Assays

Files connected to investigation: _I_T32_CandidateGeneExpressionTesting

Files connected to study: _S_TransientLPPSCPPS

Short Name: TransientCompLPPSCPPS-GCMS Assay Class: WET Assay Type: GCMS Title: Transient expression of LPPS and CPPS genes in Nicotiana benthamiana plants Description: The purpose of this assay is to check the transient expression of the LPPS and CPPS genes in Nicotiana benthamiana plants. Two different Agrobacterium strains were assayed and compared the levels of production between them. Volatile monoterpenoid levels were measured by GCMS of samples. pISA Assay creation date: 2021-12-03 pISA ...

Files connected to study: _S_P5_PhEmissionPlants

Short Name: emission_nontruncated-GC-MS Assay Class: WET Assay Type: GC-MS Title: Pheromone emission of non-truncated SxP plants Description: Quantification of the pheromone emitted by the non-truncated SxP versions that were producing both Z11C16OL and Z11C16AC pISA Assay creation date: 21-Nov-16 pISA Assay creator: Sandra Vacas Phenodata: None Featuredata: Data:

Short Name: emission_truncated-GC-MS Assay Class: WET Assay Type: GC-MS Title: Pheromone emitted by truncated versions of SxP Description: Quantification of the pheromone emitted by truncated versions of SxP plants that were not producing Z11C16AC pISA Assay creation date: 21-Nov-16 pISA Assay creator: Sandra Vacas Phenodata: None Featuredata: Data:

Files connected to study: _S_P5_PhContentPlants

Short Name: GC-MS-EAD-EAG Assay Class: WET Assay Type: EAG Title: EAG recordings coupled to GC-MS Description: EAG recordings of stimuli provided to Sesamia nonagrioides male antenna by the effluent of the GC-MS pISA Assay creation date: 21-Nov-16 pISA Assay creator: Sandra Vacas Phenodata: None Featuredata: Data:

Short Name: PhCont_fr20-GC-MS Assay Class: WET Assay Type: GC-MS Title: Quantification of pheromone content in leaves frozen at -20 Description: Quantification of the pheromone content in SxP v1.2 plant leaves frozen at -20C that produce both Z11C16OL nad Z11C16AC pISA Assay creation date: 21-Nov-17 pISA Assay creator: Sandra Vacas Phenodata: None Featuredata: Data:

Short Name: PhCont_fr80-GC-MS Assay Class: WET Assay Type: GC-MS Title: Quantification of pheromone content in leaves frozen at -80 Description: Quantifiction of pheromone content in SxP v1.2 plant leaves frozen at -80C that produce both Z11C16OL and Z11C16AC pISA Assay creation date: 21-Nov-17 pISA Assay creator: Sandra Vacas Phenodata: None Featuredata: Data:

Short Name: PhCont_fresh-GC-MS Assay Class: WET Assay Type: GC-MS Title: Pheromone content in fresh leaves Description: Quantification of the pheromone content in fresh leaves of SxP v1.2 plants that produce both Z11C16OL and Z11C16AC pISA Assay creation date: 21-Nov-17 pISA Assay creator: Sandra Vacas Phenodata: None Featuredata: Data:

Files connected to investigation: _I_T24_phero

Files connected to study: _S_P5_EAGresponse

Short Name: EAG_manual-EAG Assay Class: WET Assay Type: EAG Title: EAG recordings of manual stimuli Description: EAG recordings of stimulus manually provided to Sesamia nonagrioides male antenna pISA Assay creation date: 21-Nov-16 pISA Assay creator: Sandra Vacas Phenodata: None Featuredata: Data:

Files connected to study: _S_P5_SxFliquids

Files connected to study: _S_P5_SxFmycelia

Short Name: FS-GC-MS Assay Class: WET Assay Type: GC-MS Title: Full Scan analysis of SxF liquid samples Description: Full Scan analysis of the solvent extracts of SxF liquid samples pISA Assay creation date: 21-Sep-27 pISA Assay creator: Sandra Vacas Phenodata: None Featuredata: Data:

