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Systems toxicology of Atlantic cod

Overview

Coastal zones and oceans constitute an essential fundament for Norway’s history as well as current economy, providing ecosystem services for fisheries, aquaculture, transport, tourism, and recreation. The petroleum activities in Norwegian waters have been crucial for Norway’s financial growth and in financing the Norwegian welfare state. As the pressure on the oceans continuously increases, both the petroleum industry and the seafood industries have acknowledged that awareness and actions are required to maintain a healthy marine environment to secure Norway´s future marine bioeconomy. Marine industries must also comply with governmental and international regulations regarding sustainable practices of marine activities, and novel technologies are needed in order to efficiently cope with such future demands.

The goal of the dCod-project is to combine the competencies in environmental toxicology, biology, bioinformatics and mathematics across the traditional department boundaries, to create a deeper understanding of cods' adaptations and reactions to stressors in the environment. The projects aims to generate large amounts of experimental data to be the basis of mathematical models that can describe these responses based on different scenarios. Furthermore, the overall goal is to create a tool for environmental monitoring and risk assessment that can be used in assessing the impacts of for example the oil industry, sewage discharge into harbours and industrial discharge into Norwegian fjords.

The project is associated with the Centre for Digital Life Norway.

Programme: dCod 1.0: decoding systems toxicology of cod

SEEK ID: https://fairdomhub.org/projects/60

Public web page: http://www.uib.no/en/dcod

Organisms: Gadus morhua

FAIRDOM PALs: No PALs for this Project

Project created: 5th Feb 2017

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Libe Aranguren Abadía

Projects: Systems toxicology of Atlantic cod

Institutions: University of Bergen

Nello Blaser project_administratorprogramme_administrator

Projects: Systems toxicology of Atlantic cod

Institutions: University of Bergen

Morten Brun

Projects: Systems toxicology of Atlantic cod

Institutions: University of Bergen

Korbinian Bösl programme_administrator

Projects: Systems toxicology of Atlantic cod

Institutions: University of Bergen

https://orcid.org/0000-0003-0498-4273
Karina Dale project_administratorprogramme_administrator

Projects: Systems toxicology of Atlantic cod

Institutions: University of Bergen

Dorothy Dankel

Projects: Systems toxicology of Atlantic cod

Institutions: University of Bergen

https://orcid.org/0000-0002-8839-3333
Marta Eide

Projects: Systems toxicology of Atlantic cod

Institutions: University of Bergen

Shirin Fallahi

Projects: Systems toxicology of Atlantic cod

Institutions: University of Bergen

Anders Goksøyr project_administratorprogramme_administrator

Projects: Systems toxicology of Atlantic cod

Institutions: University of Bergen

https://orcid.org/0000-0003-4054-9842
Siri Øfsthus Goksøyr

Projects: Systems toxicology of Atlantic cod

Institutions: University of Bergen

https://orcid.org/0000-0003-0073-3282
Eileen Marie Hanna

Projects: Systems toxicology of Atlantic cod

Institutions: University of Bergen

Ketil Hylland

Projects: Systems toxicology of Atlantic cod

Institutions: University of Olso

https://orcid.org/0000-0001-5453-2932
Inge Jonassen

Projects: Systems toxicology of Atlantic cod

Institutions: University of Bergen

https://orcid.org/0000-0003-4110-0748
Odd André Karlsen

Projects: Systems toxicology of Atlantic cod

Institutions: University of Bergen

Essa Ahsan Khan

Projects: Systems toxicology of Atlantic cod

Institutions: Norwegian University of Science and Technology

Pål A. Olsvik

Projects: Systems toxicology of Atlantic cod

Institutions: University of Bergen

https://orcid.org/0000-0002-5641-9420
Sofie Söderström project_administrator

Projects: Systems toxicology of Atlantic cod

Institutions: University of Bergen

Zhanna Tairova project_administratorprogramme_administrator

Projects: Systems toxicology of Atlantic cod

Institutions: University of Olso

Kidane Tekle

Projects: Systems toxicology of Atlantic cod

Institutions: University of Bergen

https://orcid.org/0000-0002-4296-5068
Jon Olav Vik project_administratorasset_housekeeperasset_gatekeeperprogramme_administrator

