project: _p_stRT Short Name: stRT Title: Solanum tuberosum Reference Transcriptomes Description: Cultivar-specific transcriptome and pan-transcriptome reconstruction of tetraploid potato pISA projects path: ../pISA-Projects Local pISA-tree organisation: National Institute of Biology pISA project creation date: 2019-10-22 pISA project creator: Maja Zagorscak, Ziva Ramsak, Marko Petek Project funding code: This project was supported by the Slovenian Research Agency (grants P4-0165, J4-4165, J4-7636, J4-8228 and J4-9302), COST actions CA15110 (CHARME) and CA15109 (COSTNET). Project coordinator: Kristina Gruden Project partners: PacBio, Menlo Park, CA, USA; National Center for Genome Analysis and Support (NCGAS), Indiana University, Bloomington, USA; Laboratoire Génomique et Biotechnologie des Fruits, INRA-INP/ENSAT, Castanet-Tolosan, France Project start date: December 2017 Project end date: October 2019 Principal investigator: Kristina Gruden License: MIT, CC BY 4.0 Sharing permission: Public Upload to FAIRDOMHub: Yes
RELATED FILES: pISA/README.MD at projects · NIB-SI/pISA · GitHub https://fairdomhub.org/documents/350 /_PROJECT_METADATA.TXT https://fairdomhub.org/documents/329 /_p_stRT_TREE.TXT https://fairdomhub.org/documents/328 /presentations/README.MD https://fairdomhub.org/documents/335 /README.MD https://fairdomhub.org/documents/330 /reports/README.MD https://fairdomhub.org/documents/332 /Metadata_files https://fairdomhub.org/documents/331
Programme: NIBSys
SEEK ID: https://fairdomhub.org/projects/161
Funding codes:- P4-0165
- J4-7636
- J4-4165
- J4-8228
- J4-9302
- CA15110
- CA15109
Public web page: http://www.nib.si/eng/index.php/systems-biology
Organisms: Potato virus y, Solanum tuberosum
FAIRDOM PALs: No PALs for this Project
Project start date: 1st Dec 2017
Project end date: 1st Jan 2020
Related items
- People (9)
- Programmes (1)
- Institutions (3)
- Investigations (1)
- Studies (5)
- Assays (23)
- Data files (338+16)
- Publications (1)
- Documents (120+75)
Projects: HYp - Spatiotemporal analysis of hypersensitive response to Potato virus Y in potato, pISA-tree, INDIE - Biotechnological production of sustainable indole, _p_stRT, Public project, Playground, SUSPHIRE - Sustainable Bioproduction of Pheromones for Insect Pest Control in Agriculture
Institutions: National Institute of Biology, NewHorizon
https://orcid.org/0000-0001-7484-6031Projects: HYp - Spatiotemporal analysis of hypersensitive response to Potato virus Y in potato, pISA-tree, MOA - Multiomics analysis of potato response to Potato virus Y (PVY) infection, SUSPHIRE - Sustainable Bioproduction of Pheromones for Insect Pest Control in Agriculture, INDIE - Biotechnological production of sustainable indole, FAIRDOM user meeting, _p_stRT, ADAPT - Accelerated Development of multiple-stress tolerAnt PoTato
Institutions: National Institute of Biology
https://orcid.org/0000-0001-5906-8569PhD in Biotechnology, Research Associate at Department of Biotechnology and Systems Biology, National Institute of Biology
Projects: _p_stRT
Institutions: Indiana University Bloomington
Projects: _p_stRT
Institutions: National Institute of Biology
Projects: FAIRDOM, FAIRDOM Community Workers, _p_stRT
Institutions: University of Manchester - Department of Computer Science, Manchester Centre for Integrative Systems Biology, University of Manchester
https://orcid.org/0000-0003-3156-2105Expertise: Synthetic Biology
Tools: Workflows, Web services
Projects: pISA-tree, HYp - Spatiotemporal analysis of hypersensitive response to Potato virus Y in potato, INDIE - Biotechnological production of sustainable indole, _p_stRT, ADAPT - Accelerated Development of multiple-stress tolerAnt PoTato, tst, tst2
Institutions: National Institute of Biology, tst
https://orcid.org/0000-0002-1669-6482Expertise: Molecular Biology, Statistics, Bioinformatics, Mathematical and statistical modeling, Programming, Data analysisMathematical modellingBioinformaticsSystems biology, Data Management, Data analysis, Visualization, Data Integration, Computational Biology
Tools: Bioinformatics, Computational and theoretical biology, Computational Systems Biology, Data Management, Databases, Dynamic modelling, Molecular Biology, Python, R, Systems Biology, Data Integration
Computational Biologist and Biostatistician at Department of Biotechnology and Systems Biology, National Institute of Biology (NIB)
National Institute of Biology, Department of Biotechnology and Systems Biology projects
Projects: HYp - Spatiotemporal analysis of hypersensitive response to Potato virus Y in potato, pISA-tree, MOA - Multiomics analysis of potato response to Potato virus Y (PVY) infection, SUSPHIRE - Sustainable Bioproduction of Pheromones for Insect Pest Control in Agriculture, INDIE - Biotechnological production of sustainable indole, _p_stRT, ADAPT - Accelerated Development of multiple-stress tolerAnt PoTato, _p_RNAinVAL, tst, tst2, Playground
Web page: http://www.nib.si/eng/index.php/departments/department-of-biotechnology-and-systems-biology
Investigation: _I_STRT Short Name: STRT Title: Cultivar-specific transcriptome and pan-transcriptome reconstruction of tetraploid potato Description: Cultivar-specific transcriptome and pan-transcriptome reconstruction of tetraploid potato Phenodata: ./phenodata_20191022.txt pISA Investigation creation date: 2019-10-22 pISA Investigation creator: Maja Zagorscak, Ziva Ramsak, Marko Petek Principal investigator: Kristina Gruden License: MIT Sharing permission: Public Upload to FAIRDOMHub: Yes
RELATED ...
