Short Name: T32_CandidateGeneExpressionTesting Title: Candidate gene expression and testing Description: The purpose of this investigation is to express transiently candidate genes for Pcitri sex pheromone biosynthesis. This genes could act either as genes catalyzing steps related to the biosynthesis of precursors of the pheromone itself, either for the final steps of the biosynthesis. After expression, the monoterpenoid production function of those genes is checked by GCMS. Phenodata: ./phenodata_20211203.txt Principal investigator: Diego Orzaez License: Creative Commons Attribution 4.0 Sharing permission: Private Upload to FAIRDOMHub: Yes Institutions involved: Instituto de Biología Molecular y Celular de Plantas (IBMCP), Spain; Institute of Agrochemistry and Food Technology (IATA), Spain; The Earlham Institute (EI), UK; National institute of Biology (NIB), Slovenia; Technische Universität Darmstadt (TUDA), Germany; Ecologia y Proteción Agrícola (EPA), Spain Project start date: 1.4.2018 Project end date: 31.3.2021
Investigation position:
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Created: 17th May 2022 at 14:15
Last updated: 7th Nov 2024 at 10:05
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PhD in Biotechnology, Research Associate at Department of Biotechnology and Systems Biology, National Institute of Biology
National Institute of Biology, Department of Biotechnology and Systems Biology projects
Projects: HYp - Spatiotemporal analysis of hypersensitive response to Potato virus Y in potato, pISA-tree, MOA - Multiomics analysis of potato response to Potato virus Y (PVY) infection, SUSPHIRE - Sustainable Bioproduction of Pheromones for Insect Pest Control in Agriculture, INDIE - Biotechnological production of sustainable indole, _p_stRT, ADAPT - Accelerated Development of multiple-stress tolerAnt PoTato, _p_RNAinVAL, tst, tst2, Playground
Web page: http://www.nib.si/eng/index.php/departments/department-of-biotechnology-and-systems-biology
SUSPHIRE aims to provide a sustainable, low-cost biomanufacturing platform for the commercial production of insect pheromones.
Programme: NIBSys
Public web page: http://susphire.info/susphireproject/
Start date: 1st Apr 2018
End date: 30th Sep 2021
Investigation files _I_T32_CandidateGeneExpressionTesting
Submitter: Marko Petek
Investigation: _I_T32_CandidateGeneExpressionTesting
Snapshots: No snapshots
Short Name: TransientLPPSCPPS Title: Transient expression of lavandulyl pirophosphate synthase (LPPS) and chrysanthemyl pirophhosphate synthase (CPPS) Description: The purpose of this study is to perform the transient expression of LPPS and CPPS gene for the production of lavandulol and chrysanthemol, respectively. Production is measured by GCMS of samples. Raw Data: pISA Study creation date: 2021-12-03 pISA Study creator: RMF Principal investigator: Diego Orzaez License: Creative Commons Attribution ...
Submitter: Marko Petek
Investigation: _I_T32_CandidateGeneExpressionTesting
Assays: _A_TransientCompLPPSCPPS-GCMS, _S_TransientLPPSCPPS-files
Snapshots: No snapshots
Files connected to investigation: _I_T32_CandidateGeneExpressionTesting
Submitter: Marko Petek
Assay type: Experimental Assay Type
Technology type: Technology Type
Investigation: _I_T32_CandidateGeneExpressionTesting
Study: Investigation files
Organisms: No organisms
SOPs: No SOPs
Data files: _INVESTIGATION_METADATA.TXT, phenodata_20200720.txt
Snapshots: No snapshots
Files connected to study: _S_TransientLPPSCPPS
Submitter: Marko Petek
Assay type: Experimental Assay Type
Technology type: Technology Type
Investigation: _I_T32_CandidateGeneExpressionTesting
Study: _S_TransientLPPSCPPS
Short Name: TransientCompLPPSCPPS-GCMS Assay Class: WET Assay Type: GCMS Title: Transient expression of LPPS and CPPS genes in Nicotiana benthamiana plants Description: The purpose of this assay is to check the transient expression of the LPPS and CPPS genes in Nicotiana benthamiana plants. Two different Agrobacterium strains were assayed and compared the levels of production between them. Volatile monoterpenoid levels were measured by GCMS of samples. pISA Assay creation date: 2021-12-03 pISA ...
Submitter: Marko Petek
Assay type: Experimental Assay Type
Technology type: Technology Type
Investigation: _I_T32_CandidateGeneExpressionTesting
Study: _S_TransientLPPSCPPS
_p_SUSPHIRE/_I_T32_CandidateGeneExpressionTesting/_S_TransientLPPSCPPS/
Creator: Marko Petek
Submitter: Marko Petek
Investigations: _I_T32_CandidateGeneExpressionTesting
Studies: _S_TransientLPPSCPPS
Assays: _S_TransientLPPSCPPS-files
_p_SUSPHIRE/_I_T32_CandidateGeneExpressionTesting/_S_TransientLPPSCPPS/_A_TransientCompLPPSCPPS-GCMS/
Creator: Marko Petek
Submitter: Marko Petek
Investigations: _I_T32_CandidateGeneExpressionTesting
Studies: _S_TransientLPPSCPPS
Assays: _A_TransientCompLPPSCPPS-GCMS
_p_SUSPHIRE/_I_T32_CandidateGeneExpressionTesting/
Creator: Marko Petek
Submitter: Marko Petek
Investigations: _I_T32_CandidateGeneExpressionTesting
Studies: Investigation files
_p_SUSPHIRE/_I_T32_CandidateGeneExpressionTesting/
Creator: Marko Petek
Submitter: Marko Petek
Investigations: _I_T32_CandidateGeneExpressionTesting
Studies: Investigation files
_p_SUSPHIRE/_I_T32_CandidateGeneExpressionTesting/_S_TransientLPPSCPPS/
Creator: Marko Petek
Submitter: Marko Petek
Investigations: _I_T32_CandidateGeneExpressionTesting
Studies: _S_TransientLPPSCPPS
Assays: _S_TransientLPPSCPPS-files
_p_SUSPHIRE/_I_T32_CandidateGeneExpressionTesting/_S_TransientLPPSCPPS/_A_TransientCompLPPSCPPS-GCMS/
Creator: Marko Petek
Submitter: Marko Petek
Investigations: _I_T32_CandidateGeneExpressionTesting
Studies: _S_TransientLPPSCPPS
Assays: _A_TransientCompLPPSCPPS-GCMS
_p_SUSPHIRE/_I_T32_CandidateGeneExpressionTesting/_S_TransientLPPSCPPS/_A_TransientCompLPPSCPPS-GCMS/
Creator: Marko Petek
Submitter: Marko Petek
Investigations: _I_T32_CandidateGeneExpressionTesting
Studies: _S_TransientLPPSCPPS
Assays: _A_TransientCompLPPSCPPS-GCMS