Assays
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AT0007 4x 03:21-LL
| Property | Value |
|---|---|
| BioDare ID | 12730735093496 |
| Author | Adrian Thomson |
| Institution | University of Edinburgh |
| License | CC_BY |
Description
4 days 3hrs light 21 hrs dark followed by 3 days constant
Purpose
changes to photoperiod
Comments
plate no changed comment in lab bk
Submitter: Daniel Thedie
Assay type: Experimental Assay Type
Technology type: Technology Type
Investigation: Thomson Adrian
Study: Unpublished
AT0005 4x SD-3xLL
| Property | Value |
|---|---|
| BioDare ID | 12730734291594 |
| Author | Adrian Thomson |
| Institution | University of Edinburgh |
| License | CC_BY |
Description
alternate 4 days SD followed by 3 days constant light
Purpose
changes to photoperiod
Comments
Submitter: Daniel Thedie
Assay type: Experimental Assay Type
Technology type: Technology Type
Investigation: Thomson Adrian
Study: Unpublished
AT0006 4x LD-3xLL
| Property | Value |
|---|---|
| BioDare ID | 12730734647405 |
| Author | Adrian Thomson |
| Institution | University of Edinburgh |
| License | CC_BY |
Description
4 days LD followed by 3 days constant light
Purpose
changes to photoperiod
Comments
Submitter: Daniel Thedie
Assay type: Experimental Assay Type
Technology type: Technology Type
Investigation: Thomson Adrian
Study: Unpublished
AT0059: 4x15:09-LL
| Property | Value |
|---|---|
| BioDare ID | 12730733878982 |
| Author | Adrian Thomson |
| Institution | University of Edinburgh |
| License | CC_BY |
Description
LUC imaging 15:9 LD to LL in RB light
Purpose
testing different photoperiod effect on entrainment and phase on release to LL
Comments
LHy white & dead, white fungus over cca1 lhy gi CCR2 & cca1lhy gi CCR2 plate - does not affect CCR2
Submitter: Daniel Thedie
Assay type: Experimental Assay Type
Technology type: Technology Type
Investigation: Thomson Adrian
Study: Unpublished
AT0055: 4x9:15-LL
| Property | Value |
|---|---|
| BioDare ID | 12730725716347 |
| Author | Adrian Thomson |
| Institution | University of Edinburgh |
| License | CC_BY |
Description
LUC imaging short days to LL in RB light
Purpose
testing short day entrainment and phase on release to LL
Comments
PRR9:LUC group dead, green colonies on 2x CCR2:LUC seedlings
Submitter: Daniel Thedie
Assay type: Experimental Assay Type
Technology type: Technology Type
Investigation: Thomson Adrian
Study: Unpublished
AT0028: 4x 3:21-LL
| Property | Value |
|---|---|
| BioDare ID | 12730555174222 |
| Author | Adrian Thomson |
| Institution | University of Edinburgh |
| License | CC_BY |
Description
LUC imaging 3:21 blue+red into constant light, TOC1 data show anomalous dawn peak, so TOC1 data not used for phase analysis in Edwards et al. 2010
Purpose
testing circadian phase across photoperiods
Comments
Submitter: Daniel Thedie
Assay type: Experimental Assay Type
Technology type: Technology Type
Investigation: Thomson Adrian
Study: Unpublished
AT0073: 4x12:12-DD
| Property | Value |
|---|---|
| BioDare ID | 12730509509211 |
| Author | Adrian Thomson |
| Institution | University of Edinburgh |
| License | CC_BY |
Description
LUC imaging 12L:12D into constant dark
Purpose
testing phase changes of clock components under different photoperiods
Comments
microwave seed sterilisation
Submitter: Daniel Thedie
Assay type: Experimental Assay Type
Technology type: Technology Type
Investigation: Thomson Adrian
Study: Unpublished
AT0079: 4x12:12-DD
| Property | Value |
|---|---|
| BioDare ID | 12730498782920 |
| Author | Adrian Thomson |
| Institution | University of Edinburgh |
| License | CC_BY |
Description
LUC imaging 12L:12D into constant dark
Purpose
testing phase changes of clock components under different photoperiods
Comments
CCR2 plate (WT, lhy;cca1 and lhy;cca1;gi) infected all over by end of experiment
Submitter: Daniel Thedie
Assay type: Experimental Assay Type
Technology type: Technology Type
Investigation: Thomson Adrian
Study: Unpublished
AT0075: 4x18:6-DD
| Property | Value |
|---|---|
| BioDare ID | 12729849314623 |
| Author | Adrian Thomson |
| Institution | University of Edinburgh |
| License | CC_BY |
Description
LUC imaging 18L:6D into constant dark