Submitter: Maja Zagorscak
Studies: SupplementaryInformation, _S_01_sequences, _S_02_denovo, _S_03_stCuSTr, _S_04_stPanTr
Assays: Supplementary Information, _A_01_GC_content-count, _A_01_evigene, _A_02.1_BUSCO, _A_02.2_assembly-contribution-count, _A_02.3_InterProScan, _A_02.4_STAR, _A_02.5_STARlong_matchAnnot, _A_02.6_TransRate, _A_02.7_VecScreen, _A_02.8_DIAMOND, _A_02_cdhit_3cvs-GFFmerged, _A_03.1_filtering, _A_03.2_components, _A_03_components_3cvs-GFFmerged, _A_04_BUSCO_3cvs-GFFmerged, _A_04_TransRate, _A_05_BUSCO, _A_05_MSA_3cvs-GFFmerged, _A_06_tr_rep-transrate, _A_07_Desiree-mapping, _A_08_centrifuge_3cvs-GFFmerged, _A_09_annotation-GFFmerged
Snapshots: No snapshots
Short Name: 04_stPanTr Title: Solanum tuberosum Pan-Transcriptome Description: Solanum tuberosum Pan-Transcriptome Raw Data: pISA Study creation date: 2019-10-22 pISA Study creator: Maja Zagorscak Principal investigator: Kristina Gruden License: CC BY 4.0 Sharing permission: Public Upload to FAIRDOMHub: Yes
Submitter: Andrej Blejec
Investigation: _I_STRT
Assays: _A_01_GC_content-count, _A_02_cdhit_3cvs-GFFmerged, _A_03_components_3cvs-GFFmerged, _A_04_BUSCO_3cvs-GFFmerged, _A_05_MSA_3cvs-GFFmerged, _A_06_tr_rep-transrate, _A_07_Desiree-mapping, _A_08_centrifuge_3cvs-GFFmerged, _A_09_annotation-GFFmerged
Snapshots: No snapshots
Supplementary Information linked together
Snapshots: No snapshots
Study: _S_03_stCuSTr Short Name: 03_stCuSTr Title: Solanum tuberosum Cultivar-Specific Transcriptomes Description: Solanum tuberosum Cultivar-Specific Transcriptomes Raw Data: ../_S_01_sequences/, ../_S_02_denovo/ pISA Study creation date: 2019-10-22 pISA Study creator: Maja Zagorscak, Marko Petek, Ziva Ramsak Principal investigator: Kristina Gruden License: MIT Sharing permission: Public Upload to FAIRDOMHub: Yes
RELATED FILES: /reports/README.MD https://fairdomhub.org/documents/346 /_STUDY_METADATA.TXT ...
Submitter: Maja Zagorscak
Investigation: _I_STRT
Assays: _A_01_evigene, _A_02.1_BUSCO, _A_02.2_assembly-contribution-count, _A_02.3_InterProScan, _A_02.4_STAR, _A_02.5_STARlong_matchAnnot, _A_02.6_TransRate, _A_02.7_VecScreen, _A_02.8_DIAMOND, _A_03.1_filtering, _A_03.2_components, _A_04_TransRate, _A_05_BUSCO
Snapshots: No snapshots
Study: _S_02_denovo Short Name: 02_denovo Title: De novo assemblies Description: De novo assemblies for cration of potato reference transcriptomes Raw Data: ../_S_01_sequences/ pISA Study creation date: 2019-10-23 pISA Study creator: Maja Zagorscak, Marko Petek, Ziva Ramsak Principal investigator: Kristina Gruden License: MIT Sharing permission: Public Upload to FAIRDOMHub: Yes
RELATED FILES: /reports/README.MD https://fairdomhub.org/documents/343 /_STUDY_METADATA.TXT https://fairdomhub.org/documents/342 ...
Snapshots: Snapshot 1
Study: _S_01_sequences Short Name: 01_sequences Title: NGS sequences for de novo assembly Description: NGS sequences for de novo assembly; input for Study 02_denovo Raw Data: see: ./reports/SupplementaryTableS1-Input_sequences_information.xlsx pISA Study creation date: 2019-10-23 pISA Study creator: Maja Zagorscak, Marko Petek, Ziva Ramsak Principal investigator: Kristina Gruden License: MIT Sharing permission: Public Upload to FAIRDOMHub: Yes
RELATED FILES: /reports/README.MD ...