Projects: GenoSysFat, DigiSal, FAIRDOM user meeting, SEEK tutorial for DigiSal, Systems toxicology of Atlantic cod

Institutions: Norwegian University of Life Sciences

https://orcid.org/0000-0002-7778-4515
Norwegian University of Life Sciences

Country:  Norway

City: N-1432 Ås

Web page: http://www.nmbu.no/en

Norwegian University of Science and Technology

Country:  Norway

City: Trondheim

Web page: http://www.ntnu.edu/

University of Bergen

Country:  Norway

City: Not specified

Web page: Not specified

University of Olso

Country:  Norway

City: Oslo

Web page: https://www.uio.no/

6 hidden items
Effects of BaP and EE2 treatments on transcriptome responses in PCLS
Systems toxicology of Atlantic cod

To map genome wide transcriptome responses in Atlantic cod PCLS treated with BaP and EE2. See Yadetie et al., 2018.

Person responsible: Fekadu Yadetie

Snapshots: No snapshots

Caging study - Kollevåg
Systems toxicology of Atlantic cod

This study involves all data gathered from the Kollevåg study - studying environmental pollution at a capped waste disposal site in Kollevåg, Askøy.

Cod were caged in Kollevåg (Stations 1, 2 and 3 - from inner to outer parts) and at a reference location (Ref station) for a period of six weeks, from 2nd September 2016, to 17-18th October 2016.

Person responsible: Karina Dale

Snapshots: Snapshot 1

DOI: 10.15490/fairdomhub.1.study.361.1

Effects of PCB 153 in Atlantic cod
Systems toxicology of Atlantic cod
No description specified

Person responsible: Fekadu Yadetie

Snapshots: No snapshots

23 hidden items
RNA-seq data for BaP and EE2 treated PCLS
Systems toxicology of Atlantic cod
No description specified

Contributor: Fekadu Yadetie

Assay type: RNA-seq

Technology type: Next generation sequencing

Snapshots: No snapshots

Liver RNA-seq data for in vivo VII
Systems toxicology of Atlantic cod

RNA-seq data for In vivo 7 samples. Only 32 RNA samples from liver of male fish sequenced. Illumina poly(A)-RNA sequencing at UiB GCF, as in Yadetie et al., 2018.

Contributor: Fekadu Yadetie

Assay type: RNA-seq

Technology type: Next generation sequencing

Snapshots: No snapshots

Vitellogenin in blood plasma
Systems toxicology of Atlantic cod

Biosense Vtg assay measuring concentrations of vitellogenin in cod blood plasma

Contributor: Karina Dale

Assay type: Experimental Assay Type

Technology type: Technology Type

Snapshots: No snapshots

TBARS: Oxidative stress
Systems toxicology of Atlantic cod

TBARS assay measured oxidative stress as levels of malondialdehyde in samples.
Performed in cod liver samples.

Contributor: Karina Dale

Assay type: Experimental Assay Type

Technology type: Technology Type

Snapshots: No snapshots

Gene expression
Systems toxicology of Atlantic cod

Analyses of gene expression: qPCR analyses in liver (relative expression) and ovaries (absolute expression).

Contributor: Karina Dale

Assay type: Experimental Assay Type

Technology type: Technology Type

Snapshots: No snapshots

Western blot - protein expression
Systems toxicology of Atlantic cod

Western blot results for Kollevåg samples.

Contributor: Karina Dale

Assay type: Experimental Assay Type

Technology type: Technology Type

Snapshots: No snapshots

Chemical analyses
Systems toxicology of Atlantic cod

Chemical analyses in Kollevåg includes sediment contaminant concentrations, concentrations of various contaminants in cod liver and concentrations of PAH metabolites in cod bile.

