AT0010 4x 15:9-LL

Description

4 days of 15hrs light followed by 9hrs dark into constant light for 3 days

Purpose

changes to photoperiod

Comments

White fluffy infection on plate 4 - ELF3:LUC plate. Across whole plate

AT0009 4x 9:15-LL

Description

4 days 9hrs light, 15hrs dark followed by 3 days in constant light

Purpose

changes to photoperiod

Comments

missed first image, started 11.25 am

AT0061: 4x18:6-LL

Description

LUC imaging 18:6 LD to LL in RB light

Purpose

testing different photoperiod effect on entrainment and phase on release to LL

Comments

CCR2 in lhycca1 and lhy cca1 gi are contaminated.

AT0007 4x 03:21-LL

Description

4 days 3hrs light 21 hrs dark followed by 3 days constant

Purpose

changes to photoperiod

Comments

plate no changed comment in lab bk

AT0006 4x LD-3xLL

Description

4 days LD followed by 3 days constant light

Purpose

changes to photoperiod

Comments

AT0005 4x SD-3xLL

Description

alternate 4 days SD followed by 3 days constant light

Purpose

changes to photoperiod

Comments

AT0059: 4x15:09-LL

Description

LUC imaging 15:9 LD to LL in RB light

Purpose

testing different photoperiod effect on entrainment and phase on release to LL

Comments

LHy white & dead, white fungus over cca1 lhy gi CCR2 & cca1lhy gi CCR2 plate - does not affect CCR2

AT0057: 4x12:12-LL

Description

LUC imaging 12:12 LD to LL in RB light

Purpose

testing different photoperiod effect on entrainment and phase on release to LL

Comments

CCR2:LUC in cca1 lhy and in cca1lhygi had white fungal contamination all over plate ELF3:LUC had low germination

AT0055: 4x9:15-LL

Description

LUC imaging short days to LL in RB light

Purpose

testing short day entrainment and phase on release to LL

Comments

PRR9:LUC group dead, green colonies on 2x CCR2:LUC seedlings

AT0091: 4x12:12-LL

Description

LUC imaging 12L:12D into constant light

Purpose

testing variation of rhythmicity across camera field of view, in single seedlings with CCA1 or TOC1 marker

Comments

Original analysis by John O'Neill; Data curated by Andrew Millar.

AT0028: 4x 3:21-LL

Description

LUC imaging 3:21 blue+red into constant light, TOC1 data show anomalous dawn peak, so TOC1 data not used for phase analysis in Edwards et al. 2010

Purpose

testing circadian phase across photoperiods

Comments

AT0073: 4x12:12-DD

Description

LUC imaging 12L:12D into constant dark

Purpose

testing phase changes of clock components under different photoperiods

Comments

microwave seed sterilisation

AT0079: 4x12:12-DD

Description

LUC imaging 12L:12D into constant dark

Purpose

testing phase changes of clock components under different photoperiods

Comments

CCR2 plate (WT, lhy;cca1 and lhy;cca1;gi) infected all over by end of experiment

AT0077: 4x3:21-DD

Description

LUC imaging 3L:21D into constant dark

Purpose

testing phase changes of clock components under different photoperiods

Comments

AT0075: 4x18:6-DD

Description

LUC imaging 18L:6D into constant dark

Purpose

testing phase changes of clock components under different photoperiods

Comments

microwave seed sterilisation Pedro prepared by editing groups from previous template

AT0074: 4x15:9-DD

Description

LUC imaging 15L:9D into constant dark

Purpose

testing phase changes of clock components under different photoperiods

Comments

microwave seed sterilisation

Wenden_qPCR_clock_cFR, WT and elf4

Description

Literature data from: 'Light inputs shape the Arabidopsis circadian system' by: Benedicte Wenden.

Experiments performed on phyA and Col-0, 22 March to 26 Aug 2009, and on elf4, 8 March through November 2010. Published in Wenden et al. Plant Journal 2011. Curated ...