Snapshots: Snapshot 1
Assay: _A_01_evigene Short Name: 01_evigene Assay Class: DRY Assay Type: evigene Title: EvidentialGene tr2aacds.pl VERSION 2016.07.11 Description: See http://eugenes.org/EvidentialGene/about/EvidentialGene_trassembly_pipe.html pISA Assay creation date: 2019-10-22 pISA Assay creator: Maja Zagorscak Phenodata: None Featuredata: None Data: see ./input/path_to_files.txt
Submitter: Maja Zagorscak
Biological problem addressed: Integration
Investigation: _I_STRT
Study: _S_03_stCuSTr
Organisms: Solanum tuberosum, Potato virus Y
Models: No Models
SOPs: No SOPs
Data files: /input/path_to_files.txt, /intermediate_tr2aacds_Desiree.tar.gz, /intermediate_tr2aacds_PW363.tar.gz.aa, /intermediate_tr2aacds_PW363.tar.gz.ab, /intermediate_tr2aacds_Rywal.tar.gz, /output/output.tar, /scripts/01_run_commands.sh, /scripts/my_tr2aacds.sh, /scripts/run_tr2aacds.sh
Snapshots: Snapshot 1
Assay: _A_02.1_BUSCO Short Name: 02.1_BUSCO Assay Class: DRY Assay Type: BUSCO Title: BUSCO on assemblies and tr2aacds output Description: BUSCO on assemblies and tr2aacds output pISA Assay creation date: 2019-10-22 pISA Assay creator: Maja Zagorscak Phenodata: None Featuredata: None Data: see ./input/path_to_files.txt
Submitter: Maja Zagorscak
Biological problem addressed: Validation
Investigation: _I_STRT
Study: _S_03_stCuSTr
Organisms: Solanum tuberosum
Models: No Models
SOPs: No SOPs
Data files: /input/input.tar, /output/output.tar, /scripts/scripts.tar
Snapshots: Snapshot 1
Assay: _A_02.2_assembly-contribution-count Short Name: 02.2_assembly-contribution-count Assay Class: DRY Assay Type: count Title: assembly contribution count Description: assembly contribution count, input/output tr2aacds pISA Assay creation date: 2019-10-22 pISA Assay creator: Maja Zagorscak Phenodata: None Featuredata: None Data: see ./input/path_to_files.txt
Submitter: Maja Zagorscak
Biological problem addressed: Model Analysis Type
Investigation: _I_STRT
Study: _S_03_stCuSTr
Organisms: Solanum tuberosum
Models: No Models
SOPs: No SOPs
Data files: /input/input.tar, /intermediate_Desiree.tar.gz, /intermediate_PW363.tar.gz, /intermediate_Rywal.tar.gz, /output/output.tar, /reports/SupplementaryFigure1.pdf, /reports/SupplementaryFigure2.pdf, /reports/SupplementaryFigure3.pdf, /reports/SupplementaryTableS6, /scripts/scripts.tar
Snapshots: Snapshot 1
Assay: _A_02.3_InterProScan Short Name: 02.3_InterProScan Assay Class: DRY Assay Type: InterProScan Title: InterProScan on tr2aacds output Description: InterProScan on tr2aacds output pISA Assay creation date: 2019-10-22 pISA Assay creator: Marko Petek Phenodata: None Featuredata: None Data: see ./input/path_to_files.txt
Submitter: Maja Zagorscak
Biological problem addressed: Annotation
Investigation: _I_STRT
Study: _S_03_stCuSTr
Organisms: Solanum tuberosum
Models: No Models
SOPs: No SOPs
Data files: /input/path_to_files.txt, /intermediate/intermediate.tar, /output/Desiree_IPS_filtered_aggregated_filtere..., /output/PW363_IPS_filtered_aggregated_filtered.tsv, /output/Rywal_IPS_filtered_aggregated_filtered.tsv, /reports/IPS_PowerQuery_PivotTable.xlsx, /scripts/01_potato_tr_evigene_IPS_commands.txt, /scripts/02_reshape_merge_aggregate_filter.Rmd
Snapshots: Snapshot 1
Assay: _A_02.4_STAR Short Name: 02.4_STAR Assay Class: DRY Assay Type: STAR Title: mapping reads back to evigene .tr output Description: mapping reads back to evigene .tr output using STAR to check the percentage of reads that map pISA Assay creation date: 2019-10-22 pISA Assay creator: Marko Petek Phenodata: None Featuredata: None Data: see ./input/path_to_files.txt
Submitter: Maja Zagorscak
Biological problem addressed: Validation
Investigation: _I_STRT
Study: _S_03_stCuSTr
Organisms: Solanum tuberosum
Models: No Models
SOPs: No SOPs
Data files: /input/path_to_files.txt, /output/output_STAR_logs-n-SJ.tar.gz, /output/samtoolsCoverage.zip, /reports/summaryCounts&SJ.xlsx, /scripts/STAR_commands.txt
Snapshots: Snapshot 1
Assay: _A_02.5_STARlong_matchAnnot Short Name: 02.5_STARlong_matchAnnot Assay Class: DRY Assay Type: matchAnnot Title: potato transcriptome v2 (evigene) STARlong mapping of transcripts to reference genome and MatchAnnot to get transcript annotations Description: potato initial transcriptome (evigene) STARlong mapping of transcripts to reference genome and MatchAnnot to get transcript annotations pISA Assay creation date: 2019-10-22 pISA Assay creator: Marko Petek Phenodata: None Featuredata: None ...
Submitter: Maja Zagorscak
Biological problem addressed: Annotation
Investigation: _I_STRT
Study: _S_03_stCuSTr
Organisms: Solanum tuberosum
Models: No Models
SOPs: No SOPs
Data files: /input/path_to_files.txt, /output/output_1_STARlong-logs-n-SJ.tar.gz, /output/output_2_matchAnnot-parsed-txt.tar.gz, /scripts/STARlongMatchAnnot_commands_ENCODE_den...
