Selection of complemented transgenic lines

The reporter fusion constructs expressing clock proteins fused to NanoLUC or firefly FLUC were transformed into the cognate, clock-mutant host plants. Each host also contained a transcriptional FLUC fusion that was used to score the circadian period of each transgenic line in constant light. Transformants that expressed a functionally normal level of clock protein were selected by choosing lines that complemented the mutant's period defect back close to the wild type period. Note that the reporters varied among the mutant host lines. Hence the rhythms in wild type controls are not expected to be the same in all cases, and separate controls are required for each clock protein/mutant combination, all in the Col-0 background.

Each line below is linked to the cognate, public experiment record in the BioDare2 repository, because FAIRDomHub is not accepting these links in the Data File recors.

LHY or CCA1 in cca1-1/lhy-11 double mutant, and TOC1 in toc1-2 mutant - https://biodare2.ed.ac.uk/experiment/11139

PRR7 in prr7-3 prr9-1 double mutant - https://biodare2.ed.ac.uk/experiment/10848

LUX in lux-2 mutant - https://biodare2.ed.ac.uk/experiment/11043, also tested by hypocotyl elongation.

Only ELF3 in elf3-2 mutant was not tested by period complementation but by hypocotyl elongation assays, please see Supp Fig 4g in the paper.