Assays
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Submitter: Markus Wolfien
Biological problem addressed: Gene Expression
Investigation: Disparate immune responses lead to varied outco...
Study: Single nuclei data analysis
Application of the LoRAS oversampling approach on single-cell/single-nuclei data to annotate/identify specific cell populations in new data based on previously, manually curated data.
Submitter: Markus Wolfien
Biological problem addressed: Annotation
Investigation: 1 hidden item
Study: 1 hidden item
Here, we conduct a proof of principle by comparing a 2D and 3D fluorescent image analysis based approach on unlabeled cardiomyocytes. Based on the CellProfiler software, we extracted high-dimensional features of individual cells and nuclei, which are subsequently down-sampled and clustered. These clusters are furthermore benchmarked via different machine learning classifiers (e.g., AdaBoost, Gradient Boosting, Random Forest) as the ground truth for our proposed approach.
Submitter: Markus Wolfien
Biological problem addressed: Model Analysis Type
Investigation: 1 hidden item
Study: 1 hidden item
Submitter: Markus Wolfien
Biological problem addressed: Model Analysis Type
Investigation: 1 hidden item
Submitter: Markus Wolfien
Biological problem addressed: Gene Expression
Investigation: 1 hidden item
Study: Single nuclei comparison