Snapshots: Snapshot 1
Assay: _A_02.6_TransRate Short Name: 02.6_TransRate Assay Class: DRY Assay Type: TransRate Title: TransRate stat on raw tr2aacds transcriptomes Description: TransRate stat on raw (initial) tr2aacds transcriptomes, basic and reference-based pISA Assay creation date: 2019-10-22 pISA Assay creator: Maja Zagorscak Phenodata: None Featuredata: Nonde Data: see ./input/path_to_files.txt
Submitter: Maja Zagorscak
Biological problem addressed: Validation
Investigation: _I_STRT
Study: _S_03_stCuSTr
Organisms: Solanum tuberosum
Models: No Models
SOPs: No SOPs
Data files: /input/input.tar, /output/output.tar, /scripts/scripts.tar
Snapshots: Snapshot 1
Assay: _A_02.7_VecScreen Short Name: 02.7_VecScreen Assay Class: DRY Assay Type: VecScreen Title: VecScreen on raw tr2aacds output Description: VecScreen (contamination screening) and blastn (contaminants annotation) on raw (initial) tr2aacds output pISA Assay creation date: 2019-10-22 pISA Assay creator: Maja Zagorscak Phenodata: None Featuredata: None Data: see ./input/path_to_files.txt
Submitter: Maja Zagorscak
Biological problem addressed: Validation
Investigation: _I_STRT
Study: _S_03_stCuSTr
Organisms: Solanum tuberosum, Potato virus Y
Models: No Models
SOPs: No SOPs
Data files: /input/path_to_files.txt, /intermediate.tar.gz, /output/Desiree_vecscreen.tsv, /output/PW363_vecscreen.tsv, /output/Rywal_vecscreen.tsv, /scripts/01_get_input.sh, /scripts/02_VecScreenPlus_blastn.sh, /scripts/ENCH_sumablastplus.pl
Snapshots: Snapshot 1
Assay: _A_02.8_DIAMOND Short Name: 02.8_DIAMOND Assay Class: DRY Assay Type: DIAMOND Title: DIAMOND on raw tr2aacds output Description: DIAMOND on raw tr2aacds output, both .cds and .tr; including lost and found pISA Assay creation date: 2019-10-22 pISA Assay creator: Maja Zagorscak Phenodata: None Featuredata: None Data: see ./input/path_to_files.txt
Submitter: Maja Zagorscak
Biological problem addressed: Annotation
Investigation: _I_STRT
Study: _S_03_stCuSTr
Organisms: Solanum tuberosum
Models: No Models
SOPs: No SOPs
Data files: /input/input.tar, /output/output.tar, /scripts/scripts.tar
Snapshots: Snapshot 1
Assay: _A_03.1_filtering Short Name: 03.1_filtering Assay Class: DRY Assay Type: filtering Title: Filtering according to biological evidence; removal of contaminants, chimeras and suspicious constructs Description: Filtering according to biological evidence; removal of contaminants, chimeras and suspicious constructs pISA Assay creation date: 2019-10-22 pISA Assay creator: Maja Zagorscak Phenodata: None Featuredata: None Data: see ./input/path_to_files.txt
Submitter: Maja Zagorscak
Biological problem addressed: Validation
Investigation: _I_STRT
Study: _S_03_stCuSTr
Organisms: No organisms
Models: No Models
SOPs: No SOPs
Data files: /input/input.tar, /intermediate/intermediate.tar, /output/Desiree_tr.cds.tsv.gz, /output/PW363_tr.cds.tsv.gz, /output/Rywal_tr.cds.tsv.gz, /output/other/output_full.tar.gz, /output/other/output_summary.tar.gz, /reports/03.1_Rywal_combo_withFiltering.html, /reports/03.2_PW363_combo_withFiltering.html, /reports/03.3_Desiree_combo_withFiltering.html, /reports/SupplementaryTableS3, /reports/SupplementaryTableS4, /reports/SupplementaryTableS5, /scripts/scripts.tar
Snapshots: Snapshot 1
Assay: _A_03.2_components Short Name: 03.2_components Assay Class: DRY Assay Type: components Title: Components: tr2aacds headers, cdhit-2d Description: Components: tr2aacds headers, cdhit-2d; post-filtering redefinition of paralogue clusters pISA Assay creation date: 2019-10-22 pISA Assay creator: Ziva Ramsak Phenodata: None Featuredata: Data:
Submitter: Maja Zagorscak
Biological problem addressed: Annotation
Investigation: _I_STRT
Study: _S_03_stCuSTr
Organisms: Solanum tuberosum
Models: No Models
SOPs: No SOPs
Data files: /input/input.tar, /intermediate_4_cdhit-2d.tar.gz, /output/output.tar, /scripts/scripts.tar
Snapshots: Snapshot 1
Assay: _A_04_TransRate Short Name: 04_TransRate Assay Class: DRY Assay Type: TransRate Title: TransRate post filtering and reassignment Description: TransRate post filtering and reassignment pISA Assay creation date: 2019-10-22 pISA Assay creator: Maja Zagorscak Phenodata: None Featuredata: None Data: see ./input/path_to_files.txt
