Binding of HK to Gre2p (ITC-BIND)

ITC binding (BIND) experiments to determine the binding parameters of HK ((5S,8S)-anti hydroxyketone) to Gre2p in 100 mM KPi Buffer. Experiments failed due to very weak binding and poor solubility of HK in buffer.


Experimental assay

Gudrun Gygli

Projects: Towards Reproducible Enzyme Modeling

Investigation: Workflow for characterization of enzymes under different reaction conditions

Study: ITC binding experiments

Assay position:

Assay type: Experimental Assay Type

Technology type: Technology Type

Organisms: No organisms

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Created: 21st Apr 2021 at 12:15

Last updated: 5th May 2021 at 12:02

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