Single nuclei RNA-Seq analysis of Fzt:DU mice
SEEK ID: https://fairdomhub.org/assays/1230
Modelling Analysis
Projects: iRhythmics
Investigation: hidden item
Study: Single nuclei characterisation of adult mammalian hearts
Assay position:
Biological problem addressed: Model Analysis Type
Organisms: No organisms
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Created: 24th Jan 2020 at 14:20
Last updated: 24th Jan 2020 at 16:35
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Projects: iRhythmics
Institutions: Leibniz Institute for Farm Animal Biology (FBN)
https://orcid.org/0000-0002-7968-3152
Projects that do not fall under current programmes.
Projects: Manchester Institute for Biotechnology, ICYSB 2015 - International Practical Course in Systems Biology, iRhythmics, INBioPharm, EmPowerPutida, Systo models, MycoSynVac - Engineering Mycoplasma pneumoniae as a broad-spectrum animal vaccine, Multiscale modelling of state transitions in the host-microbiome-brain network, Extremophiles metabolsim, NAD COMPARTMENTATION, Agro-ecological modelling, Bergen(Ziegler lab) project AF-NADase, NAMPT affinity, Stress granules, Modelling COVID-19 epidemics, Bio-crop, ORHIZON, Coastal Data, SASKit: Senescence-Associated Systems diagnostics Kit for cancer and stroke, hybrid sequencing, HOST-PAR, BioCreative VII, Boolean modeling of Parkinson disease map, Orphan cytochrome P450 20a1 CRISPR/Cas9 mutants and neurobehavioral phenotypes in zebrafish, Selective Destruction in Ageing, Viral Metagenomic, Synthetic biology in Synechococcus for bioeconomy applications (SynEco), testproject, SDBV ephemeral data exchanges, Test project, The BeeProject, PHENET, LiceVault, EbN1 Systems Biology
Web page: Not specified
The project addresses the generation and establishment of programmed pacemaker cells for an in vitro drug testing possibility to perform predictive tests. This may lead to an improved treatment of cardiac arrhythmias or an accurate identification of potential drug molecules at a very early stage of development. Important benefits will arise in verifying the safety of a wide variety of medicines while reducing animal testing. For more information you may visit our project website at ...
Programme: Independent Projects
Public web page: https://irhythmics.med.uni-rostock.de/
This study contains our single nuclei characterisation of whole hearts from Fzt.DU mice published in Cells.
Submitter: Markus Wolfien
Investigation: 1 hidden item
This dot-plot represents only the most significant genes per identified cell type cluster.
Creators: Markus Wolfien, Anne-Marie Galow
Submitter: Markus Wolfien
Relationship type: Not specified
This file contains the top markers for the identified cell types.
Creators: Markus Wolfien, Anne-Marie Galow
Submitter: Markus Wolfien
Relationship type: Not specified
This pdf contains the top 10 markers for each identified cell type as a dot-plot.
Creators: Markus Wolfien, Anne-Marie Galow
Submitter: Markus Wolfien
Relationship type: Not specified
This file contains the R-script to analyse single nuclei data previously processed with kallisto and bustools. The analyses utilizes the Seurat, harmony and RNAvelocity package.
Creators: Markus Wolfien, Anne-Marie Galow
Submitter: Markus Wolfien
Relationship type: Not specified
This file contains the detailed UNIX commands for mm10 index file creation (suitable for RNAvelocity) for kallisto as well as the commands used for the alignment with kallisto and the subsequent quantification with bustools.
This file contains the detailed experimental protocol to isolate nuclei from murine hearts.
Creators: Markus Wolfien, Anne-Marie Galow
Submitter: Markus Wolfien
