Assays
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030810H3 SD-LD
| Property | Value |
|---|---|
| BioDare ID | 13982027442235 |
| Author | Sarah Hodge |
| Institution | University of Edinburgh |
| License | CC_BY |
Description
Plants entrained in 8L:16D (120umol m-2s-1) for 7 days. Experimental Conditions: 2 days 8L:16D, 4 days 16L:8D (red and blue light 60umolm-2s-1)
Purpose
Re-entrainment dynamics during transition between light:dark cycles with different photoperiods, comparing Arabidopsis thaliana and ...
Submitter: Daniel Thedie
Assay type: Experimental Assay Type
Technology type: Technology Type
Investigation: Dixon Laura
Study: Dixon et al. (2014)
BW-detached_LL-LL_#20
| Property | Value |
|---|---|
| BioDare ID | 13394437504756 |
| Author | Benedicte Wenden |
| Institution | University of Edinburgh |
| License | CC_BY |
Description
Detached leaves of CCA1:LUC grown and imaged under constant light. Sample number 20 of Table S1 in the 2012 PNAS paper "Spontaneous spatiotemporal waves of gene expression from biological clocks in the leaf". The paper is attached as a file to sample #03.
Purpose
Testing ...
Submitter: Daniel Thedie
Assay type: Experimental Assay Type
Technology type: Technology Type
Investigation: Wenden Benedicte
Study: Wenden et al. (2012)
Edwards et al. (2010); WT in different light conditions.
| Property | Value |
|---|---|
| BioDare ID | 13227622661196 |
| Author | Edwards, K.D. |
| Institution | University of Edinburgh |
| License | CC_BY |
Description
Literature data from: 'Quantitative analysis of regulatory flexibility under changing environmental conditions.' by: Edwards, K.D..
Theory suggested that multiple loops contribute the flexibility for molecular rhythms to track multiple phases ...
Submitter: Daniel Thedie
Assay type: Experimental Assay Type
Technology type: Technology Type
Investigation: Kieron D Edwards
Study: Edwards et al. (2010)
Flis et al. TiMet clock gene comparison across photoperiods
| Property | Value |
|---|---|
| BioDare ID | 3967 |
| Author | Anna Flis |
| Institution | Max Planck Inst. Golm |
| License | CC_BY |
Description
Literature data from: 'Photoperiod-dependent changes in the phase of core clock transcripts and global transcriptional outputs at dawn and dusk in Arabidopsis' by: Anna Flis.
Col plants were grown in soil under 6, 8, 12 and 18h photoperiod for 21 days, and ...
Submitter: Daniel Thedie
Assay type: Experimental Assay Type
Technology type: Technology Type
Investigation: Flis Anna
Study: Flis et al. (2016)
Clock gene RNAs under RD-RR in clock mutants, expt 4
| Property | Value |
|---|---|
| BioDare ID | 13228357183121 |
| Author | Locke |
| Institution | |
| License |
Description
Literature data from: 'Extension of a genetic network model by iterative experimentation and mathematical analysis.' by: Locke.
First, heroic large-scale RNA profile screen by real-time qPCR in 96-well format, by Megan Southern in 2005. Experiment 4 of 4.
Purpose
Testing effects of clock ...
Submitter: Daniel Thedie
Assay type: Experimental Assay Type
Technology type: Technology Type
Investigation: Flis Anna
Study: Flis et al. (2015)
McWatters A.t. Col-0 WT clock RNA timeseries
| Property | Value |
|---|---|
| BioDare ID | 3488 |
| Author | Harriet G. McWatters |
| Institution | University of Oxford |
| License | CC_BY |
Description
Literature data from: 'Defining the robust behaviour of the plant clock gene circuit with absolute RNA timeseries and open infrastructure.' by: Harriet G. McWatters.
data are col0 8 d seedlings grown on sucrose in either LD 12:12 at 20 oC or WC 27:12 in LL. Gene ...
Submitter: Daniel Thedie
Assay type: Experimental Assay Type
Technology type: Technology Type
Investigation: Flis Anna
Study: Flis et al. (2015)
Q-PCR LD (12:12) 17C LHY
| Property | Value |
|---|---|
| BioDare ID | 3492 |
| Author | Aurora Pinas-Fernandez |
| Institution | University of Edinburgh |
| License | CC_BY |
Description
Literature data from: 'Temp' by: Aurora Pinas-Fernandez.