Contributor: Karina Dale

Assay type: Experimental Assay Type

Technology type: Technology Type

Snapshots: No snapshots

Enzyme immunoassay
Systems toxicology of Atlantic cod

Concentrations of steroid hormones E2 and T in female cod plasma from Kollevåg fish

Contributor: Karina Dale

Assay type: Experimental Assay Type

Technology type: Technology Type

Snapshots: No snapshots

Enzyme activity assays
Systems toxicology of Atlantic cod

Enzyme activity of glutathione-s-transferase (Gst) and catalase (Cat) in Kollevåg samples

Contributor: Karina Dale

Assay type: Experimental Assay Type

Technology type: Enzymatic Activity Measurements

Snapshots: No snapshots

Fish Biometrics
Systems toxicology of Atlantic cod

Information about the fish from the Kollevåg study

Contributor: Karina Dale

Assay type: Experimental Assay Type

Technology type: Technology Type

Snapshots: No snapshots

68 hidden items
PCLS_BaP-EE2_RNAseq - analysis
Systems toxicology of Atlantic cod
No description specified

Creator: Fekadu Yadetie

Contributor: Fekadu Yadetie

Open in NeLS
PCLS_BaP-EE2_RNAseq - reads
Systems toxicology of Atlantic cod
No description specified

Creator: Fekadu Yadetie

Contributor: Fekadu Yadetie

Open in NeLS
RNA-seq metadata for BaP and EE2 treated PCLS study
Systems toxicology of Atlantic cod
No description specified

Creator: Fekadu Yadetie

Contributor: Fekadu Yadetie

Vitellogenin_plasma_Kollevåg
Systems toxicology of Atlantic cod

Concentrations of vitellogenin in blood plasma in cod caged in Kollevåg and at reference location.

Creators: None

Contributor: Karina Dale

Download
TBARS_Kollevåg
Systems toxicology of Atlantic cod

Measurement of oxidative stress (TBARS) assay in liver of cod caged in Kollevåg and at reference location.

Creators: None

Contributor: Karina Dale

Download
qPCR liver_Kollevåg
Systems toxicology of Atlantic cod

Gene expression data of various genes from cod liver.

Creators: None

Contributor: Karina Dale

Download
qPCR ovaries_Kollevåg
Systems toxicology of Atlantic cod

Gene expression data of genes involved in steroidogenic pathways, measured in cod ovaries.

Creators: None

Contributor: Karina Dale

Download
Protein expression Cyp3a+Mt Western Blot_Kollevåg
Systems toxicology of Atlantic cod

Relative protein expression of the proteins Cyp3a and Mt in cod from the Kollevåg study

Creators: None

Contributor: Karina Dale

Download
Liver chemistry_Kollevåg
Systems toxicology of Atlantic cod

Concentrations of various contaminants in liver from cod caged in Kollevåg and at reference location.

Creators: None

Contributor: Karina Dale

Download
PAH metabolites in bile Kollevåg
Systems toxicology of Atlantic cod

Measurements of PAH metabolites in bile in fish caged in Kollevåg.

Creator: Karina Dale

Contributor: Karina Dale

Download
Sediment chemistry
Systems toxicology of Atlantic cod

Concentrations of metals, PAHs and PCBs in sediment samples from Kollevåg and reference site.

Creators: None

Contributor: Karina Dale

Download
Steroid hormones plasma Kollevåg
Systems toxicology of Atlantic cod

Concentrations of E2 and T in cod plasma of female cod caged in Kollevåg measured by enzyme immunoassay.

Creators: None

Contributor: Karina Dale

Download
Enzyme activity Gst+Cat_Kollevåg
Systems toxicology of Atlantic cod

Measurement of Gst and Cat enzyme activity in liver of fish caged in Kollevåg.

