Karina Dale

Coastal zones and oceans constitute an essential fundament for Norway’s history as well as current economy, providing ecosystem services for fisheries, aquaculture, transport, tourism, and recreation. The petroleum activities in Norwegian waters have been crucial for Norway’s financial growth and in financing the Norwegian welfare state. As the pressure on the oceans continuously increases, both the petroleum industry and the seafood industries have acknowledged that awareness and actions are ...

Aim: To investigate whether Atlantic cod that feed close to aquatic breeding facilities are affected by chlorpyrifos-methyl. Feeding experiment with chlorpyrifos-methyl, an organophosphorous pesticide detected in plant based salmon feed. Based on previous experiments using salmon.

Doses: 0, 0.5, 5.0, 25 mg/kg) chlorpyrifos-methyl. Duration: 30 days Set-up: Three tanks per treatment (12 in total)

Samples include: Liver, plasma, bile, brain. Analysis include:

• Have RNAseq and metabolomics from 36 ...

Location: UiB Date: 8-20 June 2017 Compounds tested: WY-14,643 (PPAR alpha agonist), GW501516 (PPAR beta agonist) No. of test groups: 5 (Control 1: DMSO/saline/PEG, WY-14.643 High (40 mg/kg), WY-14.643 Low (4.0 mg/kg), GW501516 High (4.0 mg/kg), GW501516 Low (0.4 mg/kg)) No. of fish per group: 22

Aim: *To investigate effects on lipid metabolism in Atlantic cod ( Gadus morhua) mediated by peroxisome proliferator-activated receptors (Ppars) by in vivo exposure to two mammalian PPAR agonists.

BERGEN PUBLICATION Location: UiB Duration: 14 days, injections at day 0 and day 7 Sampling dates: 29-30 March 2017 Compounds tested: Polycyclic aromatic hydrocarbons (PAHs)/ Per- and polyfluoroalkyl substances (PFAS) No. of test groups: 10 No. of fish per group: 21-22 No. of dead fish:

Aim: *To investigate biological responses in Atlantic cod ( Gadus morhua) after in vivo exposure to a mixture of PAHs and PFASs, either singly or combined, at low (1x) or high (20x) doses. *PFAS concentrations were ...

This study involves all data gathered from the Kollevåg study - studying environmental pollution at a capped waste disposal site in Kollevåg, Askøy.

Cod were caged in Kollevåg (Stations 1, 2 and 3 - from inner to outer parts) and at a reference location (Ref station) for a period of six weeks, from 2nd September 2016, to 17-18th October 2016.

Information about the fish from the Kollevåg study

Includes physiological informations regarding the fish samples: Weight, length, gender, date sampled, comments regarding physical apperance, in addition to calculations of condition factor (K), hepatosomatic index (HSI) and gonadosomatic index (GSI).

Enzyme activity of glutathione-s-transferase (Gst) and catalase (Cat) in Kollevåg samples

Concentrations of steroid hormones E2 and T in female cod plasma from Kollevåg fish

Chemical analyses in Kollevåg includes sediment contaminant concentrations, concentrations of various contaminants in cod liver and concentrations of PAH metabolites in cod bile.

Western blot results for Kollevåg samples.

Analyses of gene expression: qPCR analyses in liver (relative expression) and ovaries (absolute expression).

TBARS assay measured oxidative stress as levels of malondialdehyde in samples. Performed in cod liver samples.

Biosense Vtg assay measuring concentrations of vitellogenin in cod blood plasma

Enzyme activity of the oxidative stress enzymes glutathione S-transferase (Gst) and catalase (Cat) measured in cod liver

Concentration of the egg yolk precursor protein vitellogenin in cod plasma

Gene expression of cyp1a and acox1 measured by qPCR in liver of Atlantic cod exposed to PAHs and PFASs

Semi-quantitative determination of Cyp1a protein amount in cod liver, using the ELISA method.

Fish weight (start - end), length, total growth and specific growth rate (SGR)

Transcriptomics results from liver of cod exposed to chlorpyrifos-methyl

Global metabolomics screening of 36 liver samples from Atlantic cod exposed to chlorpyrifos-methyl for 30 days.

