_I_T23_SxF
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Short Name: T23_SxF Title: Moth pheromone production in filamentous fungi SxF Description: Demonstration of moth pheromone production in filamentous fungi by solvent extractions of liquid cultures and mycelial biomass and GC-MS/MS analysis. Constitutive and inducible expression of moth pheromones in filamentous fungi Penicillium species will be developed using a dCas9-based copper inducible system. An initial proof of concept will also be performed in P. digitatum using constitutive promoters. Phenodata: ./phenodata_21Sep21.txt pISA Investigation creation date: 21-Sep-21 pISA Investigation creator: Sandra Vacas
Investigation position:
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Created: 12th Apr 2022 at 15:59
Last updated: 7th Nov 2024 at 10:04
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https://orcid.org/0000-0003-3644-7827PhD in Biotechnology, Research Associate at Department of Biotechnology and Systems Biology, National Institute of Biology
National Institute of Biology, Department of Biotechnology and Systems Biology projects
Projects: HYp - Spatiotemporal analysis of hypersensitive response to Potato virus Y in potato, pISA-tree, MOA - Multiomics analysis of potato response to Potato virus Y (PVY) infection, SUSPHIRE - Sustainable Bioproduction of Pheromones for Insect Pest Control in Agriculture, INDIE - Biotechnological production of sustainable indole, _p_stRT, ADAPT - Accelerated Development of multiple-stress tolerAnt PoTato, _p_RNAinVAL, tst, tst2, Playground
Web page: http://www.nib.si/eng/index.php/departments/department-of-biotechnology-and-systems-biology
SUSPHIRE aims to provide a sustainable, low-cost biomanufacturing platform for the commercial production of insect pheromones.
Programme: NIBSys
Public web page: http://susphire.info/susphireproject/
Start date: 1st Apr 2018
End date: 30th Sep 2021
Short Name: P1_ConstExp_Pdigitatum Title: Constitutive expression of moth pheromone pathway in Penicillium digitatum. Description: For this Proof of Concept, the three enzymes of the moth pheromones route were assembled in TUs using fungal promoters and terminators, and transformed into Penicillium digitatum. Pheromone production of the selected transformants will be assessed via GC-MS. Raw Data: pISA Study creation date: 2020-18-04 pISA Study creator: ElenaMG Principal investigator: Diego Orzaez ...
Submitter: Marko Petek
Investigation: _I_T23_SxF
Assays: _A_qRTPCRMothPheromonesEnzymes-Q-RT-PCR, _S_P1_ConstExp_Pdigitatum-files
Short Name: P5_SxFliquids Title: Pheromone detection in liquid extracts of SxF Description: Pheromone detection in liquid extracts of modified filamentous fungi (SxF) by liquid-liquid solvent extraction and GC-MS/MS analysis Raw Data: pISA Study creation date: 21-Sep-22 pISA Study creator: Sandra Vacas Principal investigator: * License: CC BY 4.0 Sharing permission: Private Upload to FAIRDOMHub: Yes
Submitter: Marko Petek
Investigation: _I_T23_SxF
Assays: _A_FS-GC-MS, _A_SRM-GC-MS, _S_P5_SxFliquids-files
