In vivo Nord 1: Chlorpyrifos-methyl

Aim: To investigate whether Atlantic cod that feed close to aquatic breeding facilities are affected by chlorpyrifos-methyl. Feeding experiment with chlorpyrifos-methyl, an organophosphorous pesticide detected in plant based salmon feed. Based on previous experiments using salmon.

Doses: 0, 0.5, 5.0, 25 mg/kg) chlorpyrifos-methyl. Duration: 30 days Set-up: Three tanks per treatment (12 in total)

Samples include: Liver, plasma, bile, brain. Analysis include: - Have RNAseq and metabolomics from 36 liver samples - Plasma cholinesterase, cortisol, ASAT, ALAT, and total protein (36 samples) - EROD on liver samples (36 samples) - Chemical levels of chlorpyrifos-methyl and main metabolite TCP in liver and bile (36 samples) - Hypoxia stress test (72 samples)

DOI: None

Zenodo URL: None

Created at: 30th May 2021 at 23:30

Contents

Fish biometrics in vivo Nord

Fish weight (start - end), length, total growth and specific growth rate (SGR)

In vivo Nord 1 biometrics

Biometrics of IVN fish exposed to chlorpyrifos-methyl

  • In vivo Nord 1 biometrics.xlsx

Transcriptomics

Transcriptomics results from liver of cod exposed to chlorpyrifos-methyl

In vivo Nord 1 biometrics

Biometrics of IVN fish exposed to chlorpyrifos-methyl

  • In vivo Nord 1 biometrics.xlsx

File name information for RNA seq data

Information connecting RNA seq fastq-files to corresponding fish ID/exposure regime

  • RNA-seq file names.xlsx

Effects of agricultural pesticides in aquafeeds on wild fish feeding on leftover pellets near fish farms

The four treatment groups were Control (0 mg CPM/kg, with DMSO), 0.5 mg CPM/kg, 4.2 mg CPM/kg and 23.2 mg CPM/kg. Each treatment group consisted of 9 fish. These fish were sampled from 3 different tanks per treatment, with n=9 per treatment. A total of 36 samples were sequenced. For each sample, about 50 million 150 bp paired-end reads were generated.

  • acc.cgi?acc=GSE133594

In_Vivo_Nord_1_Olsvik - reads

No description specified

  • sbi.xhtml?ref=NjU3Mzc6Njc3MTE6cmVhZHM=

Metabolomics

Global metabolomics screening of 36 liver samples from Atlantic cod exposed to chlorpyrifos-methyl for 30 days.

Metabolomics_results

Overview of metabolomics results from liver of cod exposed to chlorpyrifos-methyl

  • Metabolomics_liver.xlsx

In vivo Nord 1 biometrics

Biometrics of IVN fish exposed to chlorpyrifos-methyl

  • In vivo Nord 1 biometrics.xlsx

EROD activity

Cyp1a activity (=EROD) measured in liver samples of cod exposed to chlorpyrifos-methyl

In vivo Nord 1 biometrics

Biometrics of IVN fish exposed to chlorpyrifos-methyl

  • In vivo Nord 1 biometrics.xlsx

Chemical analyses

Concentrations of chlorpyrifos-methyl and its metabolite TCP in cod liver and bile quantified using gas chromatography

CPM and TCP concentrations in liver and bile

Quantification of CPM and TCP concentrations in cod liver and bile using gas chromatography

  • CPM TCP-chemistry_IVnord.xlsx

In vivo Nord 1 biometrics

Biometrics of IVN fish exposed to chlorpyrifos-methyl

  • In vivo Nord 1 biometrics.xlsx

Plasma parameters

Measurement of plasma parameters: activities of cholinesterase, alanine aminotransferase, aspartate aminotransferase + cortisol and total protein levels
in cod exposed to chlorpyrifos-methyl

Plasma parameters

Levels of cortisol+total protein and activities of cholinesterase, alanine aminotransferase (ALAT) and aspartate aminotransferase (ASAT) in plasma of cod exposed to chlorpyrifos-methyl

  • Plasma measurements.xlsx

In vivo Nord 1 biometrics

Biometrics of IVN fish exposed to chlorpyrifos-methyl

  • In vivo Nord 1 biometrics.xlsx
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Created: 30th May 2021 at 23:30

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