Assays

What is an Assay?
1653 Assays visible to you, out of a total of 2679

AT0007 4x 03:21-LL

Property Value
BioDare ID 12730735093496
Author Adrian Thomson
Institution University of Edinburgh
License CC_BY

Description

4 days 3hrs light 21 hrs dark followed by 3 days constant

Purpose

changes to photoperiod

Comments

plate no changed comment in lab bk

AT0005 4x SD-3xLL

Property Value
BioDare ID 12730734291594
Author Adrian Thomson
Institution University of Edinburgh
License CC_BY

Description

alternate 4 days SD followed by 3 days constant light

Purpose

changes to photoperiod

Comments

AT0006 4x LD-3xLL

Property Value
BioDare ID 12730734647405
Author Adrian Thomson
Institution University of Edinburgh
License CC_BY

Description

4 days LD followed by 3 days constant light

Purpose

changes to photoperiod

Comments

AT0059: 4x15:09-LL

Property Value
BioDare ID 12730733878982
Author Adrian Thomson
Institution University of Edinburgh
License CC_BY

Description

LUC imaging 15:9 LD to LL in RB light

Purpose

testing different photoperiod effect on entrainment and phase on release to LL

Comments

LHy white & dead, white fungus over cca1 lhy gi CCR2 & cca1lhy gi CCR2 plate - does not affect CCR2

AT0055: 4x9:15-LL

Property Value
BioDare ID 12730725716347
Author Adrian Thomson
Institution University of Edinburgh
License CC_BY

Description

LUC imaging short days to LL in RB light

Purpose

testing short day entrainment and phase on release to LL

Comments

PRR9:LUC group dead, green colonies on 2x CCR2:LUC seedlings

AT0028: 4x 3:21-LL

Property Value
BioDare ID 12730555174222
Author Adrian Thomson
Institution University of Edinburgh
License CC_BY

Description

LUC imaging 3:21 blue+red into constant light, TOC1 data show anomalous dawn peak, so TOC1 data not used for phase analysis in Edwards et al. 2010

Purpose

testing circadian phase across photoperiods

Comments

AT0073: 4x12:12-DD

Property Value
BioDare ID 12730509509211
Author Adrian Thomson
Institution University of Edinburgh
License CC_BY

Description

LUC imaging 12L:12D into constant dark

Purpose

testing phase changes of clock components under different photoperiods

Comments

microwave seed sterilisation

AT0079: 4x12:12-DD

Property Value
BioDare ID 12730498782920
Author Adrian Thomson
Institution University of Edinburgh
License CC_BY

Description

LUC imaging 12L:12D into constant dark

Purpose

testing phase changes of clock components under different photoperiods

Comments

CCR2 plate (WT, lhy;cca1 and lhy;cca1;gi) infected all over by end of experiment

AT0075: 4x18:6-DD

Property Value
BioDare ID 12729849314623
Author Adrian Thomson
Institution University of Edinburgh
License CC_BY

Description

LUC imaging 18L:6D into constant dark

Purpose

testing phase changes of clock components under different photoperiods

Comments

microwave seed sterilisation Pedro prepared by editing groups from previous template

AT0077: 4x3:21-DD

Property Value
BioDare ID 12729891771943
Author Adrian Thomson
Institution University of Edinburgh
License CC_BY

Description

LUC imaging 3L:21D into constant dark

Purpose

testing phase changes of clock components under different photoperiods

Comments

AT0074: 4x15:9-DD

Property Value
BioDare ID 12729820597730
Author Adrian Thomson
Institution University of Edinburgh
License CC_BY

Description

LUC imaging 15L:9D into constant dark

Purpose

testing phase changes of clock components under different photoperiods

Comments

microwave seed sterilisation

Wenden_qPCR_clock_cFR, WT and elf4

Property Value
BioDare ID 13395775064583
Author Benedicte Wenden
Institution University of Edinburgh
License CC_BY

Description

Literature data from: 'Light inputs shape the Arabidopsis circadian system' by: Benedicte Wenden.

Experiments performed on phyA and Col-0, 22 March to 26 Aug 2009, and on elf4, 8 March through November 2010. Published in Wenden et al. Plant Journal 2011. Curated ...

Wenden_qPCR_clock_cFR, WT and phyA

Property Value
BioDare ID 13395774721132
Author Benedicte Wenden
Institution University of Edinburgh
License CC_BY

Description

Literature data from: 'Light inputs shape the Arabidopsis circadian system' by: Benedicte Wenden.