Wild-type and clock-mutant seedlings were entrained for 7 days at 22C in 12:12 white light. Then shifted to 17C 12:12 white light. Samples were collected on the 6th day at 17 degrees, every 2h, for 24h.
Purpose
...
Submitter: Daniel Thedie
Assay type: Experimental Assay Type
Technology type: Technology Type
Investigation: Flis Anna
Study: Flis et al. (2015)
Clock gene RNAs under RD-RR in clock mutants, expt 2
| Property | Value |
|---|---|
| BioDare ID | 13227619871305 |
| Author | Locke |
| Institution | |
| License |
Description
Literature data from: 'Extension of a genetic network model by iterative experimentation and mathematical analysis.' by: Locke.
First, heroic large-scale RNA profile screen by real-time qPCR in 96-well format, by Megan Southern in 2005. Experiment 2 of 4.
Purpose
Testing effects of clock ...
Submitter: Daniel Thedie
Assay type: Experimental Assay Type
Technology type: Technology Type
Investigation: Flis Anna
Study: Flis et al. (2015)
Clock gene RNAs under RD-RR in clock mutants, expt 3
| Property | Value |
|---|---|
| BioDare ID | 13228357055348 |
| Author | Locke |
| Institution | |
| License |
Description
Literature data from: 'Extension of a genetic network model by iterative experimentation and mathematical analysis.' by: Locke.
First, heroic large-scale RNA profile screen by real-time qPCR in 96-well format, by Megan Southern in 2005. Experiment 3 of 4.
Purpose
Testing effects of clock ...
Submitter: Daniel Thedie
Assay type: Experimental Assay Type
Technology type: Technology Type
Investigation: Flis Anna
Study: Flis et al. (2015)
Q-PCR LD (12:12) 17C ELF3
| Property | Value |
|---|---|
| BioDare ID | 12962294335805 |
| Author | Aurora Pinas-Fernandez |
| Institution | University of Edinburgh |
| License |
Description
Seedlings were entrained for 7 days at 22C in 12:12 white light. Then shifted to 17C 12:12 white light. Samples were collected on the 6th day at 17 degrees.
Purpose
Experiment carry out to reparameterize the clock off Succrose
Comments
Sample time 24h. 24h sample ...
Submitter: Daniel Thedie
Assay type: Experimental Assay Type
Technology type: Technology Type
Investigation: Flis Anna
Study: Flis et al. (2015)
Clock gene RNAs under RD-RR in clock mutants, expt 1
| Property | Value |
|---|---|
| BioDare ID | 13228298288040 |
| Author | Locke |
| Institution | University of Warwick |
| License |
Description
Literature data from: 'Extension of a genetic network model by iterative experimentation and mathematical analysis.' by: Locke.
First, heroic large-scale RNA profile screen by real-time qPCR in 96-well format, by Megan Southern in 2005. Data are noisy (especially ...
Submitter: Daniel Thedie
Assay type: Experimental Assay Type
Technology type: Technology Type
Investigation: Flis Anna
Study: Flis et al. (2015)
Q-PCR LD (12:12) 17C CAB2
| Property | Value |
|---|---|
| BioDare ID | 13228354371807 |
| Author | Aurora Pinas-Fernandez |
| Institution | University of Edinburgh |
| License | CC_BY |
Description
Seedlings were entrained for 7 days at 22C in 12:12 white light. Then shifted to 17C 12:12 white light. Samples were collected on the 6th day at 17 degrees.
Purpose
Experiment carry out to reparameterize the clock off Succrose
Comments
Sample time 24h. 24h ...
Submitter: Daniel Thedie
Assay type: Experimental Assay Type
Technology type: Technology Type
Investigation: Flis Anna
Study: Flis et al. (2015)
Q-PCR LD (12:12) 17C ELF4
| Property | Value |
|---|---|
| BioDare ID | 12962296599986 |
| Author | Aurora Pinas-Fernandez |
| Institution | University of Edinburgh |
| License | CC_BY |
Description
Seedlings were entrained for 7 days at 22C in 12:12 white light. Then shifted to 17C 12:12 white light. Samples were collected on the 6th day at 17 degrees.
