In vivo Nord 1: Chlorpyrifos-methyl

Aim: To investigate whether Atlantic cod that feed close to aquatic breeding facilities are affected by chlorpyrifos-methyl. Feeding experiment with chlorpyrifos-methyl, an organophosphorous pesticide detected in plant based salmon feed. Based on previous experiments using salmon.

Doses: 0, 0.5, 5.0, 25 mg/kg) chlorpyrifos-methyl. Duration: 30 days Set-up: Three tanks per treatment (12 in total)

Samples include: Liver, plasma, bile, brain. Analysis include:

• Have RNAseq and metabolomics from 36 liver samples
• Plasma cholinesterase, cortisol, ASAT, ALAT, and total protein (how many?)
• EROD on liver samples (how many?)
• Chemical levels of chlorpyrifos-methyl and main metabolite TCP in liver and bile (how many?)
• Hypoxia stress test (how many?)