Submitter: Maja Zagorscak
Biological problem addressed: Validation
Investigation: _I_STRT
Study: _S_03_stCuSTr
Organisms: Solanum tuberosum
Models: No Models
SOPs: No SOPs
Data files: /input/path_to_files.txt, /output/output.tar, /scripts/run_TransRate_commands.sh
Snapshots: Snapshot 1
Assay: _A_05_BUSCO Short Name: 05_BUSCO Assay Class: DRY Assay Type: BUSCO Title: BUSCO post filtering and reassignment Description: BUSCO post filtering and reassignment pISA Assay creation date: 2019-10-22 pISA Assay creator: Maja Zagorscak Phenodata: None Featuredata: None Data: see ./input/path_to_files.txt
Submitter: Maja Zagorscak
Biological problem addressed: Validation
Investigation: _I_STRT
Study: _S_03_stCuSTr
Organisms: Solanum tuberosum
Models: No Models
SOPs: No SOPs
Data files: /input/input.tar, /output/output.tar, /scripts/scripts.tar, /transfer/transfer.zip
Snapshots: Snapshot 1
Supplementary Information linked together
Submitter: Maja Zagorscak
Biological problem addressed: Model Analysis Type
Investigation: _I_STRT
Study: SupplementaryInformation
Organisms: No organisms
Models: No Models
SOPs: No SOPs
Data files: /reports/SupplementaryFigure1.pdf, /reports/SupplementaryFigure2.pdf, /reports/SupplementaryFigure3.pdf, /reports/SupplementaryFigure4.pdf, /reports/SupplementaryFile2.html, /reports/SupplementaryFile3.html, /reports/SupplementaryTableS1, /reports/SupplementaryTableS2, /reports/SupplementaryTableS3, /reports/SupplementaryTableS4, /reports/SupplementaryTableS5, /reports/SupplementaryTableS6, /reports/SupplementaryTableS7, /reports/SupplementaryTableS8, SupplementaryFile1
Snapshots: Snapshot 1
Short Name: cdhit_3cvs-GFFmerged Assay Class: DRY Assay Type: GFFmerged Title: cdhit-est and cdhit-2d on 3cv and ITAG/PGSC Description: cdhit-est and cdhit-2d on 3cv and ITAG/PGSC pISA Assay creation date: 2020-03-09 pISA Assay creator: Maja Zagorscak Phenodata: None Featuredata: Data:
Submitter: Andrej Blejec
Biological problem addressed: Model Analysis Type
Investigation: _I_STRT
Study: _S_04_stPanTr
Organisms: No organisms
Models: No Models
SOPs: No SOPs
Data files: /input/Solanum_tuberosum_ITAG_DM_v1_description..., /input/Solanum_tuberosum_PGSC_DM_v4.04_converte..., /input/StPGSC4.04n_seq_4_PGSC-p-rep_ITAG-pep_gf..., /input/path_to_files.txt, /intermediate/singletons.DM.seqID.txt, /intermediate/singletons.seqID.txt, /intermediate/singletons.tetra.seqID.txt, /intermediate/stCuSTr_cds_repAlt_cdhitEST-conda..., /intermediate/stPanTr_aa_singletons_DM_on_tetra..., /intermediate/stPanTr_aa_singletons_tetra_on_DM..., /output/5cv_weak-components.txt, /output/5cv_weak-components_withAnnotation.tsv, /output/VennDiagrams/stPanTr+stCuSTr_geneCount_..., /output/VennDiagrams/stPanTr_geneCount_weak-com..., /output/VennDiagrams/stPanTr_geneCount_weak-com..., /output/VennDiagrams/stPanTr_geneCount_weak-com..., /output/VennDiagrams/stPanTr_geneCount_weak-com..., /output/VennDiagrams/stPanTr_geneCount_weak-com..., /output/VennDiagrams/stPanTr_geneCount_weak-com..., /output/VennDiagrams/stPanTr_geneCount_weak-com..., /output/VennDiagrams/stPanTr_geneCount_weak-com..., /output/annot.alias.aa.tsv, /output/annot.alias.cds.tsv, /output/cdhit-est_rep-alt_aS.75_c.90_pairs.tsv, /output/cdhit-est_representativesMatched_aS.75_..., /output/cdhit-est_singletons_aS.75_c.90.tsv, /output/stPanTr-clusters.tsv, /output/trueSingle.DM.ID, /output/trueSingle.polyploid.ID, /reports/SupplementaryFigure4.pdf, /reports/SupplementaryTableS7, /reports/cvID_group-Rplot.pdf, /reports/cv_group-Rplot.pdf, /scripts/01.1_commands-CDHIT-EST.txt, /scripts/01.2_commands-CDHIT-2D.txt, /scripts/02_cd-hit-est_cdhit-2d.html, /scripts/03_stPanTr_components_all.html, /scripts/04_summary_stPanTr_v4.html and 4 hidden items
Snapshots: Snapshot 1, Snapshot 2
Short Name: 01_GC_content-count Assay Class: DRY Assay Type: count Title: GC content and sequence length Description: A, C, T, G content and sequence length pISA Assay creation date: 2020-03-09 pISA Assay creator: Maja Zagorscak Phenodata: None Featuredata: Data: ../input/path_to_files.txt
Submitter: Andrej Blejec
Biological problem addressed: Model Analysis Type
Investigation: _I_STRT
Study: _S_04_stPanTr
Organisms: No organisms
