MIHCSME.
Submitter: Rohola Hosseini
Investigation: Minimal Information for High Content Screening ...
Assays: General MIHCSME template, MIHCSME template example for "Integration of biological data by kernels ..., MIHCSME template example for "Uncovering the signaling landscape control..., MIHCSME template example for compound screen on HepG2 CHOP-GFP reporter ..., MIHCSME template example for “Temporal single cell cellular stress respo...
An example of MIHCSME describing live cell imaging HepG2 -GFP reporter line.
An example of MIHCSME describing live cell imaging HepG2 -GFP reporter line, describing assay with cell death staining.
This file is associated with performed study and describes protocols used.
Creator: Rohola Hosseini
Submitter: Rohola Hosseini
This document is associated with metadata file and describes compounds used in this study.
Creator: Rohola Hosseini
Submitter: Rohola Hosseini
