_A_02.4_STAR

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Assay: _A_02.4_STAR Short Name: 02.4_STAR Assay Class: DRY Assay Type: STAR Title: mapping reads back to evigene .tr output Description: mapping reads back to evigene .tr output using STAR to check the percentage of reads that map pISA Assay creation date: 2019-10-22 pISA Assay creator: Marko Petek Phenodata: None Featuredata: None Data: see ./input/path_to_files.txt

SEEK ID: https://fairdomhub.org/assays/1165

Class: Modelling analysis

Projects: _p_stRT

Investigation: _I_STRT

Study: _S_03_stCuSTr

Assay position:

Biological problem addressed: Validation

Organisms: Solanum tuberosum

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Creators and Submitter

Creators

Maja Zagorscak
Marko Petek

Submitter

Maja Zagorscak

Citation

Zagorscak, M., & Petek, M. (2019). _A_02.4_STAR. FAIRDOMHub. https://doi.org/10.15490/FAIRDOMHUB.1.ASSAY.1165.1

Note: This is a citation for Snapshot 1 of this Assay, the contents of which may vary from what is shown on this page.

Snapshots

Snapshot 1 (26th Nov 2019) DOI

Activity

Views: 1576

Created: 7th Nov 2019 at 16:48

Last updated: 2nd Jun 2020 at 13:42

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People (2)
Programmes (1)
Projects (1)
Investigations (1)
Studies (1)
Data files (5)
Publications (1)
Documents (2)

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Marko Petek

Projects: pISA-tree, SUSPHIRE - Sustainable Bioproduction of Pheromones for Insect Pest Control in Agriculture, _p_stRT, ADAPT - Accelerated Development of multiple-stress tolerAnt PoTato, _p_RNAinVAL

Institutions: National Institute of Biology

https://orcid.org/0000-0003-3644-7827

Expertise: Molecular Biology, Transcriptomics, Solanum tuberosum, Plant biology, plant pathways, plant metabolism, Systems Biology

Tools: Molecular biology techniques (RNA/DNA/Protein), Microarray analysis, Bioinformatics, R, RNAseq, RNA interference, metabolic network visualization, metagenomics

PhD in Biotechnology, Research Associate at Department of Biotechnology and Systems Biology, National Institute of Biology

Maja Zagorscak

Projects: pISA-tree, HYp - Spatiotemporal analysis of hypersensitive response to Potato virus Y in potato, INDIE - Biotechnological production of sustainable indole, _p_stRT, ADAPT - Accelerated Development of multiple-stress tolerAnt PoTato, tst, tst2

Institutions: National Institute of Biology, tst

https://orcid.org/0000-0002-1669-6482

Expertise: Molecular Biology, Statistics, Bioinformatics, Mathematical and statistical modeling, Programming, Data analysisMathematical modellingBioinformaticsSystems biology, Data Management, Data analysis, Visualization, Data Integration, Computational Biology

Tools: Bioinformatics, Computational and theoretical biology, Computational Systems Biology, Data Management, Databases, Dynamic modelling, Molecular Biology, Python, R, Systems Biology, Data Integration

Computational Biologist and Biostatistician at Department of Biotechnology and Systems Biology, National Institute of Biology (NIB)

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NIBSys

National Institute of Biology, Department of Biotechnology and Systems Biology projects

Projects: HYp - Spatiotemporal analysis of hypersensitive response to Potato virus Y in potato, pISA-tree, MOA - Multiomics analysis of potato response to Potato virus Y (PVY) infection, SUSPHIRE - Sustainable Bioproduction of Pheromones for Insect Pest Control in Agriculture, INDIE - Biotechnological production of sustainable indole, _p_stRT, ADAPT - Accelerated Development of multiple-stress tolerAnt PoTato, _p_RNAinVAL, tst, tst2, Playground

Web page: http://www.nib.si/eng/index.php/departments/department-of-biotechnology-and-systems-biology

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_p_stRT

project: _p_stRT Short Name: stRT Title: Solanum tuberosum Reference Transcriptomes Description: Cultivar-specific transcriptome and pan-transcriptome reconstruction of tetraploid potato pISA projects path: ../pISA-Projects Local pISA-tree organisation: National Institute of Biology pISA project creation date: 2019-10-22 pISA project creator: Maja Zagorscak, Ziva Ramsak, Marko Petek Project funding code: This project was supported by the Slovenian Research Agency (grants P4-0165, J4-4165, J4-7636, ...