Models: No Models
SOPs: No SOPs
Data files: /input/Solanum_tuberosum-ITAG_DM_v1_cds.fasta, /input/Solanum_tuberosum_PGSC_merged.fasta, /input/StPGSC4.04n_seq_3_PGSC-c-rep_ITAG-cds_gf..., /output/Solanum_tuberosum-ITAG_DM_v1_cds_GC.txt, /output/Solanum_tuberosum_PGSC_merged.CDS_GC.txt, /output/StPGSC4.04n_seq_3_PGSC-c-rep_ITAG-cds_g..., /scripts/GC_content.py, /scripts/tab_to_fasta.py and 12 hidden items
Snapshots: No snapshots
Short Name: 04_BUSCO_3cvs-GFFmerged Assay Class: DRY Assay Type: GFFmerged Title: BUSCOs Description: BUSCO on ITAG, PGSC, ITAG/PGSC merged, pan-transcriptome representatives, pan-transcriptome alternatives, and pan-transcriptome pISA Assay creation date: 2020-03-10 pISA Assay creator: Maja Zagorscak Phenodata: None Featuredata: Data:
Submitter: Andrej Blejec
Biological problem addressed: Model Analysis Type
Investigation: _I_STRT
Study: _S_04_stPanTr
Organisms: No organisms
Models: No Models
SOPs: No SOPs
Data files: /output/BUSCO_stPanTr_embryophyta_odb9.tsv, /output/BUSCO_stPanTr_solanaceae_odb10.tsv, /output/short_summary_BUSCO_Solanum_tuberosum-I..., /output/short_summary_BUSCO_Solanum_tuberosum-I..., /output/short_summary_BUSCO_Solanum_tuberosum-P..., /output/short_summary_BUSCO_Solanum_tuberosum-P..., /output/short_summary_BUSCO_Solanum_tuberosum_P..., /output/short_summary_BUSCO_Solanum_tuberosum_P..., /output/short_summary_BUSCO_Solanum_tuberosum_P..., /output/short_summary_BUSCO_Solanum_tuberosum_P..., /output/short_summary_BUSCO_StPGSC4.04n_seq_4_P..., /output/short_summary_BUSCO_StPGSC4.04n_seq_4_P..., /output/short_summary_BUSCO_stPanTr.aa.fasta_em..., /output/short_summary_BUSCO_stPanTr.aa.fasta_so..., /output/short_summary_BUSCO_stPanTr_alt.aa.fast..., /output/short_summary_BUSCO_stPanTr_alt.aa.fast..., /output/short_summary_BUSCO_stPanTr_rep.aa.fast..., /output/short_summary_BUSCO_stPanTr_rep.aa.fast..., /scripts/01_run_BUSCO_commands.sh, /scripts/02_BUSCO_plots.html
Snapshots: No snapshots
Short Name: 07_Desiree-mapping Assay Class: DRY Assay Type: mapping Title: Desiree mapping Description: Mapping of Desiree samples (drought stress) to Desiree and Phureja transcriptomes pISA Assay creation date: 2020-03-11 pISA Assay creator: Maja Zagorscak Phenodata: None Featuredata: Data:
Submitter: Andrej Blejec
Biological problem addressed: Model Analysis Type
Investigation: _I_STRT
Study: _S_04_stPanTr
Organisms: No organisms
Models: No Models
SOPs: No SOPs
Data files: /input/PGSC_DM_v3.4_transcript-update_represent..., /intermediate/Desiree/chrLength.txt, /intermediate/Desiree/chrName.txt, /intermediate/Desiree/chrNameLength.txt, /intermediate/Desiree/chrStart.txt, /intermediate/Desiree/genomeParameters.txt, /intermediate/PGSC/chrLength.txt, /intermediate/PGSC/chrName.txt, /intermediate/PGSC/chrNameLength.txt, /intermediate/PGSC/chrStart.txt, /intermediate/PGSC/genomeParameters.txt, /intermediate/gff-merged/chrLength.txt, /intermediate/gff-merged/chrName.txt, /intermediate/gff-merged/chrNameLength.txt, /intermediate/gff-merged/chrStart.txt, /intermediate/gff-merged/genomeParameters.txt, /intermediate/indexDesiree.log, /intermediate/indexGFFmerged.log, /intermediate/indexPGSC.log, /output/Desiree_SRR10416847/Log.final.out, /output/Desiree_SRR10416847/Log.out, /output/Desiree_SRR10416847/Log.progress.out, /output/Desiree_SRR10416847/SJ.out.tab, /output/Desiree_SRR10416848/Log.final.out, /output/Desiree_SRR10416848/Log.out, /output/Desiree_SRR10416848/Log.progress.out, /output/Desiree_SRR10416848/SJ.out.tab, /output/Desiree_SRR10416849/Log.final.out, /output/Desiree_SRR10416849/Log.out, /output/Desiree_SRR10416849/Log.progress.out, /output/Desiree_SRR10416849/SJ.out.tab, /output/Desiree_drought123/Desiree_Aligned.sort..., /output/Desiree_drought123/Log.final.out, /output/Desiree_drought123/Log.out, /output/Desiree_drought123/Log.progress.out, /output/Desiree_drought123/SJ.out.tab, /output/PGSC_SRR10416847/Log.final.out, /output/PGSC_SRR10416847/Log.out, /output/PGSC_SRR10416847/Log.progress.out, /output/PGSC_SRR10416847/SJ.out.tab, /output/PGSC_SRR10416848/Log.final.out, /output/PGSC_SRR10416848/Log.out, /output/PGSC_SRR10416848/Log.progress.out, /output/PGSC_SRR10416848/SJ.out.tab, /output/PGSC_SRR10416849/Log.final.out, /output