Data Files & Model Data

The Deutsche Forschungsgemeinschaft (DFG) is the self-governing organisation for science and research in Germany. It serves all branches of science and the humanities. In organisational terms, the DFG is an association under private law. Its membership consists of German research universities, non-university research institutions, scientific associations and the Academies of Science and the Humanities.

Cyanobacteria are the only prokaryotes performing oxygenic photosynthesis. Theyhad and have tremendous influence on the biogeochemical cycles on Earth. Recently,cyanobacteria are increasingly investigated as cell factories for a sustainableeconomy. Despite their global, environmental and rising economic importance, ourknowledge on the regulation of their primary metabolism is fragmented.Cyanobacteria switch between photoautotrophic and heterotrophic modes ofmetabolism during day/night cycles or ...

Summary Glycogen is the principal carbon reserve in Synechocystis sp. PCC 6803. We reconstituted its biosynthetic pathway in vitro—GlgC (Glucose-1-phosphate adenylyltransferase), two glycogen synthase isoenzymes (GlgA1, GlgA2) and the branching enzyme GlgB—to define how supply, polymerisation and branching set flux and product structure. GlgA2 shows higher specific activity and cooperates with GlgB-generated branched primers, whereas GlgA1 has higher substrate affinity and responds more to primer ...

Model CAB — README

Overview. Model CAB captures the coupled GlgC → GlgA (+ GlgB primer assistance) system under 3‑PGA/Pi regulation. The model exposes GlgC supply, GlgA demand, and a primer effectiveness term from GlgB; it also provides a Pi response calibrated to 2 mM 3‑PGA and helper utilities for GlgA1/GlgA2.

Key parameters (assay context) • GlgA ceilings (Assay B): GlgA1 = 0.49 U·mg⁻¹; GlgA2 = 0.64 U·mg⁻¹. • GlgA Michaelis constants (Assay A; ADP‑Glc): GlgA1 Kₘ = 0.152 mM; GlgA2 Kₘ = 0.219 ...

Use to generate all figures and perform analyses.

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