Short Name: P5_SxFmycelia Title: Pheromone detection in SxF mycelial biomass Description: Pheromone detection in the mycelial biomass of modified filamentous fungi by solid-liquid solvent extraction and GC-MS/MS analysis Raw Data: pISA Study creation date: 21-Sep-22 pISA Study creator: Sandra Vacas Principal investigator: * License: CC BY 4.0 Sharing permission: Private Upload to FAIRDOMHub: Yes
Submitter: Marko Petek
Investigation: _I_T23_SxF
Assays: _A_FSM-GC-MS, _A_SRMM-GC-MS, _S_P5_SxFmycelia-files
Investigation files _I_T23_SxF
Files connected to investigation: _I_T23_SxF
Submitter: Marko Petek
Assay type: Experimental Assay Type
Technology type: Technology Type
Investigation: _I_T23_SxF
Study: Investigation files
Files connected to study: _S_P1_ConstExp_Pdigitatum
Submitter: Marko Petek
Assay type: Experimental Assay Type
Technology type: Technology Type
Investigation: _I_T23_SxF
Study: _S_P1_ConstExp_Pdigitatum
Files connected to study: _S_P5_SxFliquids
Submitter: Marko Petek
Assay type: Experimental Assay Type
Technology type: Technology Type
Investigation: _I_T23_SxF
Study: _S_P5_SxFliquids
Files connected to study: _S_P5_SxFmycelia
Submitter: Marko Petek
Assay type: Experimental Assay Type
Technology type: Technology Type
Investigation: _I_T23_SxF
Study: _S_P5_SxFmycelia
Short Name: qRTPCRMothPheromonesEnzymes-Q-RT-PCR Assay Class: WET Assay Type: Q-RT-PCR Title: qRT-PCR Analysis of enzymes AtrD11 and HarFar in Penicillium digitatum transformants Description: Two Penicillium digitatum transformants were selected that showed integration of the two genes AtrD11 and HarFar (PCR test) but not moth pheromone production (GC-MS, analysed in CEQA facilities). These two transformants were then assayed in their expression of the transgenes by qRT-PCR. pISA Assay creation ...
Submitter: Marko Petek
Assay type: Experimental Assay Type
Technology type: Technology Type
Investigation: _I_T23_SxF
Study: _S_P1_ConstExp_Pdigitatum
Short Name: FS-GC-MS Assay Class: WET Assay Type: GC-MS Title: Full Scan analysis of SxF liquid samples Description: Full Scan analysis of the solvent extracts of SxF liquid samples pISA Assay creation date: 21-Sep-27 pISA Assay creator: Sandra Vacas Phenodata: None Featuredata: Data:
Submitter: Marko Petek
Assay type: Experimental Assay Type
Technology type: Technology Type
Investigation: _I_T23_SxF
Study: _S_P5_SxFliquids
Short Name: SRM-GC-MS Assay Class: WET Assay Type: GC-MS Title: SRM analysis of SxF liquid samples Description: GC-MS analysis in SRM mode of the solvent extracts of SxF liquid samples pISA Assay creation date: 21-Sep-27 pISA Assay creator: Sandra Vacas Phenodata: None Featuredata: Data:
Submitter: Marko Petek
Assay type: Experimental Assay Type
Technology type: Technology Type
Investigation: _I_T23_SxF
Study: _S_P5_SxFliquids
Short Name: FSM-GC-MS Assay Class: WET Assay Type: GC-MS Title: Full Scan analysis of SxF mycelium extracts Description: GC-MS analysis in full scan mode of SxF mycelium solvent extracts pISA Assay creation date: 21-Sep-27 pISA Assay creator: Sandra Vacas Phenodata: None Featuredata: Data:
Submitter: Marko Petek
Assay type: Experimental Assay Type
Technology type: Technology Type
Investigation: _I_T23_SxF
Study: _S_P5_SxFmycelia
Short Name: SRMM-GC-MS Assay Class: WET Assay Type: GC-MS Title: SRM analysis on SxF mycelium extracts Description: GC-MS analysis in SRM mode of SxF mycelium extracts pISA Assay creation date: 21-Sep-27 pISA Assay creator: Sandra Vacas Phenodata: None Featuredata: Data:
Submitter: Marko Petek
Assay type: Experimental Assay Type
Technology type: Technology Type
Investigation: _I_T23_SxF
Study: _S_P5_SxFmycelia
_p_SUSPHIRE/_I_T23_SxF/_S_P5_SxFmycelia/_A_SRMM-GC-MS/
Creator: Marko Petek
Submitter: Marko Petek
_p_SUSPHIRE/_I_T23_SxF/_S_P5_SxFmycelia/_A_SRMM-GC-MS/
Creator: Marko Petek
Submitter: Marko Petek
_p_SUSPHIRE/_I_T23_SxF/_S_P5_SxFmycelia/_A_FSM-GC-MS/
Creator: Marko Petek
Submitter: Marko Petek
_p_SUSPHIRE/_I_T23_SxF/_S_P5_SxFmycelia/_A_FSM-GC-MS/
Creator: Marko Petek
Submitter: Marko Petek
_p_SUSPHIRE/_I_T23_SxF/_S_P5_SxFmycelia/_A_SRMM-GC-MS/
Creator: Marko Petek
Submitter: Marko Petek
_p_SUSPHIRE/_I_T23_SxF/_S_P5_SxFmycelia/_A_SRMM-GC-MS/
Creator: Marko Petek
Submitter: Marko Petek
_p_SUSPHIRE/_I_T23_SxF/_S_P5_SxFmycelia/
Creator: Marko Petek
Submitter: Marko Petek
_p_SUSPHIRE/_I_T23_SxF/_S_P5_SxFliquids/_A_SRM-GC-MS/
Creator: Marko Petek
Submitter: Marko Petek
_p_SUSPHIRE/_I_T23_SxF/_S_P5_SxFmycelia/_A_FSM-GC-MS/
Creator: Marko Petek
Submitter: Marko Petek
_p_SUSPHIRE/_I_T23_SxF/_S_P5_SxFmycelia/_A_FSM-GC-MS/
Creator: Marko Petek
Submitter: Marko Petek
_p_SUSPHIRE/_I_T23_SxF/_S_P5_SxFliquids/_A_SRM-GC-MS/
Creator: Marko Petek
Submitter: Marko Petek
_p_SUSPHIRE/_I_T23_SxF/_S_P5_SxFliquids/_A_SRM-GC-MS/
Creator: Marko Petek
Submitter: Marko Petek
_p_SUSPHIRE/_I_T23_SxF/_S_P5_SxFliquids/
Creator: Marko Petek
Submitter: Marko Petek
_p_SUSPHIRE/_I_T23_SxF/_S_P5_SxFliquids/_A_FS-GC-MS/
Creator: Marko Petek
Submitter: Marko Petek
_p_SUSPHIRE/_I_T23_SxF/_S_P5_SxFliquids/_A_FS-GC-MS/
Creator: Marko Petek
Submitter: Marko Petek
_p_SUSPHIRE/_I_T23_SxF/_S_P5_SxFliquids/_A_SRM-GC-MS/
Creator: Marko Petek
Submitter: Marko Petek
_p_SUSPHIRE/_I_T23_SxF/_S_P5_SxFliquids/_A_FS-GC-MS/
Creator: Marko Petek
Submitter: Marko Petek
_p_SUSPHIRE/_I_T23_SxF/_S_P5_SxFliquids/_A_FS-GC-MS/
Creator: Marko Petek
Submitter: Marko Petek
_p_SUSPHIRE/_I_T23_SxF/_S_P5_SxFliquids/
Creator: Marko Petek
Submitter: Marko Petek
_p_SUSPHIRE/_I_T23_SxF/_S_P5_SxFliquids/
Creator: Marko Petek
Submitter: Marko Petek
_p_SUSPHIRE/_I_T23_SxF/
Creator: Marko Petek
Submitter: Marko Petek
_p_SUSPHIRE/_I_T23_SxF/
Creator: Marko Petek
Submitter: Marko Petek
_p_SUSPHIRE/_I_T23_SxF/_S_P5_SxFliquids/_A_FS-GC-MS/
Creator: Marko Petek
Submitter: Marko Petek
_p_SUSPHIRE/_I_T23_SxF/_S_P5_SxFliquids/_A_SRM-GC-MS/
Creator: Marko Petek
Submitter: Marko Petek
_p_SUSPHIRE/_I_T23_SxF/_S_P5_SxFmycelia/_A_FSM-GC-MS/
Creator: Marko Petek
Submitter: Marko Petek
_p_SUSPHIRE/_I_T23_SxF/_S_P5_SxFmycelia/_A_SRMM-GC-MS/
Creator: Marko Petek
Submitter: Marko Petek
_p_SUSPHIRE/_I_T23_SxF/
Creator: Marko Petek
Submitter: Marko Petek