Purpose
Experiment carry out to reparameterize the clock off Succrose
Comments
Sample time 24h. 24h ...
Submitter: Daniel Thedie
Assay type: Experimental Assay Type
Technology type: Technology Type
Investigation: Flis Anna
Study: Flis et al. (2015)
Q-PCR LD (12:12) 17C GI
| Property | Value |
|---|---|
| BioDare ID | 12820606741450 |
| Author | Aurora Pinas-Fernandez |
| Institution | University of Edinburgh |
| License | CC_BY |
Description
Seedlings were entrained for 7 days at 22C in 12:12 white light. Then shifted to 17C 12:12 white light. Samples were collected on the 6th day at 17 degrees.
Purpose
Experiment carry out to reparameterize the clock off Succrose
Comments
Sample time 24h. 24h ...
Submitter: Daniel Thedie
Assay type: Experimental Assay Type
Technology type: Technology Type
Investigation: Flis Anna
Study: Flis et al. (2015)
Q-PCR LD (12:12) 17C LUX
| Property | Value |
|---|---|
| BioDare ID | 12820610913763 |
| Author | Aurora Pinas-Fernandez |
| Institution | University of Edinburgh |
| License | CC_BY |
Description
Seedlings were entrained for 7 days at 22C in 12:12 white light. Then shifted to 17C 12:12 white light. Samples were collected on the 6th day at 17 degrees.
Purpose
Experiment carry out to reparameterize the clock off Succrose
Comments
Sample time 24h. 24h ...
Submitter: Daniel Thedie
Assay type: Experimental Assay Type
Technology type: Technology Type
Investigation: Flis Anna
Study: Flis et al. (2015)
Q-PCR LD (12:12) 17C TOC1
| Property | Value |
|---|---|
| BioDare ID | 12820611587928 |
| Author | Aurora Pinas-Fernandez |
| Institution | University of Edinburgh |
| License | CC_BY |
Description
Seedlings were entrained for 7 days at 22C in 12:12 white light. Then shifted to 17C 12:12 white light. Samples were collected on the 6th day at 17 degrees.
Purpose
Experiment carry out to reparameterize the clock off Succrose
Comments
Sample time 24h. 24h ...
Submitter: Daniel Thedie
Assay type: Experimental Assay Type
Technology type: Technology Type
Investigation: Flis Anna
Study: Flis et al. (2015)
Q-PCR LD (12:12) 17C PRR7
| Property | Value |
|---|---|
| BioDare ID | 12820611319996 |
| Author | Aurora Pinas-Fernandez |
| Institution | University of Edinburgh |
| License | CC_BY |
Description
Seedlings were entrained for 7 days at 22C in 12:12 white light. Then shifted to 17C 12:12 white light. Samples were collected on the 6th day at 17 degrees.
Purpose
Experiment carry out to reparameterize the clock off Succrose
Comments
Sample time 24h. 24h ...
Submitter: Daniel Thedie
Assay type: Experimental Assay Type
Technology type: Technology Type
Investigation: Flis Anna
Study: Flis et al. (2015)
Q-PCR LD (12:12) 17C PRR5
| Property | Value |
|---|---|
| BioDare ID | 12820611188065 |
| Author | Aurora Pinas-Fernandez |
| Institution | University of Edinburgh |
| License | CC_BY |
Description
Seedlings were entrained for 7 days at 22C in 12:12 white light. Then shifted to 17C 12:12 white light. Samples were collected on the 6th day at 17 degrees.
Purpose
Experiment carry out to reparameterize the clock off Succrose
Comments
Sample time 24h. 24h ...
Submitter: Daniel Thedie
Assay type: Experimental Assay Type
Technology type: Technology Type
Investigation: Flis Anna
Study: Flis et al. (2015)
Q-PCR LD (12:12) 17C PRR9
| Property | Value |
|---|---|
| BioDare ID | 12820610743262 |
| Author | Aurora Pinas-Fernandez |
| Institution | University of Edinburgh |
| License | CC_BY |
Description
Seedlings were entrained for 7 days at 22C in 12:12 white light. Then shifted to 17C 12:12 white light. Samples were collected on the 6th day at 17 degrees.
