The soluble protein fraction was subjected to shotgun proteomics (i..e. in-solution digest) applying nanoLC ESI-Iontrap MS/MS and only detections in at least two replicates per condition and bacterium were considered for subsequent analyses. Correspondingly peptide count data was compiled per condition and strain studied.
SEEK ID: https://fairdomhub.org/assays/2320
Experimental assay
Projects: Proteogneomics of Desulfobacteraceae members
Investigation: Proteogenomic analyses of Desulfobacteraceae members
Study: Proteogenomic analyses of Desulofbacteraceae members
Assay position:
Assay type: Protein Expression Profiling
Technology type: Liquid Chromatography-tandom Mass Spectrometry
Organisms: Desulfobacula toluolica, Desulfococcus multivorans, Desulfonema limicola, Desulfonema magnum, Desulforapulum autotrophicum, Desulfosarcina variabilis
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Created: 11th Apr 2024 at 07:20
Last updated: 29th Oct 2024 at 12:45
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Projects: EbN1 Systems Biology, Heat stress response of the red-tide dinoflagellate Prorocentrum cordatum, The nucleus of Prorocentrum cordatum, Conspicuous chloroplast with LHC‒PSI/II‒megacomplex and diverse PBPs in the marine dinoflagellate Prorocentrum cordatum, Proteogneomics of Desulfobacteraceae members
Institutions: Carl von Ossietzky University of Oldenburg Institute for Chemistry and Biology of the Marine Environment (ICBM)
https://orcid.org/0000-0001-7253-3373Projects: Proteogneomics of Desulfobacteraceae members
Web page: Not specified
Programme: Independent Project
Public web page: Not specified
The family of Desulfobacteraceae comprises mtabolically versatile, completely oxidizing, sulfate-reducing bacteria that play an important role in the global carbon- and sulfor-cycles, mainly in the marine realm. In this study, six different species of Desulfobacteraceae were studied by comparative proteogenomics, i.e. Desulfobacula toluolica Tol2, Desulfococcus multivorans 1be1, Desulfonema limicola, Desulfonema magnum, Desulfosarcina variabilis 3be13 and Desulfobacterium autotrophicum ...
Submitter: Lars Wöhlbrand
Investigation: Proteogenomic analyses of Desulfobacteraceae me...
Assays: Membrane protein-enriched fraction proteomics of Desulfobacteraceae members, Shotgun proteomics of Desulfobacteraceae members
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