Assays

Short Name: Pc1599-EcoliExpr Assay Class: WET Assay Type: EcoliExpr Title: Candidate sequence from P. citri Pc1599 Description: Expression in E. coli and activity testing of Pc1599 pISA Assay creation date: 2019-07-01 pISA Assay creator: IG Candidate sequence gbk file (file path or link): _S_P4_expressionEcoli/reports/sequences/pMAL-c5XPc1599.gb Expression plasmid: pMAL-c5X Host E coli strain: BL21(DE3)pLysS Expression temperature: 30C Date of sample collection: 2019-07-02 Sample purification ...

Short Name: Pc23180-EcoliExpr Assay Class: WET Assay Type: EcoliExpr Title: Candidate sequence from P. citri Pc23180 Description: Expression in E. coli and activity testing of Pc23180 pISA Assay creation date: 2019-09-03 pISA Assay creator: IG Candidate sequence gbk file (file path or link): _S_P4_expressionEcoli/reports/sequences/pMAL-c5XPc23180.gb Expression plasmid: pMAL-c5X Host E coli strain: BL21(DE3)pLysS Expression temperature: 30C Date of sample collection: 2019-09-04 Sample purification ...

Files connected to investigation: _I_T31_mealybug

Files connected to study: _S_P3_expressionEcoli

Short Name: C98F-EcoliExpr Assay Class: WET Assay Type: EcoliExpr Title: C98F chimeric protein Description: Expression in E. coli and activity testing of C98F chimeric protein pISA Assay creation date: 2019-05-07 pISA Assay creator: IG Candidate sequence gbk file (file path or link): _S_P4_expressionEcoli/reports/sequences/C98F.gb Expression plasmid: pMAL-c5X Host E coli strain: BL21(DE3)pLysS Expression temperature: 30C Date of sample collection: 2019-05-08 Sample purification protocol (file ...

The soluble protein fraction was subjected to shotgun proteomics (i..e. in-solution digest) applying nanoLC ESI-Iontrap MS/MS and only detections in at least two replicates per condition and bacterium were considered for subsequent analyses. Correspondingly peptide count data was compiled per condition and strain studied.

The experimental data for PFK-1 and PFK-2 were analysed and fitted with a MWC equation. The model is described in Mathematica and the plots in the manuscript are generatedin the notebook.

The purified enzymes were kinetically charactyerised in terms of substrate sensitivity and alloestric regulation.

Training data for this Model is available: https://fairdomhub.org/studies/1198

A stock solution of BSA (Bovine serum Albumine) 1 mg/ml is made and from this solution different aliquots are picked in order to obtain different dilution solution.

1. 25 µL from solution + 975 µL water to obtain 25 µg/ml
2. 50 µL from solution + 950 µL of water to obtain 50 µg/ml
3. 75 µL from solution + 925 µL water to obtain 75 µg/ml
4. 100 µL from solution + 900 µL water to obtain 100 µg/ml
5. 125 µL from solution + 825 µL water to obtain 125 µg/ml
6. 150 µL from solution + 850 µL water to ...

A file combining all CpG sites from the differentially methylated regions (DMRs) of the control, t0, and t1 groups was generated. This combined file, containing methylation values extracted from the bedgraph files, was then used to create a PCA plot and a single heatmap for all CpG sites across the regions.