Purpose
Experiment carry out to reparameterize the clock off Succrose
Comments
Sample time 24h. 24h ...
Submitter: Daniel Thedie
Assay type: Experimental Assay Type
Technology type: Technology Type
Investigation: Flis Anna
Study: Flis et al. (2015)
TiMet WP1.1 WS seedling vs rosette
| Property | Value |
|---|---|
| BioDare ID | 2843 |
| Author | Anna Flis |
| Institution | MPI of Molecular Plant Physiology |
| License | CC_BY |
Description
Ws in biological duplicates, from diurnal cycle (12L/12D) with different plants age: 9 and 20 days. Numbers are in transcript copy per cell, obtained assuming 1 g FW contains 25000000 cells.
Purpose
Compare clock important genes transcript levels in seedlings ...
Submitter: Daniel Thedie
Assay type: Experimental Assay Type
Technology type: Technology Type
Investigation: Flis Anna
Study: Flis et al. (2015)
Q-PCR LD (12:12) 17C CCA1
| Property | Value |
|---|---|
| BioDare ID | 12820611467827 |
| Author | Aurora Pinas-Fernandez |
| Institution | University of Edinburgh |
| License | CC_BY |
Description
Seedlings were entrained for 7 days at 22C in 12:12 white light. Then shifted to 17C 12:12 white light. Samples were collected on the 6th day at 17 degrees.
Purpose
Experiment carry out to reparameterize the clock off Succrose
Comments
Sample time 24h. 24h ...
Submitter: Daniel Thedie
Assay type: Experimental Assay Type
Technology type: Technology Type
Investigation: Flis Anna
Study: Flis et al. (2015)
TiMet WP1.1 qRT-PCR LD to LL and LD
| Property | Value |
|---|---|
| BioDare ID | 2841 |
| Author | Anna Flis |
| Institution | MPI of Molecular Plant Physiology, Golm, Germany |
| License | CC_BY |
Description
2 WT (Col, Ws) and 5 clock mutants, in biological duplicates, from three conditions: Diurnal cycle (12L/12D), Extended night (DD), Extended light (LL), harvest every 2 hours. Numbers are in transcript copy per cell, obtained ...
Submitter: Daniel Thedie
Assay type: Experimental Assay Type
Technology type: Technology Type
Investigation: Flis Anna
Study: Flis et al. (2015)
TiMet WP1.1 qRT-PCR elf3 LD
| Property | Value |
|---|---|
| BioDare ID | 2842 |
| Author | Anna Flis |
| Institution | MPI of Molecular Plant Physiology |
| License | CC_BY |
Description
WT and elf3-4 mutants, in biological duplicates, diurnal light, harvest every 2 hours Numbers are in transcript copy per cell, obtained assuming 1 g FW contains 25000000 cells.
Purpose
TiMet focuses on an important and general biological problem – how are plant ...
Submitter: Daniel Thedie
Assay type: Experimental Assay Type
Technology type: Technology Type
Investigation: Flis Anna
Study: Flis et al. (2015)
Experimental data for the initial rate kinetics of EDA for KDPG, OAA, and KHG.
Submitter: Jacky Snoep
Assay type: Experimental Assay Type
Technology type: Technology Type
Investigation: The cyanobacterium Synechocystis has a promiscu...
Study: Kinetic characterisation
Mathematica notebook for MM model fit to the initial rate kinetics of EDA
Submitter: Jacky Snoep
Biological problem addressed: Model Analysis Type
Investigation: The cyanobacterium Synechocystis has a promiscu...
Study: Kinetic characterisation
Submitter: Charles Demurjian
Assay type: Experimental Assay Type
Technology type: Technology Type
Investigation: IMPAcTB
Submitter: Charles Demurjian
Assay type: Experimental Assay Type
Technology type: Technology Type
Investigation: IMPAcTB
Submitter: Charles Demurjian
Assay type: Experimental Assay Type
Technology type: Technology Type
Investigation: IMPAcTB
Submitter: Charles Demurjian
Assay type: Experimental Assay Type
Technology type: Technology Type
Investigation: IMPAcTB
Submitter: Charles Demurjian
Assay type: Experimental Assay Type
Technology type: Technology Type
Investigation: IMPAcTB