/PGSC_SRR10416849/Log.out, /output/PGSC_SRR10416849/Log.progress.out, /output/PGSC_SRR10416849/SJ.out.tab, /output/PGSC_drought123/DM_Aligned.sortedByCoor..., /output/PGSC_drought123/Log.final.out, /output/PGSC_drought123/Log.out, /output/PGSC_drought123/Log.progress.out, /output/PGSC_drought123/SJ.out.tab, /output/gff-merged_SRR10416847/Log.final.out, /output/gff-merged_SRR10416847/Log.out, /output/gff-merged_SRR10416847/Log.progress.out, /output/gff-merged_SRR10416847/SJ.out.tab, /output/gff-merged_SRR10416848/Log.final.out, /output/gff-merged_SRR10416848/Log.out, /output/gff-merged_SRR10416848/Log.progress.out, /output/gff-merged_SRR10416848/SJ.out.tab, /output/gff-merged_SRR10416849/Log.final.out, /output/gff-merged_SRR10416849/Log.out, /output/gff-merged_SRR10416849/Log.progress.out, /output/gff-merged_SRR10416849/SJ.out.tab, /output/gff-merged_drought123/Log.final.out, /output/gff-merged_drought123/Log.out, /output/gff-merged_drought123/Log.progress.out, /output/gff-merged_drought123/SJ.out.tab, /reports/SupplementaryTableS8, /scripts/00_wget.txt, /scripts/01_index_ref.txt, /scripts/02_map.txt, /scripts/03_clean-dir.txt
Snapshots: No snapshots
Short Name: 09_annotation-GFFmerged Assay Class: DRY Assay Type: GFFmerged Title: Pan-transcriptome fasta files annotation Description: Annotation of pan-transcriptome and re-annotation of cultivar-specific transcriptomes pISA Assay creation date: 2020-03-11 pISA Assay creator: Maja Zagorscak Phenodata: None Featuredata: Data:
Submitter: Andrej Blejec
Biological problem addressed: Model Analysis Type
Investigation: _I_STRT
Study: _S_04_stPanTr
Organisms: No organisms
Models: No Models
SOPs: No SOPs
Data files: /input/path_to_files.txt, /output/annot_stCuSTr-D_aa_all.zip, /output/annot_stCuSTr-D_aa_representatives.zip, /output/annot_stCuSTr-D_cds_all.zip, /output/annot_stCuSTr-D_cds_representatives.zip, /output/annot_stCuSTr-D_tr_all.zip, /output/annot_stCuSTr-D_tr_representatives.zip, /output/annot_stCuSTr-P_aa_all.zip, /output/annot_stCuSTr-P_aa_representatives.zip, /output/annot_stCuSTr-P_cds_all.zip, /output/annot_stCuSTr-P_cds_representatives.zip, /output/annot_stCuSTr-P_tr_all.zip, /output/annot_stCuSTr-P_tr_representatives.zip, /output/annot_stCuSTr-R_aa_all.zip, /output/annot_stCuSTr-R_aa_representatives.zip, /output/annot_stCuSTr-R_cds_all.zip, /output/annot_stCuSTr-R_cds_representatives.zip, /output/annot_stCuSTr-R_tr_all.zip, /output/annot_stCuSTr-R_tr_representatives.zip, /output/annot_stPanTr.aa.zip, /output/annot_stPanTr.cds.zip, /output/annot_stPanTr_rep.aa.zip, /output/annot_stPanTr_rep.cds.zip, /scripts/annotate.sh
Snapshots: Snapshot 1, Snapshot 2
Short Name: 03_components_3cvs-GFFmerged Assay Class: DRY Assay Type: GFFmerged Title: components Description: representative and alternative pan-transcriptome CDS and polypeptide fasta files pISA Assay creation date: 2020-03-10 pISA Assay creator: Maja Zagorscak Phenodata: None Featuredata: Data:
Submitter: Andrej Blejec
Biological problem addressed: Model Analysis Type
Investigation: _I_STRT
Study: _S_04_stPanTr
Organisms: No organisms
Models: No Models
SOPs: No SOPs
Data files: /input/path_to_files.txt, /output/Solanum_tuberosum_PGSC_DM_v4.04_CDS-to-..., /output/alt.aa.IDs, /output/alt.cds.IDs, /output/rep.aa.IDs, /output/rep.cds.IDs, /scripts/commands-components_1_3cvs-gffmerged.txt
Snapshots: No snapshots
coverage and uniquely mapped spliced reads summary
samtools coverage https://www.htslib.org/doc/samtools-coverage.html
Creator: Maja Zagorscak
Submitter: Maja Zagorscak
_p_stRT/_I_STRT/_S_04_stPanTr/_A_09_annotation-GFFmerged
Creator: Andrej Blejec
Submitters: Andrej Blejec, Maja Zagorscak
_p_stRT/_I_STRT/_S_04_stPanTr/_A_09_annotation-GFFmerged
Creator: Andrej Blejec
Submitters: Andrej Blejec, Maja Zagorscak
_p_stRT/_I_STRT/_S_04_stPanTr/_A_09_annotation-GFFmerged
Creator: Andrej Blejec
Submitters: Andrej Blejec, Maja Zagorscak
_p_stRT/_I_STRT/_S_04_stPanTr/_A_09_annotation-GFFmerged
Creator: Andrej Blejec
Submitters: Andrej Blejec, Maja Zagorscak
_p_stRT/_I_STRT/_S_04_stPanTr/_A_09_annotation-GFFmerged
Creator: Andrej Blejec
Submitters: Andrej Blejec, Maja Zagorscak
_p_stRT/_I_STRT/_S_04_stPanTr/_A_09_annotation-GFFmerged