Programme: NIBSys

Public web page: http://www.nib.si/eng/index.php/systems-biology

Start date: 1st Dec 2017

End date: 1st Jan 2020

Organisms: Potato virus Y, Solanum tuberosum

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_I_STRT

_p_stRT

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Maja Zagorscak

Kristina Gruden

Marko Petek

Ziva Ramsak

Investigation: _I_STRT Short Name: STRT Title: Cultivar-specific transcriptome and pan-transcriptome reconstruction of tetraploid potato Description: Cultivar-specific transcriptome and pan-transcriptome reconstruction of tetraploid potato Phenodata: ./phenodata_20191022.txt pISA Investigation creation date: 2019-10-22 pISA Investigation creator: Maja Zagorscak, Ziva Ramsak, Marko Petek Principal investigator: Kristina Gruden License: MIT Sharing permission: Public Upload to FAIRDOMHub: Yes

RELATED ...

Submitter: Maja Zagorscak

Studies: SupplementaryInformation, _S_01_sequences, _S_02_denovo, _S_03_stCuSTr, _S_04_stPanTr

Assays: Supplementary Information, _A_01_GC_content-count, _A_01_evigene, _A_02.1_BUSCO, _A_02.2_assembly-contribution-count, _A_02.3_InterProScan, _A_02.4_STAR, _A_02.5_STARlong_matchAnnot, _A_02.6_TransRate, _A_02.7_VecScreen, _A_02.8_DIAMOND, _A_02_cdhit_3cvs-GFFmerged, _A_03.1_filtering, _A_03.2_components, _A_03_components_3cvs-GFFmerged, _A_04_BUSCO_3cvs-GFFmerged, _A_04_TransRate, _A_05_BUSCO, _A_05_MSA_3cvs-GFFmerged, _A_06_tr_rep-transrate, _A_07_Desiree-mapping, _A_08_centrifuge_3cvs-GFFmerged, _A_09_annotation-GFFmerged

Snapshots: No snapshots

Created: 24th Oct 2019 at 14:36, Last updated: 26th Nov 2019 at 09:37

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_S_03_stCuSTr

_p_stRT

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Maja Zagorscak

Ziva Ramsak

Marko Petek

Study: _S_03_stCuSTr Short Name: 03_stCuSTr Title: Solanum tuberosum Cultivar-Specific Transcriptomes Description: Solanum tuberosum Cultivar-Specific Transcriptomes Raw Data: ../_S_01_sequences/, ../_S_02_denovo/ pISA Study creation date: 2019-10-22 pISA Study creator: Maja Zagorscak, Marko Petek, Ziva Ramsak Principal investigator: Kristina Gruden License: MIT Sharing permission: Public Upload to FAIRDOMHub: Yes

RELATED FILES: /reports/README.MD https://fairdomhub.org/documents/346 /_STUDY_METADATA.TXT ...

Submitter: Maja Zagorscak

Investigation: _I_STRT

Assays: _A_01_evigene, _A_02.1_BUSCO, _A_02.2_assembly-contribution-count, _A_02.3_InterProScan, _A_02.4_STAR, _A_02.5_STARlong_matchAnnot, _A_02.6_TransRate, _A_02.7_VecScreen, _A_02.8_DIAMOND, _A_03.1_filtering, _A_03.2_components, _A_04_TransRate, _A_05_BUSCO

Snapshots: No snapshots

Created: 24th Oct 2019 at 14:40, Last updated: 26th Nov 2019 at 09:36

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/reports/summaryCounts&SJ.xlsx

_p_stRT

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Maja Zagorscak

coverage and uniquely mapped spliced reads summary

Creator: Maja Zagorscak

Submitter: Maja Zagorscak

DOI: 10.15490/fairdomhub.1.datafile.3760.1

Relationship type: Not specified

Investigations: _I_STRT

Studies: _S_03_stCuSTr

Assays: _A_02.4_STAR

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Created: 2nd Jun 2020 at 13:42

/output/samtoolsCoverage.zip

_p_stRT

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Maja Zagorscak

samtools coverage https://www.htslib.org/doc/samtools-coverage.html

Creator: Maja Zagorscak

Submitter: Maja Zagorscak

Relationship type: Not specified

Investigations: _I_STRT

Studies: _S_03_stCuSTr

Assays: _A_02.4_STAR

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Created: 2nd Jun 2020 at 13:29