Creator: Karina Dale

Contributor: Karina Dale

Download
Kollevåg biometrics
Systems toxicology of Atlantic cod

Biometry data of fish from Kollevåg caging study

Creator: Karina Dale

Contributor: Karina Dale

Download
Count files
Systems toxicology of Atlantic cod
No description specified

Creator: Fatemeh Zamanzad Ghavidel

Contributor: Fatemeh Zamanzad Ghavidel

Download
Bam files
Systems toxicology of Atlantic cod
No description specified

Creator: Fatemeh Zamanzad Ghavidel

Contributor: Fatemeh Zamanzad Ghavidel

Download
FASTQ Paired
Systems toxicology of Atlantic cod
No description specified

Creator: Fatemeh Zamanzad Ghavidel

Contributor: Fatemeh Zamanzad Ghavidel

Download
Library preparation
Systems toxicology of Atlantic cod
No description specified

Creator: Fatemeh Zamanzad Ghavidel

Contributor: Fatemeh Zamanzad Ghavidel

Download
Samples for dCod
Systems toxicology of Atlantic cod
No description specified

Creator: Fatemeh Zamanzad Ghavidel

Contributor: Fatemeh Zamanzad Ghavidel

Download
45 hidden items
Modulation of Neuro-Dopamine Homeostasis in Juvenile Female Atlantic Cod ( Gadus morhua) Exposed to Polycyclic Aromatic Hydrocarbons and Perfluoroalkyl Substances.
Systems toxicology of Atlantic cod

Abstract (Expand)

The dopaminergic effect of PAH and PFAS mixtures, prepared according to environmentally relevant concentrations, has been studied in juvenile female Atlantic cod ( Gadus morhua). Benzo[a]pyrene, … dibenzothiophene, fluorene, naphthalene, phenanthrene, and pyrene were used to prepare a PAH mixture, while PFNA, PFOA, PFOS, and PFTrA were used to prepare a PFAS mixture. Cod were injected intraperitoneally twice, with either a low (1x) or high (20x) dose of each compound mixture or their combinations. After 2 weeks of exposure, levels of plasma 17beta-estradiol (E2) were significantly elevated in high PAH/high PFAS treated group. Brain dopamine/metabolite ratios (DOPAC/dopamine and HVA+DOPAC/dopamine) changed with E2 plasma levels, except for high PAH/low PFAS and low PAH/high PFAS treated groups. On the transcript levels, th mRNA inversely correlated with dopamine/metabolite ratios and gnrh2 mRNA levels. Respective decreases and increases of drd1 and drd2a after exposure to the high PAH dose were observed. Specifically, high PFAS exposure decreased both drds, leading to high plasma E2 concentrations. Other studied end points suggest that these compounds, at different doses and combinations, have different toxicity threshold and modes of action. These effects indicate potential alterations in the feedback signaling processes within the dopaminergic pathway by these contaminant mixtures.

Authors: Essa Ahsan Khan, L. B. Bertotto, Karina Dale, R. Lille-Langoy, Fekadu Yadetie, Odd André Karlsen, Anders Goksøyr, D. Schlenk, A. Arukwe

Date Published: 18th Jun 2019

Journal: Environ Sci Technol

Contaminant accumulation and biological responses in Atlantic cod (Gadus morhua) caged at a capped waste disposal site in Kollevag, Western Norway.
Systems toxicology of Atlantic cod

Abstract (Expand)