Creator: Andrej Blejec
Submitters: Andrej Blejec, Maja Zagorscak
_p_stRT/_I_STRT/_S_04_stPanTr/_A_09_annotation-GFFmerged
Creator: Andrej Blejec
Submitters: Andrej Blejec, Maja Zagorscak
_p_stRT/_I_STRT/_S_04_stPanTr/_A_09_annotation-GFFmerged
Creator: Andrej Blejec
Submitters: Andrej Blejec, Maja Zagorscak
_p_stRT/_I_STRT/_S_04_stPanTr/_A_09_annotation-GFFmerged
Creator: Andrej Blejec
Submitters: Andrej Blejec, Maja Zagorscak
_p_stRT/_I_STRT/_S_04_stPanTr/_A_09_annotation-GFFmerged
Creator: Andrej Blejec
Submitters: Andrej Blejec, Maja Zagorscak
_p_stRT/_I_STRT/_S_04_stPanTr/_A_09_annotation-GFFmerged
Creator: Andrej Blejec
Submitters: Andrej Blejec, Maja Zagorscak
_p_stRT/_I_STRT/_S_04_stPanTr/_A_09_annotation-GFFmerged
Creator: Andrej Blejec
Submitters: Andrej Blejec, Maja Zagorscak
_p_stRT/_I_STRT/_S_04_stPanTr/_A_09_annotation-GFFmerged
Creator: Andrej Blejec
Submitters: Andrej Blejec, Maja Zagorscak
_p_stRT/_I_STRT/_S_04_stPanTr/_A_09_annotation-GFFmerged
Creator: Andrej Blejec
Submitters: Andrej Blejec, Maja Zagorscak
_p_stRT/_I_STRT/_S_04_stPanTr/_A_09_annotation-GFFmerged
Creator: Andrej Blejec
Submitters: Andrej Blejec, Maja Zagorscak
_p_stRT/_I_STRT/_S_04_stPanTr/_A_09_annotation-GFFmerged
Creator: Andrej Blejec
Submitters: Andrej Blejec, Maja Zagorscak
_p_stRT/_I_STRT/_S_04_stPanTr/_A_09_annotation-GFFmerged
Creator: Andrej Blejec
Submitters: Andrej Blejec, Maja Zagorscak
_p_stRT/_I_STRT/_S_04_stPanTr/_A_09_annotation-GFFmerged
Creator: Andrej Blejec
Submitters: Andrej Blejec, Maja Zagorscak
Abstract (Expand)
Authors: Marko Petek, Maja Zagorščak, Živa Ramšak, Sheri Sanders, Elizabeth Tseng, Mohamed Zouine, Anna Coll, Kristina Gruden
Date Published: No date defined
Publication Type: Not specified
DOI: 10.1101/845818
Citation: Cultivar-specific transcriptome and pan-transcriptome reconstruction of tetraploid potato
Creator: Sheri Sanders
Submitter: Maja Zagorscak
Investigations: No Investigations
Studies: No Studies
Assays: No Assays
Description of Tags found on transcript headers https://blogs.iu.edu/ncgas/2018/12/17/how-evigene-works/#step3
Creator: Sheri Sanders
Submitter: Maja Zagorscak
_p_stRT/_I_STRT/_S_04_stPanTr/_A_02_cdhit_3cvs-GFFmerged
Creator: Andrej Blejec
Submitters: Andrej Blejec, Maja Zagorscak
_p_stRT/_I_STRT/_S_04_stPanTr/_A_02_cdhit_3cvs-GFFmerged
Creator: Andrej Blejec
Submitters: Andrej Blejec, Maja Zagorscak
_p_stRT/_I_STRT/_S_04_stPanTr/_A_02_cdhit_3cvs-GFFmerged
Creator: Andrej Blejec
Submitters: Andrej Blejec, Maja Zagorscak
_p_stRT/_I_STRT/_S_04_stPanTr/_A_02_cdhit_3cvs-GFFmerged
Creator: Andrej Blejec
Submitters: Andrej Blejec, Maja Zagorscak
_p_stRT/_I_STRT/_S_04_stPanTr/_A_02_cdhit_3cvs-GFFmerged
Creator: Andrej Blejec
Submitters: Andrej Blejec, Maja Zagorscak
_p_stRT/_I_STRT/_S_04_stPanTr/_A_02_cdhit_3cvs-GFFmerged
Creator: Andrej Blejec
Submitters: Andrej Blejec, Maja Zagorscak
_p_stRT/_I_STRT/_S_04_stPanTr/_A_02_cdhit_3cvs-GFFmerged
Creator: Andrej Blejec
Submitters: Andrej Blejec, Maja Zagorscak
_p_stRT/_I_STRT/_S_04_stPanTr/_A_02_cdhit_3cvs-GFFmerged
Creator: Andrej Blejec
Submitters: Andrej Blejec, Maja Zagorscak
_p_stRT/_I_STRT/_S_04_stPanTr/_A_02_cdhit_3cvs-GFFmerged
Creator: Andrej Blejec
Submitters: Andrej Blejec, Maja Zagorscak
_p_stRT/_I_STRT/_S_04_stPanTr/_A_02_cdhit_3cvs-GFFmerged
Creator: Andrej Blejec
Submitters: Andrej Blejec, Maja Zagorscak
_p_stRT/_I_STRT/_S_04_stPanTr/_A_02_cdhit_3cvs-GFFmerged
Creator: Andrej Blejec
Submitters: Andrej Blejec, Maja Zagorscak
_p_stRT/_I_STRT/_S_04_stPanTr/_A_02_cdhit_3cvs-GFFmerged
Creator: Andrej Blejec
Submitters: Andrej Blejec, Maja Zagorscak
_p_stRT/_I_STRT/_S_04_stPanTr/_A_02_cdhit_3cvs-GFFmerged
Creator: Andrej Blejec
Submitters: Andrej Blejec, Maja Zagorscak
_p_stRT/_I_STRT/_S_04_stPanTr/_A_02_cdhit_3cvs-GFFmerged
Creator: Andrej Blejec
Submitters: Andrej Blejec, Maja Zagorscak
_p_stRT/_I_STRT/_S_04_stPanTr/_A_02_cdhit_3cvs-GFFmerged
Creator: Andrej Blejec
Submitters: Andrej Blejec, Maja Zagorscak
Creator: Maja Zagorscak
Submitter: Maja Zagorscak
_p_stRT/_I_STRT/_S_04_stPanTr/_A_09_annotation-GFFmerged
Creator: Andrej Blejec
Submitter: Andrej Blejec
_p_stRT/_I_STRT/_S_04_stPanTr/_A_09_annotation-GFFmerged
Creator: Andrej Blejec
Submitter: Andrej Blejec