Purpose
testing phase changes of clock components under different photoperiods
Comments
microwave seed sterilisation Pedro prepared by editing groups from previous template
Submitter: Daniel Thedie
Assay type: Experimental Assay Type
Technology type: Technology Type
Investigation: Thomson Adrian
Study: Unpublished
AT0077: 4x3:21-DD
| Property | Value |
|---|---|
| BioDare ID | 12729891771943 |
| Author | Adrian Thomson |
| Institution | University of Edinburgh |
| License | CC_BY |
Description
LUC imaging 3L:21D into constant dark
Purpose
testing phase changes of clock components under different photoperiods
Comments
Submitter: Daniel Thedie
Assay type: Experimental Assay Type
Technology type: Technology Type
Investigation: Thomson Adrian
Study: Unpublished
AT0074: 4x15:9-DD
| Property | Value |
|---|---|
| BioDare ID | 12729820597730 |
| Author | Adrian Thomson |
| Institution | University of Edinburgh |
| License | CC_BY |
Description
LUC imaging 15L:9D into constant dark
Purpose
testing phase changes of clock components under different photoperiods
Comments
microwave seed sterilisation
Submitter: Daniel Thedie
Assay type: Experimental Assay Type
Technology type: Technology Type
Investigation: Thomson Adrian
Study: Unpublished
Wenden_qPCR_clock_cFR, WT and elf4
| Property | Value |
|---|---|
| BioDare ID | 13395775064583 |
| Author | Benedicte Wenden |
| Institution | University of Edinburgh |
| License | CC_BY |
Description
Literature data from: 'Light inputs shape the Arabidopsis circadian system' by: Benedicte Wenden.
Experiments performed on phyA and Col-0, 22 March to 26 Aug 2009, and on elf4, 8 March through November 2010. Published in Wenden et al. Plant Journal 2011. Curated ...
Submitter: Daniel Thedie
Assay type: Experimental Assay Type
Technology type: Technology Type
Investigation: Wenden Benedicte
Study: Unpublished
Wenden_qPCR_clock_cFR, WT and phyA
| Property | Value |
|---|---|
| BioDare ID | 13395774721132 |
| Author | Benedicte Wenden |
| Institution | University of Edinburgh |
| License | CC_BY |
Description
Literature data from: 'Light inputs shape the Arabidopsis circadian system' by: Benedicte Wenden.
Experiments performed on phyA and Col-0, 22 March to 26 Aug 2009, and on elf4, 8 March through November 2010. Published in Wenden et al. Plant Journal 2011. Curated ...
Submitter: Daniel Thedie
Assay type: Experimental Assay Type
Technology type: Technology Type
Investigation: Wenden Benedicte
Study: Unpublished
CCA1 and CCR2 markers in elf4 mutant under Far Red light
| Property | Value |
|---|---|
| BioDare ID | 12725469475452 |
| Author | Benedicte Wenden |
| Institution | University of Edinburgh |
| License | CC_BY |
Description
Seedlings receive 3hrs of white light to germinate, then are entrained under 12/12 FR/D cycles for 3 days, sprayed, and imaged 24hrs after under 1 FR/D cycle, 3 days of continuous FR and 48h of DD
Purpose
Analysis of the CCA1 and CCR2 ...
Submitter: Daniel Thedie
Assay type: Experimental Assay Type
Technology type: Technology Type
Investigation: Wenden Benedicte
Study: Unpublished
Clock genes under Far Red light - 6
| Property | Value |
|---|---|
| BioDare ID | 12730680847492 |
| Author | Benedicte Wenden |
| Institution | University of Edinburgh |
| License | CC_BY |
Description
Seedlings receive 3hrs of white light to germinate, then are entrained under 12/12 FR/D cycles for 3 days, sprayed, and imaged 24hrs after under 1 FR/D cycle, 3 days of continuous FR and 48h of DD
Purpose
Analysis of CCA1 and CCR2 in phyb, elf3-4, elf4-1 and ...
Submitter: Daniel Thedie
Assay type: Experimental Assay Type
Technology type: Technology Type
Investigation: Wenden Benedicte
Study: Unpublished
Clock genes under Far Red light - 5
| Property | Value |
|---|---|
| BioDare ID | 12725464084711 |
| Author | Benedicte Wenden |
| Institution | University of Edinburgh |
| License | CC_BY |
Description
Seedlings receive 3hrs of white light to germinate, then are entrained under 12/12 FR/D cycles for 3 days, sprayed, and imaged 24hrs after under 1 FR/D cycle, 3 days of continuous FR and 48h of DD
Purpose
Analysis of the main clock markers in elf4, elf3, gi ...