Wenden_qPCR_clock_cFR, WT and phyA

Description

Literature data from: 'Light inputs shape the Arabidopsis circadian system' by: Benedicte Wenden.

Experiments performed on phyA and Col-0, 22 March to 26 Aug 2009, and on elf4, 8 March through November 2010. Published in Wenden et al. Plant Journal 2011. Curated ...

Clock genes under Far Red light - 6

Description

Seedlings receive 3hrs of white light to germinate, then are entrained under 12/12 FR/D cycles for 3 days, sprayed, and imaged 24hrs after under 1 FR/D cycle, 3 days of continuous FR and 48h of DD

Purpose

Analysis of CCA1 and CCR2 in phyb, elf3-4, elf4-1 and ...

CCA1 and CCR2 markers in elf4 mutant under Far Red light

Description

Seedlings receive 3hrs of white light to germinate, then are entrained under 12/12 FR/D cycles for 3 days, sprayed, and imaged 24hrs after under 1 FR/D cycle, 3 days of continuous FR and 48h of DD

Purpose

Analysis of the CCA1 and CCR2 ...

Clock genes under Far Red light - 5

Description

Seedlings receive 3hrs of white light to germinate, then are entrained under 12/12 FR/D cycles for 3 days, sprayed, and imaged 24hrs after under 1 FR/D cycle, 3 days of continuous FR and 48h of DD

Purpose

Analysis of the main clock markers in elf4, elf3, gi ...

Clock genes under Far Red light - 3

Description

Seedlings receive 3hrs of white light to germinate, then are entrained under 12/12 FR/D cycles for 3 days, sprayed, and imaged 24hrs after under 1 FR/D cycle, 3 days of continuous FR and 48h of DD

Purpose

Analysis of the main clock markers under strict FR ...

Analysis of Control Strains Neurospora crassa

Description

Purpose

Compare different siblings of the parental/control strains in the global screening of transcription factors capable to affect the circadian oscilator

Comments

Carbon Source

Description

Purpose

Compare Control Strains in different Carbon Sources

Comments

Entrainment Control Analysis

Description

Purpose

Compare Period Calculations using entrainment and non entrainment strains for 72 hours

Comments

Controls

Description

Purpose

Global Search

Comments

080115H1

Description

Plants entrained in 12L 12D white light.

Experimental conditions (Red and Blue) 12 hours light (90umolm-2s-1): 12 hours shady dark (6 umolm-2s-1) x4, constant light x3. Dawn is at ZT0 and dusk (shady dark) is at ZT12.

Purpose

Comparison of Light:Shady night versus standard lights ON/OFF to dissect the role of photoreceptors and ...

080115H2

Description

Experimental conditions (Red and Blue) 12 hours light : 12 hours dark x4 followed by 3 days constant light. light is at 90umolm-2s-1 (red = 51 umolm-2s-1, blue = 32 umol-2s-1)

Purpose

Comparison of Light:Shady night versus standard lights ON/OFF to dissect the role of photoreceptors and photosynthesis in the circadian clock.

Comments

...

210814H1

Description

Plants entrained in 12L 12D white light.

Experimental conditions (Red and Blue) 12 hours light (90umolm-2s-1): 12 hours shady dark (6 umolm-2s-1) x7, Dawn is at ZT0 and dusk (shady dark) is at ZT12.

Purpose

Comparison of Light:Shady night versus standard lights ON/OFF to dissect the role of photoreceptors and photosynthesis in the ...

210814H2

Description

Experimental conditions (Red and Blue) 12 hours light : 12 hours dark x7 light is at 90umolm-2s-1 (red = 51 umolm-2s-1, blue = 32 umol-2s-1)

Purpose

Comparison of Light:Shady night versus standard lights ON/OFF to dissect the role of photoreceptors and photosynthesis in the circadian clock.

Comments

Partner experiment: 210814H1