/input/path_to_files.txt

_p_stRT

All creators

Maja Zagorscak

No description specified

Creator: Maja Zagorscak

Submitter: Maja Zagorscak

Relationship type: Not specified

Investigations: _I_STRT

Studies: _S_03_stCuSTr

Assays: _A_02.4_STAR

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Created: 16th Nov 2019 at 11:59, Last updated: 25th Nov 2019 at 23:33

/output/output_STAR_logs-n-SJ.tar.gz

_p_stRT

All creators

Maja Zagorscak

No description specified

Creator: Maja Zagorscak

Submitter: Maja Zagorscak

Relationship type: Not specified

Investigations: _I_STRT

Studies: _S_03_stCuSTr

Assays: _A_02.4_STAR

Download

Created: 16th Nov 2019 at 12:00, Last updated: 25th Nov 2019 at 23:33

/scripts/STAR_commands.txt

_p_stRT

All creators

Maja Zagorscak

No description specified

Creator: Maja Zagorscak

Submitter: Maja Zagorscak

Relationship type: Not specified

Investigations: _I_STRT

Studies: _S_03_stCuSTr

Assays: _A_02.4_STAR

Download

Created: 16th Nov 2019 at 12:01, Last updated: 25th Nov 2019 at 23:33

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Cultivar-specific transcriptome and pan-transcriptome reconstruction of tetraploid potato

_p_stRT

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Marko Petek

Maja Zagorscak

Ziva Ramsak

Sheri Sanders

Anna Coll Rius

Kristina Gruden

Abstract (Expand)

Background: Although the reference genome of Solanum tuberosum group Phureja double-monoploid (DM) clone is available, knowledge on the genetic diversity of the highly heterozygous tetraploid group … Tuberosum, representing most cultivated varieties, remains largely unexplored. This lack of knowledge hinders further progress in potato research and its subsequent applications in breeding. Results: For the DM genome assembly, two only partially-overlapping gene models exist differing in a unique set of genes and intron/exon structure predictions. First step was to merge and manually curate the merged gene model, creating a union of genes in Phureja scaffold. We next compiled available RNA-Seq datasets (cca. 1.5 billion reads) for three tetraploid potato genotypes (cultivar Désirée, cultivar Rywal, and breeding clone PW363) with diverse breeding pedigrees. Short-read transcriptomes were assembled using CLC, Trinity, Velvet, and rnaSPAdes de novo assemblers using different settings to test for optimal outcome. In addition, for cultivar Rywal, PacBio Iso-Seq full-length transcriptome sequencing was also performed. Revised EvidentialGene redundancy-reducing pipeline was employed to produce accurate and complete cultivar-specific transcriptomes from assemblers output, as well as to attain the pan-transcriptome. Due to being the most diverse dataset in terms of tissues (stem, seedlings and roots) and experimental conditions, cv. Désirée was the most complete transcriptome (95.8% BUSCO completeness). For cv. Rywal and breeding clone PW363 data were available for leaf samples only and the resulting transcriptomes were less complete than cv. Désirée (89.8% and 89.3% BUSCO completeness, respectively). Cross comparison of these cultivar-specific transcriptomes and merged DM gene model suggests that the core potato transcriptome is comprised of 16,339 genes. The pan-transcriptome contains a total of 95,779 transcripts, of which 54,614 transcripts are not present in the Phureja genome. These represent the variants of the novel genes found in the potato pan-genome. Conclusions: Our analysis shows that the available gene model of double-monoploid potato from group Phureja is, to some degree, not complete. The generated transcriptomes and pan-transcriptome represent a valuable resource for potato gene variability exploration, high-throughput -omics analyses, and future breeding programmes.

Authors: Marko Petek, Maja Zagorščak, Živa Ramšak, Sheri Sanders, Elizabeth Tseng, Mohamed Zouine, Anna Coll, Kristina Gruden

Date Published: No date defined

Publication Type: Not specified

DOI: 10.1101/845818

Citation: Cultivar-specific transcriptome and pan-transcriptome reconstruction of tetraploid potato

Created: 19th Nov 2019 at 11:48, Last updated: 8th Dec 2022 at 17:26

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/_Assay_METADATA.TXT

_p_stRT