Single nuclei characterisation of adult mammalian hearts

This study contains our single nuclei characterisation of whole hearts from Fzt.DU mice published in Cells.

DOI: 10.15490/fairdomhub.1.study.713.1

Created at: 27th Jan 2020 at 14:16

Contents

Single nuclei RNA-Seq analysis of Fzt:DU mice

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UNIX script for snRNAseq

This file contains the detailed UNIX commands for mm10 index file creation (suitable for RNAvelocity) for kallisto as well as the commands used for the alignment with kallisto and the subsequent quantification with bustools.

  • UNIX script for snRNAseq Wolfien et al.txt

R script for snRNAseq analysis

This file contains the R-script to analyse single nuclei data previously processed with kallisto and bustools. The analyses utilizes the Seurat, harmony and RNAvelocity package.

  • R script for snRNAseq Wolfien et al.R

Top 10 marker genes per nuclei

This pdf contains the top 10 markers for each identified cell type as a dot-plot.

  • Top 10 marker genes per nuclei.pdf

Top 100 marker genes for whole heart fzt:DU cell types

This file contains the top markers for the identified cell types.

  • Marker_genes100_fztDU.xlsx

Top marker genes per nuclei

This dot-plot represents only the most significant genes per identified cell type cluster.

  • 1dot.pdf

Nuclei isolation for single nuclei RNA-seq

This file contains the detailed experimental protocol to isolate nuclei from murine hearts.

  • nuclei isolation_english.pdf
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Citation
Wolfien, M., Galow, A.-M., & Müller, P. (2020). Single nuclei characterisation of adult mammalian hearts. FAIRDOMHub. http://doi.org/10.15490/FAIRDOMHUB.1.STUDY.713.1
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Created: 27th Jan 2020 at 14:16

Last updated: 27th Jan 2020 at 14:18

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