MF_Flow_Proteomics_Protocol Version 1
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Created: 2nd Oct 2025 at 18:09
Last updated: 6th Oct 2025 at 19:00
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Version 1 (earliest) Created 2nd Oct 2025 at 18:10 by Charles Demurjian
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Projects: MIT SRP, Hi-IMPAcTB, MetNet, Endometriosis, Cancer Systems Biology Consortium (CSBC), Shoulders Lab, NExtSEEK Independent Projects
Web page: https://nextseek.mit.edu/
Understanding the spectrum of complex metastatic processes is important to the development of a comprehensive and cohesive understanding of cancer metastasis. The Metastasis Research Network (MetNet) supports collaborative, multidisciplinary projects that focus on several themes of the metastatic process and use integrative systems-level approaches.
MetNet is advancing the understanding of metastasis as a non-linear, dynamic, and emergent process, as well as revealing mechanistic insights of early ...
Programme: NExtSEEK
Public web page: Not specified
Submitter: Charles Demurjian
Studies: Chemo-mechanical diffusion waves explain collective dynamics of immune c..., Chromatin conformation, gene transcription, and nucleosome remodeling as..., Confined cell migration along extracellular matrix space in vivo, Impact of fibrinogen, fibrin thrombi and thrombin on cancer cell extrava..., IntravChip: a vascularized and perfused microfluidic model of the primar..., Myofibroblasts reduce angiogenesis and vasculogenesis in a vascularized ..., Patient-specific vascularized tumor model: Blocking monocyte recruitment..., Personalized Vascularized Models of Breast Cancer Desmoplasia Reveal Bio..., Polyploidy of MDA-MB-231 cells drives increased extravasation with enhan..., Remodeling of self-assembled microvascular networks under long term flow, Utilizing convolutional neural networks for discriminating cancer and st...
Assays: All Metadata, All Metadata, All Metadata, All Metadata, All Metadata, All Metadata, All Metadata, Cancer Cell Extravasation Analysis - Data Linked, Cancer Cell Extravasation Analysis - Data Linked, Cell Culture - Metadata, Cell Culture - Metadata, Cell Culture Imaging - Data Linked, Cell Culture Imaging Analysis - Data Linked, Cell Culture and Tumor Spheroid Creation - Metadata, Cell Culture and Tumor Spheroid Creation - Metadata, Cell Extraction - Metadata, Cell Imaging - Data Linked, Chromatin Sequencing Analysis - Data Linked, Clot Modeling Analysis - Data Linked, Convolutional Neural Network - Data Linked, Cytokine Luminex - Data Linked, Dendritic Cell Preparation - Metadata, Device Creation - Metadata, Device Creation - Metadata, Device Imaging - Data Linked, Device Imaging - Data Linked, Device Imaging - Data Linked, Device Imaging - Metadata, ELISA - Data Linked, Flow Cytometry - Data Linked, Flow Cytometry - Data Linked, Flow Cytometry Analysis - Data Linked, Flow Cytometry Analysis - Data Linked, Fluorescence-Activated Cell Sorting - Data Linked, Gene Expression Analysis - Data Linked, Gene Expression Analysis - Data Linked, Imaging - Data Linked, Imaging Analysis - Data Attached, Imaging Analysis - Data Linked, Imaging Analysis - Data Linked, Imaging Analysis - Data Linked, Imaging Analysis - Data Linked, Intravital Microscopy, Library Creation - Metadata, Library Prep - Metadata, Library Prep - Metadata, Live Cell Imaging - Data Linked, Luminex - Data Linked, MVN Imaging Analysis - Data Linked, Mass Spectrometry Proteomics - Data Linked, Microfluidic Device Creation - Metadata, Microfluidic Device Creation - Metadata, Microfluidic Device Imaging - Data Linked, Microfluidic Network Formation - Metadata, Microfluidic Network Formation - Metadata, Microfluidic Network Imaging - Data Linked, Microvascular Network Formation - Metadata, Migration Assay Analysis - Data Linked, Modeling - Data Linked, Modeling - Data Linked, Permeability Analysis - Data Linked, Proteomics Analysis - Data Linked, RNA Extraction - Metadata, RNA Extraction - Metadata, RNA Extraction - Metadata, Real Time RT-PCR - Data Linked, Real-time RT-PCR - Data Linked, Short Read Sequencing - Data Linked, Short Read Sequencing - Data Linked, Short Read Sequencing - Data Linked, Tumoroid Formation - Metadata
Marie Floryan, Elena Cambria, Adriana Blazeski, Mark F. Coughlin, Zhengpeng Wan, Giovanni Offeddu, Vinayak Vinayak, Aayush Kant, Vivek Shenoy, Roger D. Kamm
DOI:
Abstract: The incorporation of a functional perfusable microvascular network (MVN) is a common requirement for most organ on-chip-models. Long-term perfusion of MVNs is often required for the maturation of organ phenotypes and disease pathologies and to model the transport of cells and drugs entering ...
Submitter: Charles Demurjian
Investigation: MetNet
Assays: All Metadata, Cell Extraction - Metadata, Cytokine Luminex - Data Linked, Gene Expression Analysis - Data Linked, Library Prep - Metadata, MVN Imaging Analysis - Data Linked, Mass Spectrometry Proteomics - Data Linked, Microfluidic Network Formation - Metadata, Microfluidic Network Imaging - Data Linked, Proteomics Analysis - Data Linked, RNA Extraction - Metadata, Short Read Sequencing - Data Linked
Submitter: Charles Demurjian
Assay type: Experimental Assay Type
Technology type: Technology Type
Investigation: MetNet
Submitter: Charles Demurjian
Assay type: Experimental Assay Type
Technology type: Technology Type
Investigation: MetNet