The aim of this study was to assess whether fish in Kollevag, a sheltered bay on the western coast of Norway, previously utilized as a waste disposal site, could be affected by environmental contaminants … leaking from the waste. Farmed, juvenile Atlantic cod (Gadus morhua) were caged for six weeks at three different locations in Kollevag bay and at one reference location. Sediments and cod samples (bile and liver) were analyzed for polychlorinated biphenyls (PCBs), organochlorine pesticides (OCPs), brominated flame retardants (BFRs), per-and polyfluoroalkyl substances (PFASs) and polycyclic aromatic hydrocarbon (PAH) metabolites, revealing a contamination gradient at the four stations. Furthermore, hepatosomatic index (HSI) and Fulton's condition factor (CF) were significantly lower in cod caged closest to the disposal site. Levels and activities of biomarker proteins, such as vitellogenin (Vtg), metallothionein (Mt), and biotransformation and oxidative stress enzymes, including cytochrome P450 1a and 3a (Cyp1a, Cyp3a), glutathione s-transferase (Gst) and catalase (Cat), were quantified in blood plasma and liver tissue. Hepatic Cat and Gst activities were significantly reduced in cod caged at the innermost stations in Kollevag, indicating modulation of oxidative stress responses. However, these results contrasted with reduced hepatic lipid peroxidation. Significant increases in transcript levels were observed for genes involved in lipid metabolism (fasn and acly) in cod liver, while transcript levels of ovarian steroidogenic enzyme genes such as p450scc, cyp19, 3beta-hsd and 20beta-hsd showed significant station-dependent increases. Cyp1a and Vtg protein levels were however not significantly altered in cod caged in Kollevag. Plasma levels of estradiol (E2) and testosterone (T) were determined by enzyme immunoassay (EIA) and showed elevated E2 levels, but only at the innermost station. We conclude that the bay of Kollevag did not fullfill adequate environmental condition based on environmental quality standards (EQSs) for chemicals in coastal waters. Following a six weeks caging period, environmental contaminants accumulated in cod tissues and effects were observed on biomarker responses, especially those involved in reproductive processes in cod ovary.

Authors: Karina Dale, M. B. Muller, Zhanna Tairova, Essa Ahsan Khan, K. Hatlen, M. Grung, Fekadu Yadetie, R. Lille-Langoy, Nello Blaser, H. J. Skaug, J. L. Lyche, A. Arukwe, Ketil Hylland, Odd André Karlsen, Anders Goksøyr

Date Published: 26th Feb 2019

Journal: Mar Environ Res

RNA-Seq analysis of transcriptome responses in Atlantic cod (Gadus morhua) precision-cut liver slices exposed to benzo[a]pyrene and 17alpha-ethynylestradiol.
Systems toxicology of Atlantic cod

Abstract (Expand)

Polycyclic aromatic hydrocarbons such as benzo[a]pyrene (BaP) that activate the aryl hydrocarbon receptor (Ahr) pathway, and endocrine disruptors acting through the estrogen receptor pathway are among … environmental pollutants of major concern. In this work, we exposed Atlantic cod (Gadus morhua) precision-cut liver slices (PCLS) to BaP (10nM and 1000nM), ethynylestradiol (EE2) (10nM and 1000nM), and equimolar mixtures of BaP and EE2 (10nM and 1000nM) for 48h, and performed RNA-Seq based transcriptome mapping followed by systematic bioinformatics analyses. Our gene expression analysis showed that several genes were differentially expressed in response to BaP and EE2 treatments in PCLS. Strong up-regulation of genes coding for the cytochrome P450 1a (Cyp1a) enzyme and the Ahr repressor (Ahrrb) was observed in BaP treated PCLS. EE2 treatment of liver slices strongly up-regulated genes coding for precursors of vitellogenin (Vtg) and eggshell zona pellucida (Zp) proteins. As expected, pathway enrichment and network analysis showed that the Ahr and estrogen receptor pathways are among the top affected by BaP and EE2 treatments, respectively. Interestingly, two genes coding for fibroblast growth factor 3 (Fgf3) and fibroblast growth factor 4 (Fgf4) were up-regulated by EE2 in this study. To our knowledge, the fgf3 and fgf4 genes have not previously been described in relation to estrogen signaling in fish liver, and these results suggest the modulation of the FGF signaling pathway by estrogens in fish. The signature expression profiles of top differentially expressed genes in response to the single compound (BaP or EE2) treatment were generally maintained in the expression responses to the equimolar binary mixtures. However, in the mixture-treated groups, BaP appeared to have anti-estrogenic effects as observed by lower number of differentially expressed putative EE2 responsive genes. Our in-depth quantitative analysis of changes in liver transcriptome in response to BaP and EE2, using PCLS tissue culture provides further mechanistic insights into effects of the compounds. Moreover, the analyses demonstrate the usefulness of PCLS in cod for omics experiments.

