Systems toxicology of Atlantic cod

BERGEN PUBLICATION
Location: UiB
Duration: 14 days, injections at day 0 and day 7
Sampling dates: 29-30 March 2017
Compounds tested: Polycyclic aromatic hydrocarbons (PAHs)/ Per- and polyfluoroalkyl substances (PFAS)
No. of test groups: 10
No. of fish per group: 21-22
No. of dead fish:

Aim:
*To investigate biological responses in Atlantic cod ( Gadus morhua) after in vivo exposure to a mixture of PAHs and PFASs, either singly or combined, at low (1x) or high (20x) doses.
*PFAS concentrations were
...

To map genome wide transcriptome responses in Atlantic cod PCLS treated with BaP and EE2. See Yadetie et al., 2018.

This study involves all data gathered from the Kollevåg study - studying environmental pollution at a capped waste disposal site in Kollevåg, Askøy.

Cod were caged in Kollevåg (Stations 1, 2 and 3 - from inner to outer parts) and at a reference location (Ref station) for a period of six weeks, from 2nd September 2016, to 17-18th October 2016.

Concentration of the egg yolk precursor protein vitellogenin in cod plasma

Gene expression of cyp1a and acox1 measured by qPCR in liver of Atlantic cod exposed to PAHs and PFASs

Semi-quantitative determination of Cyp1a protein amount in cod liver, using the ELISA method.

Measurement of DNA fragmentation using the Comet Assay, in white blood cells of Atlantic cod exposed to PAHs and PFASs

Measurements of PAH metabolites in bile (1-OH-napthalene, 2-OH-naphthalene, 1-OH-phenanthrene, 1-OH-pyrene) and PFAS compounds in liver (PFOS, PFOA, PFNA, PFTrDa)
in Atlantic cod exposed to mixtures of PAHs and PFASs.

Includes physiological informations regarding the fish samples: Weight, length, gender, date sampled, comments regarding physical apperance, in addition to calculations of condition factor (K), hepatosomatic index (HSI) and gonadosomatic index (GSI).

Enzyme activity of the oxidative stress enzymes glutathione S-transferase (Gst) and catalase (Cat) measured in cod liver

RNA-seq data for In vivo 7 samples. Only 32 RNA samples from liver of male fish sequenced. Illumina poly(A)-RNA sequencing at UiB GCF, as in Yadetie et al., 2018.

Information about the fish from the Kollevåg study

Enzyme activity of glutathione-s-transferase (Gst) and catalase (Cat) in Kollevåg samples

Concentrations of steroid hormones E2 and T in female cod plasma from Kollevåg fish

Chemical analyses in Kollevåg includes sediment contaminant concentrations, concentrations of various contaminants in cod liver and concentrations of PAH metabolites in cod bile.

Western blot results for Kollevåg samples.

Analyses of gene expression: qPCR analyses in liver (relative expression) and ovaries (absolute expression).

TBARS assay measured oxidative stress as levels of malondialdehyde in samples.
Performed in cod liver samples.

Biosense Vtg assay measuring concentrations of vitellogenin in cod blood plasma

Concentrations of PFAS in liver and PAH metabolites in bile of cod exposed to mixtures of PAHs and PFASs

Tail intensities (%) measured by the Comet Assay as indication of DNA fragmentation in blood cells of Atlantic cod exposed to PAHs and PFASs

Measured in cod liver by the ELISA method, in Atlantic cod exposed to PAHs and PFASs

Mean normalized expression (MNE) levels of cyp1a and acox1 measured in liver of Atlantic Cod exposed to PAH and PFAS mixtures

Concentrations of the egg yolk precursor protein vitellogenin (Vtg) in cod plasma - 10 samples from each exposure group, IV1 experiment

Measured in liver of Atlantic cod exposed to PAHs and PFASs

Biometrics of IV1 fish exposed to PAH and PFAS

Concentrations of vitellogenin in blood plasma in cod caged in Kollevåg and at reference location.

Measurement of oxidative stress (TBARS) assay in liver of cod caged in Kollevåg and at reference location.

Gene expression data of genes involved in steroidogenic pathways, measured in cod ovaries.

Gene expression data of various genes from cod liver.

Relative protein expression of the proteins Cyp3a and Mt in cod from the Kollevåg study

Measurements of PAH metabolites in bile in fish caged in Kollevåg.

Concentrations of various contaminants in liver from cod caged in Kollevåg and at reference location.

Concentrations of E2 and T in cod plasma of female cod caged in Kollevåg measured by enzyme immunoassay.

Concentrations of metals, PAHs and PCBs in sediment samples from Kollevåg and reference site.

Measurement of Gst and Cat enzyme activity in liver of fish caged in Kollevåg.