Short Name: SRM-GC-MS Assay Class: WET Assay Type: GC-MS Title: SRM analysis of SxF liquid samples Description: GC-MS analysis in SRM mode of the solvent extracts of SxF liquid samples pISA Assay creation date: 21-Sep-27 pISA Assay creator: Sandra Vacas Phenodata: None Featuredata: Data:

Short Name: FSM-GC-MS Assay Class: WET Assay Type: GC-MS Title: Full Scan analysis of SxF mycelium extracts Description: GC-MS analysis in full scan mode of SxF mycelium solvent extracts pISA Assay creation date: 21-Sep-27 pISA Assay creator: Sandra Vacas Phenodata: None Featuredata: Data:

Short Name: SRMM-GC-MS Assay Class: WET Assay Type: GC-MS Title: SRM analysis on SxF mycelium extracts Description: GC-MS analysis in SRM mode of SxF mycelium extracts pISA Assay creation date: 21-Sep-27 pISA Assay creator: Sandra Vacas Phenodata: None Featuredata: Data:

Files connected to investigation: _I_T23_SxF

Files connected to study: _S_P1_ConstExp_Pdigitatum

Short Name: qRTPCRMothPheromonesEnzymes-Q-RT-PCR Assay Class: WET Assay Type: Q-RT-PCR Title: qRT-PCR Analysis of enzymes AtrD11 and HarFar in Penicillium digitatum transformants Description: Two Penicillium digitatum transformants were selected that showed integration of the two genes AtrD11 and HarFar (PCR test) but not moth pheromone production (GC-MS, analysed in CEQA facilities). These two transformants were then assayed in their expression of the transgenes by qRT-PCR. pISA Assay creation ...

Files connected to study: _S_P1_TransgenicStableSxPv2

Short Name: SxPdCasEVNonGuidedPathway-GCMS Assay Class: WET Assay Type: GCMS Title: SxP dCasEV Non Guided Pathway (EV NGP) Description: Generation and analysis of the stable Nicotiana benthamiana transgenic plants with the moth sex pheromone biosynthetic pathway genes regulated by synthetic promotors, the dCasEV system and not provided with the specific guide in stable. The activation of the pathway was performed by agroinfiltration of the specific guide in trans, so the system is complete and ...

Short Name: SxPGuidedPathway-GCMS Assay Class: WET Assay Type: GCMS Title: SxP Guided Pathway (GP) Description: Generation and analysis of the stable Nicotiana benthamiana transgenic plants with the moth sex pheromone biosynthetic pathway genes regulated by synthetic promotors and provided with the specific guide in stable. The purpose of this assay is to measure the volatile moth sex pheromone production by HSPME GCMS of the generated stable Nicotiana benthamiana plants. The activation of the ...

Short Name: SxPNonGuidedPathway-GCMS Assay Class: WET Assay Type: GCMS Title: SxP Non Guided Pathway (NGP) Description: Generation and analysis of the stable Nicotiana benthamiana transgenic plants with the moth sex pheromone biosynthetic pathway genes regulated by synthetic promotors and not provided with the specific guide in stable. The activation of the pathway was performed by agroinfiltration of the dCasEV and the specific guide in trans, so the system is complete and the production of the ...

Files connected to investigation: _I_T22_SxPv2

Files connected to study: _S_P1_TransientExp_Nb

Short Name: AgroinfFinalConstructsdCas9EV-GCMS Assay Class: WET Assay Type: GCMS Title: Agroinfiltration of final constructs for copper-inducible pathway in Nicotiana benthamiana leaves Description: Two constructs were made containing the three genes of the moth pheromone pathway under the regulation of synthetic promoters made in T11, nptII selection marker and with/without the gRNA1 for activation. In parallel, another construct containing the copper-inducible dCas9EV system with/without the ...