Experiments performed on phyA and Col-0, 22 March to 26 Aug 2009, and on elf4, 8 March through November 2010. Published in Wenden et al. Plant Journal 2011. Curated ...

CCA1 and CCR2 markers in elf4 mutant under Far Red light

Property Value
BioDare ID 12725469475452
Author Benedicte Wenden
Institution University of Edinburgh
License CC_BY

Description

Seedlings receive 3hrs of white light to germinate, then are entrained under 12/12 FR/D cycles for 3 days, sprayed, and imaged 24hrs after under 1 FR/D cycle, 3 days of continuous FR and 48h of DD

Purpose

Analysis of the CCA1 and CCR2 ...

Submitter: Daniel Thedie

Assay type: Experimental Assay Type

Technology type: Technology Type

Investigation: Wenden Benedicte

Study: Unpublished

Clock genes under Far Red light - 6

Property Value
BioDare ID 12730680847492
Author Benedicte Wenden
Institution University of Edinburgh
License CC_BY

Description

Seedlings receive 3hrs of white light to germinate, then are entrained under 12/12 FR/D cycles for 3 days, sprayed, and imaged 24hrs after under 1 FR/D cycle, 3 days of continuous FR and 48h of DD

Purpose

Analysis of CCA1 and CCR2 in phyb, elf3-4, elf4-1 and ...

Clock genes under Far Red light - 5

Property Value
BioDare ID 12725464084711
Author Benedicte Wenden
Institution University of Edinburgh
License CC_BY

Description

Seedlings receive 3hrs of white light to germinate, then are entrained under 12/12 FR/D cycles for 3 days, sprayed, and imaged 24hrs after under 1 FR/D cycle, 3 days of continuous FR and 48h of DD

Purpose

Analysis of the main clock markers in elf4, elf3, gi ...

Clock genes under Far Red light - 3

Property Value
BioDare ID 12723746109930
Author Benedicte Wenden
Institution University of Edinburgh
License CC_BY

Description

Seedlings receive 3hrs of white light to germinate, then are entrained under 12/12 FR/D cycles for 3 days, sprayed, and imaged 24hrs after under 1 FR/D cycle, 3 days of continuous FR and 48h of DD

Purpose

Analysis of the main clock markers under strict FR ...

Submitter: Daniel Thedie

Assay type: Experimental Assay Type

Technology type: Technology Type

Investigation: Wenden Benedicte

Study: Unpublished

080115H1

Property Value
BioDare ID 2629
Author Yvette Wilson
Institution
License

Description

Plants entrained in 12L 12D white light.

Experimental conditions (Red and Blue) 12 hours light (90umolm-2s-1): 12 hours shady dark (6 umolm-2s-1) x4, constant light x3. Dawn is at ZT0 and dusk (shady dark) is at ZT12.

Purpose

Comparison of Light:Shady night versus standard lights ON/OFF to dissect the role of photoreceptors and ...

080115H2

Property Value
BioDare ID 2630
Author Yvette Wilson
Institution
License

Description

Experimental conditions (Red and Blue) 12 hours light : 12 hours dark x4 followed by 3 days constant light. light is at 90umolm-2s-1 (red = 51 umolm-2s-1, blue = 32 umol-2s-1)

Purpose

Comparison of Light:Shady night versus standard lights ON/OFF to dissect the role of photoreceptors and photosynthesis in the circadian clock.

Comments

...

Submitter: Daniel Thedie

Assay type: Experimental Assay Type

Technology type: Technology Type

Investigation: Millar Andrew

Study: TiMet shady nights

210814H2

Property Value
BioDare ID 1926
Author Yvette Wilson
Institution
License

Description

Experimental conditions (Red and Blue) 12 hours light : 12 hours dark x7 light is at 90umolm-2s-1 (red = 51 umolm-2s-1, blue = 32 umol-2s-1)

Purpose

Comparison of Light:Shady night versus standard lights ON/OFF to dissect the role of photoreceptors and photosynthesis in the circadian clock.

Comments

Partner experiment: 210814H1

Submitter: Daniel Thedie

Assay type: Experimental Assay Type

Technology type: Technology Type

Investigation: Millar Andrew

Study: TiMet shady nights

210814H1

Property Value
BioDare ID 1925
Author Yvette Wilson
Institution
License

Description

Plants entrained in 12L 12D white light.

Experimental conditions (Red and Blue) 12 hours light (90umolm-2s-1): 12 hours shady dark (6 umolm-2s-1) x7, Dawn is at ZT0 and dusk (shady dark) is at ZT12.