Submitter: Daniel Thedie
Assay type: Experimental Assay Type
Technology type: Technology Type
Investigation: Wenden Benedicte
Study: Unpublished
Clock genes under Far Red light - 3
| Property | Value |
|---|---|
| BioDare ID | 12723746109930 |
| Author | Benedicte Wenden |
| Institution | University of Edinburgh |
| License | CC_BY |
Description
Seedlings receive 3hrs of white light to germinate, then are entrained under 12/12 FR/D cycles for 3 days, sprayed, and imaged 24hrs after under 1 FR/D cycle, 3 days of continuous FR and 48h of DD
Purpose
Analysis of the main clock markers under strict FR ...
Submitter: Daniel Thedie
Assay type: Experimental Assay Type
Technology type: Technology Type
Investigation: Wenden Benedicte
Study: Unpublished
080115H1
| Property | Value |
|---|---|
| BioDare ID | 2629 |
| Author | Yvette Wilson |
| Institution | |
| License |
Description
Plants entrained in 12L 12D white light.
Experimental conditions (Red and Blue) 12 hours light (90umolm-2s-1): 12 hours shady dark (6 umolm-2s-1) x4, constant light x3. Dawn is at ZT0 and dusk (shady dark) is at ZT12.
Purpose
Comparison of Light:Shady night versus standard lights ON/OFF to dissect the role of photoreceptors and ...
Submitter: Daniel Thedie
Assay type: Experimental Assay Type
Technology type: Technology Type
Investigation: Millar Andrew
Study: TiMet shady nights
080115H2
| Property | Value |
|---|---|
| BioDare ID | 2630 |
| Author | Yvette Wilson |
| Institution | |
| License |
Description
Experimental conditions (Red and Blue) 12 hours light : 12 hours dark x4 followed by 3 days constant light. light is at 90umolm-2s-1 (red = 51 umolm-2s-1, blue = 32 umol-2s-1)
Purpose
Comparison of Light:Shady night versus standard lights ON/OFF to dissect the role of photoreceptors and photosynthesis in the circadian clock.
Comments
...
Submitter: Daniel Thedie
Assay type: Experimental Assay Type
Technology type: Technology Type
Investigation: Millar Andrew
Study: TiMet shady nights
210814H2
| Property | Value |
|---|---|
| BioDare ID | 1926 |
| Author | Yvette Wilson |
| Institution | |
| License |
Description
Experimental conditions (Red and Blue) 12 hours light : 12 hours dark x7 light is at 90umolm-2s-1 (red = 51 umolm-2s-1, blue = 32 umol-2s-1)
Purpose
Comparison of Light:Shady night versus standard lights ON/OFF to dissect the role of photoreceptors and photosynthesis in the circadian clock.
Comments
Partner experiment: 210814H1
Submitter: Daniel Thedie
Assay type: Experimental Assay Type
Technology type: Technology Type
Investigation: Millar Andrew
Study: TiMet shady nights
210814H1
| Property | Value |
|---|---|
| BioDare ID | 1925 |
| Author | Yvette Wilson |
| Institution | |
| License |
Description
Plants entrained in 12L 12D white light.
Experimental conditions (Red and Blue) 12 hours light (90umolm-2s-1): 12 hours shady dark (6 umolm-2s-1) x7, Dawn is at ZT0 and dusk (shady dark) is at ZT12.
Purpose
Comparison of Light:Shady night versus standard lights ON/OFF to dissect the role of photoreceptors and photosynthesis in the ...
Submitter: Daniel Thedie
Assay type: Experimental Assay Type
Technology type: Technology Type
Investigation: Millar Andrew
Study: TiMet shady nights
190614H1
| Property | Value |
|---|---|
| BioDare ID | 1642 |
| Author | Yvette Wilson |
| Institution | |
| License |
Description
Plants entrained in 12L 12D white light.
Experimental conditions (Red and Blue) 12 hours light (90umolm-2s-1): 12 hours shady dark (6 umolm-2s-1) x4, constant light x3. Dawn is at ZT0 and dusk (shady dark) is at ZT12.
Purpose
Comparison of Light:Shady night versus standard lights ON/OFF to dissect the role of photoreceptors and ...
Submitter: Daniel Thedie
Assay type: Experimental Assay Type
Technology type: Technology Type
Investigation: Millar Andrew
Study: TiMet shady nights
190614H2
| Property | Value |
|---|---|
| BioDare ID | 1643 |
| Author | Yvette Wilson |
| Institution | |
| License |
Description
Experimental conditions (Red and Blue) 12 hours light : 12 hours dark x4 followed by 3 days constant light. light is at 90umolm-2s-1 (red = 51 umolm-2s-1, blue = 32 umol-2s-1)
Purpose
Comparison of Light:Shady night versus standard lights ON/OFF to dissect the role of photoreceptors and photosynthesis in the circadian clock.
Comments
...