Cyp1a activity (=EROD) measured in liver samples of cod exposed to chlorpyrifos-methyl

Concentrations of chlorpyrifos-methyl and its metabolite TCP in cod liver and bile quantified using gas chromatography

Measurement of plasma parameters: activities of cholinesterase, alanine aminotransferase, aspartate aminotransferase + cortisol and total protein levels in cod exposed to chlorpyrifos-methyl

Measurement of DNA fragmentation using the Comet Assay, in white blood cells of Atlantic cod exposed to PAHs and PFASs

Quantification of CPM and TCP concentrations in cod liver and bile using gas chromatography

Levels of cortisol+total protein and activities of cholinesterase, alanine aminotransferase (ALAT) and aspartate aminotransferase (ASAT) in plasma of cod exposed to chlorpyrifos-methyl

EROD activity in liver of cod exposed to chlorpyrifos-methyl

Overview of metabolomics results from liver of cod exposed to chlorpyrifos-methyl

Biometrics of IVN fish exposed to chlorpyrifos-methyl

Information connecting RNA seq fastq-files to corresponding fish ID/exposure regime

Data file containing protein ID and pre-processed proteomic data, in addition to protein groups (peptide counts etc) determined in cod liver of cod exposed to PAH and PFAS mixtures.

Results of lipid species (expressed in pmol) determined in cod liver microsomes exposed to PAH and PFAS

Concentrations of PFAS in liver and PAH metabolites in bile of cod exposed to mixtures of PAHs and PFASs

Tail intensities (%) measured by the Comet Assay as indication of DNA fragmentation in blood cells of Atlantic cod exposed to PAHs and PFASs

Measured in cod liver by the ELISA method, in Atlantic cod exposed to PAHs and PFASs

Mean normalized expression (MNE) levels of cyp1a and acox1 measured in liver of Atlantic Cod exposed to PAH and PFAS mixtures

Concentrations of the egg yolk precursor protein vitellogenin (Vtg) in cod plasma - 10 samples from each exposure group, IV1 experiment

Measured in liver of Atlantic cod exposed to PAHs and PFASs

Biometrics of IV1 fish exposed to PAH and PFAS

Concentrations of vitellogenin in blood plasma in cod caged in Kollevåg and at reference location.

Measurement of oxidative stress (TBARS) assay in liver of cod caged in Kollevåg and at reference location.

Gene expression data of genes involved in steroidogenic pathways, measured in cod ovaries.

Gene expression data of various genes from cod liver.

Relative protein expression of the proteins Cyp3a and Mt in cod from the Kollevåg study

Concentrations of PAH metabolites in bile and PFAS parent compounds in liver

Measurement of tail intensities in the Comet Assay - used in in vivo 1

Measurement of cholinesterase, ALAT and ASAT activities + cortisol and total protein levels - in vivo studies

Template for chemical quantification of CPM and TCP in liver and bile.

Template for biometrics for IV-nord experiment

Template for EROD activity measured in exposure studies

Semi-quantitative measurement of Cyp1a protein amount using the ELISA methods. Cyp1a amount is expressed as absorbance values at 450 nm.

Concentration of vitellogenin measured in cod blood plasma - in vivo studies

Enzyme activity of glutathione S-transferase and catalase in in vivo exposure studies

Concentrations of vitellogenin in blood plasma measured in field studies

TBARS assay: Measurement of oxidative stress

Gene expression data of cod liver including the genes cyp1a, cat, hsp70, gsta3b, fabp7, fasn, acly, and scd.

qPCR results using absolute quantification, genes involved in steroidogenic pathways

Concentrations of contaminants in cod liver

Template for relative protein expression of Cyp3a and MT in field studies

Concentrations of estradiol (E2) and testosterone (T) in cod plasma in field studies (groups defined by stations).

Template for in vivo biometrics data

Concentrations of PAH metabolites (1-OH-pyr, 1-OH-phen, 3-OH-BaP) in bile of fish at different stations

Template for biometry data for field studies such as Kollevåg, Oslofjord etc.