Authors: Fekadu Yadetie, Xiaokang Zhang, Eileen Marie Hanna, L. Aranguren-Abadia, Marta Eide, Nello Blaser, Morten Brun, Inge Jonassen, A. Goksoyr, Odd André Karlsen

Date Published: 22nd Jun 2018

Journal: Aquat Toxicol

Quantitative proteomics analysis reveals perturbation of lipid metabolic pathways in the liver of Atlantic cod (Gadus morhua) treated with PCB 153
Systems toxicology of Atlantic cod

Abstract (Expand)

PCB 153 is one of the most abundant PCB congeners detected in biological samples. It is a persistent compound that is still present in the environment despite the ban on production and use of PCBs in … the late 1970s. It has strong tendencies to bioaccumulate and biomagnify in biota, and studies have suggested that it is an endocrine and metabolic disruptor. In order to study mechanisms of toxicity, we exposed Atlantic cod (Gadus morhua) to various doses of PCB 153 (0, 0.5, 2 and 8 mg/kg body weight) for two weeks and examined the effects on expression of liver proteins using label-free quantitative proteomics. Label-free liquid chromatography-mass spectrometry analysis of the liver proteome resulted in the quantification of 1272 proteins, of which 78 proteins were differentially regulated in the PCB 153-treated dose groups compared to the control group. Functional enrichment analysis showed that pathways significantly affected are related to the lipid metabolism, cytoskeletal remodeling, cell cycle and cell adhesion. Importantly, the main effects appear to be on lipid metabolism, with up-regulation of enzymes in the de novo fatty acid synthesis pathway, consistent with previous transcriptomics results. Increased plasma triglyceride levels were also observed in the PCB 153 treated fish, in agreement with the induction of the lipogenic genes and proteins. The results suggest that PCB 153 perturbs lipid metabolism in the Atlantic cod liver. Elevated levels of lipogenic enzymes and plasma triglycerides further suggest increased synthesis of fatty acids and triglycerides.

Author: Fekadu Yadetie, Eystein Oveland, Anne Døskeland, Frode Berven Anders Goksøyr, Odd André Karlsen

Date Published: 1st Apr 2017

Journal: Aquatic Toxicology

Quantitative analyses of the hepatic proteome of methylmercury-exposed Atlantic cod (Gadus morhua) suggest oxidative stress-mediated effects on cellular energy metabolism
Systems toxicology of Atlantic cod

Abstract (Expand)

BACKGROUND: Methylmecury (MeHg) is a widely distributed environmental pollutant with considerable risk to both human health and wildlife. To gain better insight into the underlying mechanisms of … MeHg-mediated toxicity, we have used label-free quantitative mass spectrometry to analyze the liver proteome of Atlantic cod (Gadus morhua) exposed in vivo to MeHg (0, 0.5, 2 mg/kg body weight) for 2 weeks. RESULTS: Out of a toltal of 1143 proteins quantified, 125 proteins were differentially regulated between MeHg-treated samples and controls. Using various bioinformatics tools, we performed gene ontology, pathway and network enrichment analysis, which indicated that proteins and pathways mainly related to energy metabolism, antioxidant defense, cytoskeleton remodeling, and protein synthesis were regulated in the hepatic proteome after MeHg exposure. Comparison with previous gene expression data strengthened these results, and further supported that MeHg predominantly affects many energy metabolism pathways, presumably through its strong induction of oxidative stress. Some enzymes known to have functionally important oxidation-sensitive cysteine residues in other animals are among the differentially regulated proteins, suggesting their modulations by MeHg-induced oxidative stress. Integrated analysis of the proteomics dataset combined with previous gene expression dataset showed a more pronounced effect of MeHg on amino acid, glucose and fatty acid metabolic pathways, and suggested possible interactions of the cellular energy metabolism and antioxidant defense pathways. CONCLUSIONS: MeHg disrupts mainly redox homeostasis and energy generating metabolic pathways in cod liver. The energy pathways appear to be modulated through MeHg-induced oxidative stress, possibly mediated by oxidation sensitive enzymes.

