In vivo Nord 1: Chlorpyrifos-methyl
DownloadAim: To investigate whether Atlantic cod that feed close to aquatic breeding facilities are affected by chlorpyrifos-methyl. Feeding experiment with chlorpyrifos-methyl, an organophosphorous pesticide detected in plant based salmon feed. Based on previous experiments using salmon.
Doses: 0, 0.5, 5.0, 25 mg/kg) chlorpyrifos-methyl. Duration: 30 days Set-up: Three tanks per treatment (12 in total)
Samples include: Liver, plasma, bile, brain. Analysis include:
- Have RNAseq and metabolomics from 36 liver samples
- Plasma cholinesterase, cortisol, ASAT, ALAT, and total protein (36 samples)
- EROD on liver samples (36 samples)
- Chemical levels of chlorpyrifos-methyl and main metabolite TCP in liver and bile (36 samples)
- Hypoxia stress test (72 samples)
DOI: None
Zenodo URL: None
Created at: 30th May 2021 at 21:30
Fish biometrics in vivo Nord
Fish weight (start - end), length, total growth and specific growth rate (SGR)
In vivo Nord 1 biometrics
Biometrics of IVN fish exposed to chlorpyrifos-methyl
- In vivo Nord 1 biometrics.xlsx
Transcriptomics
Transcriptomics results from liver of cod exposed to chlorpyrifos-methyl
In vivo Nord 1 biometrics
Biometrics of IVN fish exposed to chlorpyrifos-methyl
- In vivo Nord 1 biometrics.xlsx
File name information for RNA seq data
Information connecting RNA seq fastq-files to corresponding fish ID/exposure regime
- RNA-seq file names.xlsx
Effects of agricultural pesticides in aquafeeds on wild fish feeding on leftover pellets near fish farms
The four treatment groups were Control (0 mg CPM/kg, with DMSO), 0.5 mg CPM/kg, 4.2 mg CPM/kg and 23.2 mg CPM/kg. Each treatment group consisted of 9 fish. These fish were sampled from 3 different tanks per treatment, with n=9 per treatment. A total of 36 samples were sequenced. For each sample, about 50 million 150 bp paired-end reads were generated.
- acc.cgi?acc=GSE133594
Metabolomics
Global metabolomics screening of 36 liver samples from Atlantic cod exposed to chlorpyrifos-methyl for 30 days.
Metabolomics_results
Overview of metabolomics results from liver of cod exposed to chlorpyrifos-methyl
- Metabolomics_liver.xlsx
In vivo Nord 1 biometrics
Biometrics of IVN fish exposed to chlorpyrifos-methyl
- In vivo Nord 1 biometrics.xlsx
EROD activity
Cyp1a activity (=EROD) measured in liver samples of cod exposed to chlorpyrifos-methyl
In vivo Nord 1 biometrics
Biometrics of IVN fish exposed to chlorpyrifos-methyl
- In vivo Nord 1 biometrics.xlsx
Chemical analyses
Concentrations of chlorpyrifos-methyl and its metabolite TCP in cod liver and bile quantified using gas chromatography
CPM and TCP concentrations in liver and bile
Quantification of CPM and TCP concentrations in cod liver and bile using gas chromatography
- CPM TCP-chemistry_IVnord.xlsx
In vivo Nord 1 biometrics
Biometrics of IVN fish exposed to chlorpyrifos-methyl
- In vivo Nord 1 biometrics.xlsx
Plasma parameters
Measurement of plasma parameters: activities of cholinesterase, alanine aminotransferase, aspartate aminotransferase + cortisol and total protein levels
in cod exposed to chlorpyrifos-methyl
Plasma parameters
Levels of cortisol+total protein and activities of cholinesterase, alanine aminotransferase (ALAT) and aspartate aminotransferase (ASAT) in plasma of cod exposed to chlorpyrifos-methyl
- Plasma measurements.xlsx
In vivo Nord 1 biometrics
Biometrics of IVN fish exposed to chlorpyrifos-methyl
- In vivo Nord 1 biometrics.xlsx
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Created: 30th May 2021 at 21:30