Purpose

Comparison of Light:Shady night versus standard lights ON/OFF to dissect the role of photoreceptors and photosynthesis in the ...

190614H1

Property Value
BioDare ID 1642
Author Yvette Wilson
Institution
License

Description

Plants entrained in 12L 12D white light.

Experimental conditions (Red and Blue) 12 hours light (90umolm-2s-1): 12 hours shady dark (6 umolm-2s-1) x4, constant light x3. Dawn is at ZT0 and dusk (shady dark) is at ZT12.

Purpose

Comparison of Light:Shady night versus standard lights ON/OFF to dissect the role of photoreceptors and ...

190614H2

Property Value
BioDare ID 1643
Author Yvette Wilson
Institution
License

Description

Experimental conditions (Red and Blue) 12 hours light : 12 hours dark x4 followed by 3 days constant light. light is at 90umolm-2s-1 (red = 51 umolm-2s-1, blue = 32 umol-2s-1)

Purpose

Comparison of Light:Shady night versus standard lights ON/OFF to dissect the role of photoreceptors and photosynthesis in the circadian clock.

Comments

...

Submitter: Daniel Thedie

Assay type: Experimental Assay Type

Technology type: Technology Type

Investigation: Millar Andrew

Study: TiMet shady nights

270313H1: 21L 7Dx4 LLx3 (T-28)

Property Value
BioDare ID 13649842953637
Author Wei Ven Tee
Institution University of Edinburgh
License CC_BY

Description

Plants entrained in 21L 7D Experimental conditions: 21L7Dx4 LLx3

Purpose

To test whether cca1/lhy double mutant can adjust to change in photoperiod in T-cycle

Comments

5mins of light at the end of daytime during the first three imaging days appear to be absent, ...

200313H2: 7L 21Dx4 LLx3 (T-28)

Property Value
BioDare ID 13643892291642
Author Wei Ven Tee
Institution University of Edinburgh
License CC_BY

Description

Plants entrained in 7L 21D Experimental conditions: 7L21Dx4 LLx3

Purpose

To test whether cca1/lhy double mutant can adjust to change in photoperiod in T-cycle

Comments

5mins of light at the end of daytime on imaging day 2 and day 3 appeared to be absent, using the ...

290313H2: 15L 5Dx4 LLx3

Property Value
BioDare ID 13649886952409
Author Wei Ven Tee
Institution University of Edinburgh
License CC_BY

Description

Plants entrained in 15L 5D Experimental conditions: 15L 5Dx4 LLx3

Purpose

To test whether cca1/lhy double mutant can adjust to change in photoperiod in T-cycle

Comments

5mins of light at the end of daytime during the first three imaging days appear to be absent, using the ...

270313H3: 12L-12Dx4 LLx3

Property Value
BioDare ID 13650039169340
Author Jamie Carrington
Institution University of Edinburgh
License CC_BY

Description

Plants entrained in 12L 12D white light Experimental conditions: 12L-12Dx4 LLx3 ('white')

Purpose

'White' (red+blue) light control experiment for the phytochrome and cryptochrome experiments

Comments

200313H3: 12L-12Dx4 LLx3

Property Value
BioDare ID 13644021710933
Author Jamie Carrington
Institution University of Edinburgh
License CC_BY

Description

Plants entrained in 12L 12D white light Experimental conditions: 12L-12Dx4 LLx3 ('white')

Purpose

'White' (red+blue) light control experiment for the phytochrome and cryptochrome experiments

Comments

060213H3: 12B-12Dx4 BBx3

Property Value
BioDare ID 13615365050841
Author Jamie Carrington
Institution University of Edinburgh
License CC_BY

Description

Plants entrained in 12L 12D white light Experimental conditions: 12L-12Dx4 LLx3 (Blue)

Purpose

Test how the cryptochromes interact with the circadian clock (WT, lhy/cca1 double mutant and lhy/cca1/gi triple mutant)

Comments

Imaging script started at ZT+7mins, therefore ...

030413H3: 12B-12Dx4 BB3

Property Value
BioDare ID 13661271159675
Author Jamie Carrington
Institution University of Edinburgh
License CC_BY

Description

Plants entrained in 12L 12D white light Experimental conditions: 12L-12Dx4 LLx3 (Blue)

Purpose

Test how the cryptochromes interact with the circadian clock (WT, lhy/cca1 double mutant and lhy/cca1/gi triple mutant)

Comments

Powered by
(v.1.18.2)