Authors: Fekadu Yadetie, S. Bjorneklett, H. K. Garberg, E. Oveland, F. Berven, A. Goksoyr, O. A. Karlsen

Date Published: 9th Aug 2016

Journal: BMC Genomics

Liver transcriptome analysis of Atlantic cod (Gadus morhua) exposed to PCB 153 indicates effects on cell cycle regulation and lipid metabolism
Systems toxicology of Atlantic cod

Abstract (Expand)

BACKGROUND: Polychlorinated biphenyls (PCBs) are persistent organic pollutants (POPs) with harmful effects in animals and humans. Although PCB 153 is one of the most abundant among PCBs detected in … animal tissues, its mechanism of toxicity is not well understood. Only few studies have been conducted to explore genes and pathways affected by PCB 153 by using high throughput transcriptomics approaches. To obtain better insights into toxicity mechanisms, we treated juvenile Atlantic cod (Gadus morhua) with PCB 153 (0.5, 2 and 8 mg/kg body weight) for 2 weeks and performed gene expression analysis in the liver using oligonucleotide arrays. RESULTS: Whole-genome gene expression analysis detected about 160 differentially regulated genes. Functional enrichment, interactome, network and gene set enrichment analysis of the differentially regulated genes suggested that pathways associated with cell cycle, lipid metabolism, immune response, apoptosis and stress response were among the top significantly enriched. Particularly, genes coding for proteins in DNA replication/cell cycle pathways and enzymes of lipid biosynthesis were up-regulated suggesting increased cell proliferation and lipogenesis, respectively. CONCLUSIONS: PCB 153 appears to activate cell proliferation and lipogenic genes in cod liver. Transcriptional up-regulation of marker genes for lipid biosynthesis resembles lipogenic effects previously reported for persistent organic pollutants (POPs) and other environmental chemicals. Our results provide new insights into mechanisms of PCB 153 induced toxicity.

Authors: F. Yadetie, O. A. Karlsen, M. Eide, C. Hogstrand, A. Goksoyr

Date Published: 19th Jun 2014

Journal: BMC Genomics

Global transcriptome analysis of Atlantic cod (Gadus morhua) liver after in vivo methylmercury exposure suggests effects on energy metabolism pathways
Systems toxicology of Atlantic cod

Abstract (Expand)

Methylmercury (MeHg) is a widely distributed contaminant polluting many aquatic environments, with health risks to humans exposed mainly through consumption of seafood. The mechanisms of toxicity of … MeHg are not completely understood. In order to map the range of molecular targets and gain better insights into the mechanisms of toxicity, we prepared Atlantic cod (Gadus morhua) 135k oligonucleotide arrays and performed global analysis of transcriptional changes in the liver of fish treated with MeHg (0.5 and 2 mg/kg of body weight) for 14 days. Inferring from the observed transcriptional changes, the main pathways significantly affected by the treatment were energy metabolism, oxidative stress response, immune response and cytoskeleton remodeling. Consistent with known effects of MeHg, many transcripts for genes in oxidative stress pathways such as glutathione metabolism and Nrf2 regulation of oxidative stress response were differentially regulated. Among the differentially regulated genes, there were disproportionate numbers of genes coding for enzymes involved in metabolism of amino acids, fatty acids and glucose. In particular, many genes coding for enzymes of fatty acid beta-oxidation were up-regulated. The coordinated effects observed on many transcripts coding for enzymes of energy pathways may suggest disruption of nutrient metabolism by MeHg. Many transcripts for genes coding for enzymes in the synthetic pathways of sulphur containing amino acids were also up-regulated, suggesting adaptive responses to MeHg toxicity. By this toxicogenomics approach, we were also able to identify many potential biomarker candidate genes for monitoring environmental MeHg pollution. These results based on changes on transcript levels, however, need to be confirmed by other methods such as proteomics.

