Single nuclei RNA-Seq analysis of Fzt:DU and BL6 mice

No description specified

DOI: 10.15490/fairdomhub.1.assay.1227.1

Zenodo URL: None

Created at: 27th Feb 2020 at 12:09

Contents

Nuclei characteristics for the single nuclei analysis of Fzt:DU and BL6 mice

File contains the detailed cluster names for each data set, number of nuclei per cluster, average reads per nucleus, and average reads per cluster.

  • Nuclei.xlsx

Marker genes for single nuclei clusters

This file contains the IDs, adj. p-values and official gene names of the top 100 marker genes (where applicable) for each of the identified cluster.

  • Marker_genes100_integrated.csv

UNIX script for snRNA-Seq processing

No description specified

  • UNIX script for snRNAseq Wolfien et al.txt

R script for single nuclei analysis

This R-script contains the single nuclei analysis for our Fzt:DU and BL6 data, namely bustools file integration, preprocessing, scaling, clustering, marker indentification and annotation of markers and RNAvelocity computation.

  • R script for snRNAseq Wolfien et al.R

Dotplot for the top 100 markers per cluster

This PDF contains a dotplot indicating the gene expression of the top 100 markers (where applicable) for each of the identified 23 clusters.

  • top100markers_per_cluster.pdf

Materials and Methods

This file contains a detailed description of the underlying experimental procedure and the computational analysis.

  • Materials and Methods snRNA-seq.pdf
Fingerprints

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MD5: dd4c9ccba9f0a06e350ab66cb6a6789f

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Citation
Wolfien, M., Galow, A.-M., & Müller, P. (2020). Single nuclei RNA-Seq analysis of Fzt:DU and BL6 mice. FAIRDOMHub. https://doi.org/10.15490/FAIRDOMHUB.1.ASSAY.1227.1
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Snapshot 1 (27th Feb 2020) DOI
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Created: 27th Feb 2020 at 12:09

Last updated: 27th Feb 2020 at 12:09

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