PAH/PFAS mixture toxicity in vivo

BERGEN PUBLICATION Location: UiB Duration: 14 days, injections at day 0 and day 7 Sampling dates: 29-30 March 2017 Compounds tested: Polycyclic aromatic hydrocarbons (PAHs)/ Per- and polyfluoroalkyl substances (PFAS) No. of test groups: 10 No. of fish per group: 21-22 No. of dead fish:

Aim: *To investigate biological responses in Atlantic cod ( Gadus morhua) after in vivo exposure to a mixture of PAHs and PFASs, either singly or combined, at low (1x) or high (20x) doses. *PFAS concentrations were determined in cod liver, and PAH metabolites were measured in bile as a biomarker of PAH exposure. *Various biomarkers were determined at transcript and protein levels in the liver, and lipidomics and proteomics analyses were performed on liver tissue to study responses on lipid metabolism.

DOI: 10.15490/fairdomhub.1.study.753.1

Zenodo URL: None

Created at: 7th Jul 2020 at 07:36

Contents

Fish biometrics in vivo I

Includes physiological informations regarding the fish samples: Weight, length, gender, date sampled, comments regarding physical apperance, in addition to calculations of condition factor (K), hepatosomatic index (HSI) and gonadosomatic index (GSI).

In vivo-1 biometrics

Biometrics of IV1 fish exposed to PAH and PFAS

Biometrics_IV1.xlsx

Enzyme activity assays

Enzyme activity of the oxidative stress enzymes glutathione S-transferase (Gst) and catalase (Cat) measured in cod liver

In vivo-1 biometrics

Biometrics of IV1 fish exposed to PAH and PFAS

Biometrics_IV1.xlsx

Enzyme activities catalase and glutathione S-transferase

Measured in liver of Atlantic cod exposed to PAHs and PFASs

Enzyme activity Gst Cat_standardized.xlsx

Vitellogenin in plasma

Concentration of the egg yolk precursor protein vitellogenin in cod plasma

Vitellogenin concentrations in cod plasma - IV1

Concentrations of the egg yolk precursor protein vitellogenin (Vtg) in cod plasma - 10 samples from each exposure group, IV1 experiment

Vtg concentration cod plasma - IV1.xlsx

In vivo-1 biometrics

Biometrics of IV1 fish exposed to PAH and PFAS

Biometrics_IV1.xlsx

Liver gene expression (qPCR)

Gene expression of cyp1a and acox1 measured by qPCR in liver of Atlantic cod exposed to PAHs and PFASs

In vivo-1 biometrics

Biometrics of IV1 fish exposed to PAH and PFAS

Biometrics_IV1.xlsx

Liver gene expression cyp1a and acox1

Mean normalized expression (MNE) levels of cyp1a and acox1 measured in liver of Atlantic Cod exposed to PAH and PFAS mixtures

Gene expression liver_standardized.xlsx

ELISA Cyp1a liver

Semi-quantitative determination of Cyp1a protein amount in cod liver, using the ELISA method.

In vivo-1 biometrics

Biometrics of IV1 fish exposed to PAH and PFAS

Biometrics_IV1.xlsx

Cyp1a protein amount

Measured in cod liver by the ELISA method, in Atlantic cod exposed to PAHs and PFASs

Cyp1a protein amount ELISA_standardized.xlsx

Comet Assay

Measurement of DNA fragmentation using the Comet Assay, in white blood cells of Atlantic cod exposed to PAHs and PFASs

In vivo-1 biometrics

Biometrics of IV1 fish exposed to PAH and PFAS

Biometrics_IV1.xlsx

Comet assay

Tail intensities (%) measured by the Comet Assay as indication of DNA fragmentation in blood cells of Atlantic cod exposed to PAHs and PFASs

Comet assay_standardized.xlsx

Chemical analyses

Measurements of PAH metabolites in bile (1-OH-napthalene, 2-OH-naphthalene, 1-OH-phenanthrene, 1-OH-pyrene) and PFAS compounds in liver (PFOS, PFOA, PFNA, PFTrDa)
in Atlantic cod exposed to mixtures of PAHs and PFASs.