Authors: Fekadu Yadetie, O. A. Karlsen, A. Lanzen, K. Berg, P. Olsvik, C. Hogstrand, A. Goksoyr

Date Published: 30th Oct 2012

Journal: Aquat Toxicol

1 hidden item
16089
Systems toxicology of Atlantic cod

Type: Vitellogenin concentration in plasma field studies

Creators: None

Contributor: Karina Dale

16088
Systems toxicology of Atlantic cod

Type: Vitellogenin concentration in plasma field studies

Creators: None

Contributor: Karina Dale

16087
Systems toxicology of Atlantic cod

Type: Vitellogenin concentration in plasma field studies

Creators: None

Contributor: Karina Dale

16086
Systems toxicology of Atlantic cod

Type: Vitellogenin concentration in plasma field studies

Creators: None

Contributor: Karina Dale

16085
Systems toxicology of Atlantic cod

Type: Vitellogenin concentration in plasma field studies

Creators: None

Contributor: Karina Dale

16084
Systems toxicology of Atlantic cod

Type: Vitellogenin concentration in plasma field studies

Creators: None

Contributor: Karina Dale

16083
Systems toxicology of Atlantic cod

Type: Vitellogenin concentration in plasma field studies

Creators: None

Contributor: Karina Dale

16082
Systems toxicology of Atlantic cod

Type: Vitellogenin concentration in plasma field studies

Creators: None

Contributor: Karina Dale

16081
Systems toxicology of Atlantic cod

Type: Vitellogenin concentration in plasma field studies

Creators: None

Contributor: Karina Dale

16080
Systems toxicology of Atlantic cod

Type: Vitellogenin concentration in plasma field studies

Creators: None

Contributor: Karina Dale

16079
Systems toxicology of Atlantic cod

Type: Vitellogenin concentration in plasma field studies

Creators: None

Contributor: Karina Dale

16078
Systems toxicology of Atlantic cod

Type: Vitellogenin concentration in plasma field studies

Creators: None

Contributor: Karina Dale

16077
Systems toxicology of Atlantic cod

Type: Vitellogenin concentration in plasma field studies

Creators: None

Contributor: Karina Dale

16076
Systems toxicology of Atlantic cod

Type: Vitellogenin concentration in plasma field studies

Creators: None

Contributor: Karina Dale

16075
Systems toxicology of Atlantic cod

Type: Vitellogenin concentration in plasma field studies

Creators: None

Contributor: Karina Dale

16074
Systems toxicology of Atlantic cod

Type: Vitellogenin concentration in plasma field studies

Creators: None

Contributor: Karina Dale

16073
Systems toxicology of Atlantic cod

Type: Vitellogenin concentration in plasma field studies

Creators: None

Contributor: Karina Dale

16072
Systems toxicology of Atlantic cod

Type: Vitellogenin concentration in plasma field studies

Creators: None

Contributor: Karina Dale

16071
Systems toxicology of Atlantic cod

Type: Vitellogenin concentration in plasma field studies

Creators: None

Contributor: Karina Dale

16070
Systems toxicology of Atlantic cod

Type: Vitellogenin concentration in plasma field studies

Creators: None

Contributor: Karina Dale

2307 hidden items
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