In vivo II - GW and WY: Effects on cod lipid metabolism

Location: UiB Date: 8-20 June 2017 Compounds tested: WY-14,643 (PPAR alpha agonist), GW501516 (PPAR beta agonist) No. of test groups: 5 (Control 1: DMSO/saline/PEG, WY-14.643 High (40 mg/kg), WY-14.643 Low (4.0 mg/kg), GW501516 High (4.0 mg/kg), GW501516 Low (0.4 mg/kg)) No. of fish per group: 22

Aim: *To investigate effects on lipid metabolism in Atlantic cod ( Gadus morhua) mediated by peroxisome proliferator-activated receptors (Ppars) by in vivo exposure to two mammalian PPAR agonists.

DOI: 10.15490/fairdomhub.1.study.802.3

Zenodo URL: None

Created at: 13th Mar 2023 at 13:26

Contents

In vivo II - GW and WY Biometric data

No description specified

In vivo II -WY and GW biometrics

Biometric data from in vivo II experiment

  • In vivo II biometrics template_WYGW_SEEK.xlsx

17389

No description specified

17390

No description specified

17391

No description specified

17392

No description specified

17393

No description specified

17394

No description specified

17395

No description specified

17396

No description specified

17397

No description specified

17398

No description specified

17399

No description specified

17400

No description specified

17401

No description specified

17402

No description specified

17403

No description specified

17404

No description specified

17405

No description specified

17406

No description specified

17407

No description specified

17408

No description specified

17409

No description specified

17410

No description specified

17411

No description specified

17412

No description specified

17413

No description specified

17414

No description specified

17415

No description specified

17416

No description specified

17417

No description specified

17418

No description specified

17419

No description specified

17420

No description specified

17421

No description specified

17422

No description specified

17423

No description specified

17424

No description specified

17425

No description specified

17426

No description specified

17427

No description specified

17428

No description specified

17429

No description specified

17430

No description specified

17431

No description specified

17432

No description specified

17433

No description specified

17434

No description specified

17435

No description specified

17436

No description specified

17437

No description specified

17438

No description specified

17439

No description specified

17440

No description specified

17441

No description specified

17442

No description specified

17443

No description specified

17444

No description specified

17445

No description specified

17446

No description specified

17447

No description specified

17448

No description specified

17449

No description specified

17450

No description specified

17451

No description specified

17452

No description specified

17471

No description specified

17472

No description specified

17473

No description specified

17474

No description specified

17475

No description specified

17476

No description specified

17477

No description specified

17478

No description specified

17479

No description specified

17480

No description specified

17481

No description specified

17482

No description specified

17483

No description specified

17484

No description specified

17485

No description specified

17486

No description specified

17487

No description specified

17488

No description specified

17489

No description specified

17490

No description specified

17511

No description specified

17512

No description specified

17513

No description specified

17514

No description specified

17515

No description specified

17516

No description specified

17517

No description specified

17518

No description specified

17519

No description specified

17520

No description specified

17521

No description specified

17522

No description specified

17523

No description specified

17524

No description specified

17525

No description specified

17526

No description specified

17527

No description specified

17528

No description specified

17529

No description specified

17530

No description specified

17531

No description specified

RNA sequencing

Liver samples from Atlantic cod males.
Total RNA isolated using TRI Reagent (Fekadu Yadetie). Sequencing performed with Illumina Stranded mRNA (sequencing poly-A mRNA) at Genomic Core Facility at UiB/Haukeland (contact person Rita Holdhus).

The data sets are submitted to the ArrayExpress repository, and will be linked here.

RNA-Seq of Atlantic cod (Gadus morhua) liver treated with WY-14,643 and GW501516 in vivo

The aim of the exposure was to study the effects of activation of peroxisome proliferator-activated receptors (PPARs) in Atlantic cod (Gadus morhua), by injecting the fish with the compounds WY-14,643 and GW501516. Using luciferase reporter assay in vitro, we have shown that WY-14,643 activate Atlantic cod Ppara1 and Ppara2, while GW501516 activate Ppara1, Ppara2, and Pparb. The experimental set-up was as follows: Immature cod were injected at day 0 and day 4 with either high dose (40 mg/kg
...

  • E-MTAB-11490

Targeted lipidomics

Targeted lipidomic analysis was performed on plasma and isolated liver microsomes of eight male fish from solvent control (Control) and High-Dose groups (n = 8) at Cinta Porte’s lab at CSIC, Spain, using Flow Injection Analysis High-Resolution Mass Spectrometry (FIA-HRMS).

The data is submitted to the MetaboLights repository.

Targeted lipidomics data in MetaboLights repository

No description specified

  • descriptors

Proteomics data

The data is submitted to the PRIDE repository, and will be linked here.

Untargeted lipidomics

Untargeted lipidomics analysis was performed on plasma and liver samples of four male fish from each group (n = 4) at Per Bruheim’s lab at NTNU, Norway, using Liquid Chromatography Hybrid Quadrupole Mass Spectrometry (UPLC-HDMS).

Compound measurements from liver

No description specified

  • dCod Liver iv2-Compound Measurements.xlsx

Compound measurements from plasma

No description specified

  • dCod Plasma iv2_Compound measurements.xlsx

Untargeted liver lipid analysis_normalized values

Untargeted lipidomics analysis was performed on plasma and liver samples of four male fish from each group (n = 4) at Per Bruheim’s lab at NTNU, Norway, using Liquid Chromatography Hybrid Quadrupole Mass Spectrometry (UPLC-HDMS).

Normalisation: The fraction of each lipid compound among all measured lipids in every sample was calculated and was used in the downstream analysis.

  • Untargeted approach_Normalised liver lipids.xlsx

17391

No description specified

17393

No description specified

17398

No description specified

17399

No description specified

17411

No description specified

17412

No description specified

17413

No description specified

17414

No description specified

17434

No description specified

17437

No description specified

17438

No description specified

17442

No description specified

17473

No description specified

17474

No description specified

17475

No description specified

17477

No description specified

17511

No description specified

17514

No description specified

17516

No description specified

17521

No description specified

Fingerprints

These checksums allow you to check a Snapshot you have downloaded hasn't been modified. For details on how to use these please visit this guide

MD5: 3bb8c6dcc03499f7f9101d3708eb4e56

SHA1: b73ef97bb43ffd197a8c5b69d97bcd1a3c150cf4

Citation
Goksøyr, A., Eide, M., Karlsen, O. A., Yadetie, F., Zhang, X., Bartosova, Z., Fallahi, S., Blaser, N., Bruheim, P., Jonassen, I., Dale, K., & Söderström, S. (2023). In vivo II - GW and WY: Effects on cod lipid metabolism. FAIRDOMHub. https://doi.org/10.15490/FAIRDOMHUB.1.STUDY.802.3
Snapshots
Snapshot 3 (13th Mar 2023) DOI
Snapshot 2 (13th Mar 2023) DOI
Snapshot 1 (13th Mar 2023)
Activity

Views: 278   Downloads: 1

Created: 13th Mar 2023 at 13:26

Last updated: 13th Mar 2023 at 13:26

Powered by
(v.1.14.2)
Copyright © 2008 - 2023 The University of Manchester and HITS gGmbH