Submitter: Daniel Thedie
Assay type: Experimental Assay Type
Technology type: Technology Type
Investigation: Millar Andrew
Study: TiMet shady nights
270313H1: 21L 7Dx4 LLx3 (T-28)
| Property | Value |
|---|---|
| BioDare ID | 13649842953637 |
| Author | Wei Ven Tee |
| Institution | University of Edinburgh |
| License | CC_BY |
Description
Plants entrained in 21L 7D Experimental conditions: 21L7Dx4 LLx3
Purpose
To test whether cca1/lhy double mutant can adjust to change in photoperiod in T-cycle
Comments
5mins of light at the end of daytime during the first three imaging days appear to be absent, ...
Submitter: Daniel Thedie
Assay type: Experimental Assay Type
Technology type: Technology Type
Investigation: Millar Andrew
Study: Millar et al. (2015)
200313H2: 7L 21Dx4 LLx3 (T-28)
| Property | Value |
|---|---|
| BioDare ID | 13643892291642 |
| Author | Wei Ven Tee |
| Institution | University of Edinburgh |
| License | CC_BY |
Description
Plants entrained in 7L 21D Experimental conditions: 7L21Dx4 LLx3
Purpose
To test whether cca1/lhy double mutant can adjust to change in photoperiod in T-cycle
Comments
5mins of light at the end of daytime on imaging day 2 and day 3 appeared to be absent, using the ...
Submitter: Daniel Thedie
Assay type: Experimental Assay Type
Technology type: Technology Type
Investigation: Millar Andrew
Study: Millar et al. (2015)
290313H2: 15L 5Dx4 LLx3
| Property | Value |
|---|---|
| BioDare ID | 13649886952409 |
| Author | Wei Ven Tee |
| Institution | University of Edinburgh |
| License | CC_BY |
Description
Plants entrained in 15L 5D Experimental conditions: 15L 5Dx4 LLx3
Purpose
To test whether cca1/lhy double mutant can adjust to change in photoperiod in T-cycle
Comments
5mins of light at the end of daytime during the first three imaging days appear to be absent, using the ...
Submitter: Daniel Thedie
Assay type: Experimental Assay Type
Technology type: Technology Type
Investigation: Millar Andrew
Study: Millar et al. (2015)
270313H3: 12L-12Dx4 LLx3
| Property | Value |
|---|---|
| BioDare ID | 13650039169340 |
| Author | Jamie Carrington |
| Institution | University of Edinburgh |
| License | CC_BY |
Description
Plants entrained in 12L 12D white light Experimental conditions: 12L-12Dx4 LLx3 ('white')
Purpose
'White' (red+blue) light control experiment for the phytochrome and cryptochrome experiments
Comments
Submitter: Daniel Thedie
Assay type: Experimental Assay Type
Technology type: Technology Type
Investigation: Millar Andrew
Study: Millar et al. (2015)
200313H3: 12L-12Dx4 LLx3
| Property | Value |
|---|---|
| BioDare ID | 13644021710933 |
| Author | Jamie Carrington |
| Institution | University of Edinburgh |
| License | CC_BY |
Description
Plants entrained in 12L 12D white light Experimental conditions: 12L-12Dx4 LLx3 ('white')
Purpose
'White' (red+blue) light control experiment for the phytochrome and cryptochrome experiments
Comments
Submitter: Daniel Thedie
Assay type: Experimental Assay Type
Technology type: Technology Type
Investigation: Millar Andrew
Study: Millar et al. (2015)
060213H3: 12B-12Dx4 BBx3
| Property | Value |
|---|---|
| BioDare ID | 13615365050841 |
| Author | Jamie Carrington |
| Institution | University of Edinburgh |
| License | CC_BY |
Description
Plants entrained in 12L 12D white light Experimental conditions: 12L-12Dx4 LLx3 (Blue)
Purpose
Test how the cryptochromes interact with the circadian clock (WT, lhy/cca1 double mutant and lhy/cca1/gi triple mutant)
Comments
Imaging script started at ZT+7mins, therefore ...
Submitter: Daniel Thedie
Assay type: Experimental Assay Type
Technology type: Technology Type
Investigation: Millar Andrew
Study: Millar et al. (2015)
030413H3: 12B-12Dx4 BB3
| Property | Value |
|---|---|
| BioDare ID | 13661271159675 |
| Author | Jamie Carrington |
| Institution | University of Edinburgh |
| License | CC_BY |
Description
Plants entrained in 12L 12D white light Experimental conditions: 12L-12Dx4 LLx3 (Blue)
Purpose
Test how the cryptochromes interact with the circadian clock (WT, lhy/cca1 double mutant and lhy/cca1/gi triple mutant)
Comments
Submitter: Daniel Thedie
Assay type: Experimental Assay Type
Technology type: Technology Type
Investigation: Millar Andrew
Study: Millar et al. (2015)