MIHCSME template example for "Uncovering the signaling landscape controlling breast cancer cell migration identifies novel metastasis driver genes"
MIHCSME template example for IDR0022 dataset. Primary and validation RNAi screen using smartpool and single siRNAs to identify cell migratory regulators in Hs578T and MDA-MB-231 triple-negative breast cancer cells.
SEEK ID: https://fairdomhub.org/assays/2040
Experimental assay
Projects: NL-Bioimaging FAIR Metadata Templates
Investigation: Minimal Information for High Content Screening in Microscopy Experiments (MIHCSME)
Study: MIHCSME templates
Assay position: 1
Assay type: high content screen of cells treated with library of siRNAs
Technology type: bright-field microscopy
Organisms: No organisms
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Created: 16th Jan 2023 at 08:58
Last updated: 3rd Feb 2023 at 14:02
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https://orcid.org/0000-0002-3704-3675
NL-BioImaging AM is a distributed research infrastructure aimed at promoting progress in the most cutting edge microscopy technologies and providing open access to these technologies to a broad community of academic and industrial users. All Netherlands bio(medical) microscopy centers participate.
FAIR (Findable Accessible, Interoperable and Reusable) data management is a challenge for the optical microscopy community due to a high diversity in participating research fields and methodologies, ...
Projects: NL-Bioimaging FAIR Metadata Templates
Web page: http://eurobioimaging.nl/
This project collects and maintains minimum information metadata templates designed and used in the National NL-Bioimaging initiative. The templates are based on the REMBI standards and contribute to FAIR bioimaging data in the Netherlands, together with data management in the OMERO database.
Programme: NL-Bioimaging: FAIR-DM
Public web page: http://eurobioimaging.nl/
User metadata is an essential part of experimental data. Scientists need to understand underlying conditions and experimental procedures in order to model or investigate relevant biological questions. Currently, only a small fraction of the High Content SCreening (HCS) investigations are deposited for reuse by the community, and an even smaller fraction of that data is standards-compliant. For reusing data, scientists need to be able to understand how data was generated, under which experimental ...
Submitter: Katy Wolstencroft
Studies: MIHCSME templates
Assays: General MIHCSME template, MIHCSME template example for "Integration of biological data by kernels ..., MIHCSME template example for "Uncovering the signaling landscape control..., MIHCSME template example for compound screen on HepG2 CHOP-GFP reporter ..., MIHCSME template example for “Temporal single cell cellular stress respo...
A minimal metadata template for high content screening experiments in microscopy. The template is compliant with REMBI (Recommended Metadata for Biological Images) and the ISA framework (Investigations, Studies and Assays).
Here you will find guidelines for filling in MIHCSME metadata templates, as well as examples of published HCS studies that use MIHCSME.
Submitter: Rohola Hosseini
Investigation: Minimal Information for High Content Screening ...
Assays: General MIHCSME template, MIHCSME template example for "Integration of biological data by kernels ..., MIHCSME template example for "Uncovering the signaling landscape control..., MIHCSME template example for compound screen on HepG2 CHOP-GFP reporter ..., MIHCSME template example for “Temporal single cell cellular stress respo...
An example template for screen published in IDR (idr0022, screenA).
An example template for screen published in IDR (idr0022, screenB).
