_A_qRTPCRMothPheromonesEnzymes-Q-RT-PCR
Short Name: qRTPCRMothPheromonesEnzymes-Q-RT-PCR Assay Class: WET Assay Type: Q-RT-PCR Title: qRT-PCR Analysis of enzymes AtrD11 and HarFar in Penicillium digitatum transformants Description: Two Penicillium digitatum transformants were selected that showed integration of the two genes AtrD11 and HarFar (PCR test) but not moth pheromone production (GC-MS, analysed in CEQA facilities). These two transformants were then assayed in their expression of the transgenes by qRT-PCR. pISA Assay creation date: 2021-01-20 pISA Assay creator: ElenaMG Phenodata: ../../phenodata_20210120 Featuredata: Data:
SEEK ID: https://fairdomhub.org/assays/1746
Experimental assay
Projects: SUSPHIRE - Sustainable Bioproduction of Pheromones for Insect Pest Contr...
Investigation: _I_T23_SxF
Study: _S_P1_ConstExp_Pdigitatum
Assay position:
Assay type: Experimental Assay Type
Technology type: Technology Type
Organisms: No organisms
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Created: 12th Apr 2022 at 16:01
Last updated: 7th Nov 2024 at 10:04
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https://orcid.org/0000-0003-3644-7827PhD in Biotechnology, Research Associate at Department of Biotechnology and Systems Biology, National Institute of Biology
National Institute of Biology, Department of Biotechnology and Systems Biology projects
Projects: HYp - Spatiotemporal analysis of hypersensitive response to Potato virus Y in potato, pISA-tree, MOA - Multiomics analysis of potato response to Potato virus Y (PVY) infection, SUSPHIRE - Sustainable Bioproduction of Pheromones for Insect Pest Control in Agriculture, INDIE - Biotechnological production of sustainable indole, _p_stRT, ADAPT - Accelerated Development of multiple-stress tolerAnt PoTato, _p_RNAinVAL, tst, tst2, Playground
Web page: http://www.nib.si/eng/index.php/departments/department-of-biotechnology-and-systems-biology
SUSPHIRE aims to provide a sustainable, low-cost biomanufacturing platform for the commercial production of insect pheromones.
Programme: NIBSys
Public web page: http://susphire.info/susphireproject/
Start date: 1st Apr 2018
End date: 30th Sep 2021
Short Name: T23_SxF Title: Moth pheromone production in filamentous fungi SxF Description: Demonstration of moth pheromone production in filamentous fungi by solvent extractions of liquid cultures and mycelial biomass and GC-MS/MS analysis. Constitutive and inducible expression of moth pheromones in filamentous fungi Penicillium species will be developed using a dCas9-based copper inducible system. An initial proof of concept will also be performed in P. digitatum using constitutive promoters. ...
Submitter: Marko Petek
Studies: Investigation files, _S_P1_ConstExp_Pdigitatum, _S_P5_SxFliquids, _S_P5_SxFmycelia
Assays: _A_FS-GC-MS, _A_FSM-GC-MS, _A_SRM-GC-MS, _A_SRMM-GC-MS, _A_qRTPCRMothPheromonesEnzymes-Q-RT-PCR, _I_T23_SxF-files, _S_P1_ConstExp_Pdigitatum-files, _S_P5_SxFliquids-files, _S_P5_SxFmycelia-files
Short Name: P1_ConstExp_Pdigitatum Title: Constitutive expression of moth pheromone pathway in Penicillium digitatum. Description: For this Proof of Concept, the three enzymes of the moth pheromones route were assembled in TUs using fungal promoters and terminators, and transformed into Penicillium digitatum. Pheromone production of the selected transformants will be assessed via GC-MS. Raw Data: pISA Study creation date: 2020-18-04 pISA Study creator: ElenaMG Principal investigator: Diego Orzaez ...
Submitter: Marko Petek
Investigation: _I_T23_SxF
Assays: _A_qRTPCRMothPheromonesEnzymes-Q-RT-PCR, _S_P1_ConstExp_Pdigitatum-files
_p_SUSPHIRE/_I_T23_SxF/_S_P1_ConstExp_Pdigitatum/_A_qRTPCRMothPheromonesEnzymes-Q-RT-PCR/
_p_SUSPHIRE/_I_T23_SxF/_S_P1_ConstExp_Pdigitatum/_A_qRTPCRMothPheromonesEnzymes-Q-RT-PCR/
_p_SUSPHIRE/_I_T23_SxF/_S_P1_ConstExp_Pdigitatum/_A_qRTPCRMothPheromonesEnzymes-Q-RT-PCR/
