Single nuclei RNA-Seq analysis of Fzt:DU and BL6 mice
DownloadDOI: 10.15490/fairdomhub.1.assay.1227.1
Zenodo URL: None
Created at: 27th Feb 2020 at 11:09
Nuclei characteristics for the single nuclei analysis of Fzt:DU and BL6 mice
File contains the detailed cluster names for each data set, number of nuclei per cluster, average reads per nucleus, and average reads per cluster.
- Nuclei.xlsx
Marker genes for single nuclei clusters
This file contains the IDs, adj. p-values and official gene names of the top 100 marker genes (where applicable) for each of the identified cluster.
- Marker_genes100_integrated.csv
UNIX script for snRNA-Seq processing
No description specified
- UNIX script for snRNAseq Wolfien et al.txt
R script for single nuclei analysis
This R-script contains the single nuclei analysis for our Fzt:DU and BL6 data, namely bustools file integration, preprocessing, scaling, clustering, marker indentification and annotation of markers and RNAvelocity computation.
- R script for snRNAseq Wolfien et al.R
Dotplot for the top 100 markers per cluster
This PDF contains a dotplot indicating the gene expression of the top 100 markers (where applicable) for each of the identified 23 clusters.
- top100markers_per_cluster.pdf
Materials and Methods
This file contains a detailed description of the underlying experimental procedure and the computational analysis.
- Materials and Methods snRNA-seq.pdf
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Created: 27th Feb 2020 at 11:09
Last updated: 27th Feb 2020 at 11:09
